Immune transcriptome analysis in predatory beetles reveals two cecropin genes overexpressed in mandibles

García-Reina, Andrés; Rodríguez-García, María Juliana; Cuello, F.; Galián, José

doi:10.1016/j.jip.2020.107346

Cited by 1 publication

(1 citation statement)

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“…In Calomera littoralis beetles, cecropinA and cecropinB2 became highly expressed in the mandibles following E. coli lipopolysaccharide injection, as revealed using suppression subtractive hybridization (SSH) techniques (Garcia‐Reina et al . 2020). Based on the antibacterial efficacy of insect cecropins and cecropin‐like peptides, researchers have suggested the use of AMPs as potential alternatives to antibiotics for treatment of multidrug resistant infections (Brady et al .…”

Section: Introductionmentioning

confidence: 99%

Identification, in silico characterization, and expression analysis of Tenebrio molitor Cecropin‐2

Kojour

Jang

Edosa

et al. 2020

Entomological Research

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Antimicrobial peptides (AMPs) are considered to be candidate effectors for eliciting humoral immune responses against infectious pathogens in the host. Cecropins are α‐helical peptides of 30–40 amino acids, which are known to permeabilize bacterial membranes, and play authoritative roles in the innate immune system of insects. In the present study, we identified the full‐length open reading frame (ORF) encoding the Tenebrio molitor cecropin‐2 (TmCec2) gene using the Tribolium castaneum cecropin‐2 (TcCec2) gene to query a T. molitor Rnaseq database. Phylogenetic analysis revealed close identity of TmCec2 with TcCec2. TmCec2 was ubiquitously expressed in the insect during all developmental stages, with the highest expression observed in the adult. Tissue‐specific TmCec2 expression was highest in larval hemocytes and in the adult integument and hemocytes. Microbial challenge experiments revealed that TmCec2 was highly induced in response to gram‐positive and gram‐negative bacteria, and fungi. These data provide credible evidence for a putative role of TmCec2 in insect innate immunity against a plethora of pathogens.

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Section: Introductionmentioning

confidence: 99%