Immune-suppressive activity of punicalagin via inhibition of NFAT activation

Lee, Sang‐Ik; Kim, Byoungsoo; Kim, Kyoung-Shin; Lee, Sam-Keun; Shin, Kwang‐Soo; Lim, Jong-Soon

doi:10.1016/j.bbrc.2008.04.150

Cited by 77 publications

(50 citation statements)

References 16 publications

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“…Punicalagin, ellagic acid and gallic acid are the polyphenols found in pomegranate [4]. The main component of pomegranate husk is punicalagin [5]- [7]. Punicalagin is reported to have anti-inflammatory, anti-cancer and anti-atherosclerotic properties [7]- [9].…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity of Pomegranate Juice and Punicalagin

Omar¹,

Yousr²,

Grace³

et al. 2016

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Plant polyphenols are reported to have bioactive properties, which may be used for protection against diseases. Therefore, the aim of this research was to investigate the antioxidant activities of a pomegranate tannin polyphenol compound, punicalagin and pomegranate juice. The presence of punicalagin in pomegranate husk (US) and pomegranate juice (US & UK) was compared with a punicalagin standard using high performance liquid chromatography (HPLC) and liquid chromatography-mass spectroscopy (LC-MS) which are highly sensitive and selective analytical methods for the separation and identification of phenolic compounds and anthocyanins. Antioxidant mechanisms involving DPPH radical scavenging activity, hydrogen peroxide scavenging, ferrous chelating and reducing ability were also studied on pomegranate juice and standard punicalagin. The present study shows a high degree of similarity of HPLC and LC-MS results between the punicalagin commercial standard (Sigma Aldrich) and US pomegranate husk extracted with methanol. In contrast, in the methanol juice extract obtained from US and UK, higher hydrogen peroxide scavenging activity was achieved by 0.1 mg/ml from both punicalagin and pomegranate juice when compared with butylated hydroxytoluene (BHT) or trolox (p ≤ 0.01). Punicalagin and pomegranate juice exhibited ferrous chelating ability significantly lower than Ethylenediaminetetraacetic acid. These findings confirmed that punicalagin was present in pomegranate husk compared to pomegranate juice, as measured using a punicalagin standard. The antioxidant mechanism experiments concluded that, the pomegranate juice has a significantly higher radical scavenging activity in comparison with punicalagin (p ≤ 0.01). However, punicalagin showed significant ferrous chelating activity and reducing power ability in a dose-dependent manner as compared with pomegranate juice.

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Section: Introductionmentioning

confidence: 99%

Antioxidant Activity of Pomegranate Juice and Punicalagin

Omar¹,

Yousr²,

Grace³

et al. 2016

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show abstract

“…Furthermore, after 6h incubation, punicalagin (2.5-40 µM) significantly inhibited the activation of the nuclear factor activated T cells (NFAT), a transcription factor for IL-2 expression after T cell activation, in a dose-dependent way, in NFAT-Jurkat cells stimulated with PMA (10 ng/mL) and CI (1 µM) (S.I. Lee et al, 2008).…”

Section: In Vitro Effects On Immune Cellsmentioning

confidence: 98%

“…Additional in vitro studies have evaluated the potential role of pomegranate for preventing IBDs by using other types of cells involved in the intestinal immune response, such as murine splenic lymphocytes CD4+ T cells (S.I. Lee et al, 2008), RAW 264.7 murine macrophages (C.J. Lee et al, 2010;Panichayupakaranant et al, 2010), or the human basophilic cell line KU812 (Rasheed et al, 2009).…”

Section: In Vitro Anti-inflammatory Effects Of Pomegranate Phenolic Cmentioning

confidence: 99%

The Use of Pomegranate (Punica granatum L.) Phenolic Compounds as Potential Natural Prevention Against IBDs

Hollebeeck¹,

Larondelle²,

Schneider³

et al. 2012

Inflammatory Bowel Disease - Advances in Pathogenesis and Management

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“…For instance, Hoshino et al showed that PGE2 stimulates 14 the production of Aβ in cultured human neuroblastoma (SH-SY-5Y) cells [6]. Also, 15 reports have demonstrated elevated levels of PGE2 and COX-2 in the brains of AD 16 patients [7,8]. Inhibition of PGE2 production and COX-2 expression therefore 17 provides a critical target for reducing the contributions of neurons to the self- 18 perpetuating cycle of neuroinflammation.…”

mentioning

confidence: 99%

“…In this regard targeting BACE-1 and Aβ 32 production could be a potential strategy in slowing down the progression of AD. reduce nitric oxide and PGE2 production in intestinal cells [16,17]. In vitro and in vivo 39 studies showed that pomegranate produced significant reduction in egg albumin-40 induced hind paw inflammation following intraperitoneal and intracerebroventricular 41 administrations in rats, reduction in carrageenan-induced paw oedema, and NO 42 production and iNOS expression in RAW 264.7 cells [18,19].…”

mentioning

confidence: 99%