Immune Status 'in Children with Beta Thalassemia' in Correlation 'with Iron Overload': Single Center Egyptian Study

Hagag, Adel A; Elgamsy, Mohamed Abd Elaziz; El-Asy, Hassan M.; Gamal, Rasha; Elshahaby, Walid N.; El-Bar, Enaam S. Abd

doi:10.2174/1871530317666161107160213

Cited by 5 publications

(5 citation statements)

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“…These reports and our data suggested that the changes of IFN-γ-STAT2 based gene network induced by high level of iron might be the response to limit iron absorption or anti-infection. Our results also revealed that increasing iron reduced serum IgA, which was also observed in children with beta-thalassemia, indicating that iron overload might impair humoral immunity (Hagag et al, 2016). Collectively, high dietary iron changed intestinal gene expression profile, especially for immune genes.…”

Section: Discussionsupporting

confidence: 67%

Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model

Chen

Wang

et al. 2020

Front. Cell Dev. Biol.

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Iron is an essential metal for both animals and microbiota. In general, neonates and infants of humans and animals are at the risk of iron insufficiency. However, excess dietary iron usually causes negative impacts on the host and microbiota. This study aimed to investigate overloaded dietary iron supplementation on growth performance, the distribution pattern of iron in the gut lumen and the host, intestinal microbiota, and intestine transcript profile of piglets. Sixty healthy weaning piglets were randomly assigned to six groups: fed on diets supplemented with ferrous sulfate monohydrate at the dose of 50 ppm (Fe50 group), 100 ppm (Fe100 group), 200 ppm (Fe200 group), 500 ppm (Fe500 group), and 800 ppm (Fe800), separately, for 3 weeks. The results indicated that increasing iron had no significant effects on growth performance, but increased diarrheal risk and iron deposition in intestinal digesta, tissues of intestine and liver, and serum. High iron also reduced serum iron-binding capacity, apolipoprotein, and immunoglobin A. The RNA-sequencing analysis revealed that iron changed colonic transcript profile, such as interferon gamma-signal transducer and activator of transcription two-based anti-infection gene network. Increasing iron also shifted colonic and cecal microbiota, such as reducing alpha diversity and the relative abundance of Clostridiales and Lactobacillus reuteri and increasing the relative abundance of Lactobacillus and Lactobacillus amylovorus. Collectively, this study demonstrated that high dietary iron increased diarrheal incidence, changed intestinal immune response-associated gene expression, and shifted gut microbiota. The results would enhance our knowledge of iron effects on the gut and microbiome in piglets and further contribute to understanding these aspects in humans.

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Section: Discussionsupporting

confidence: 67%

Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model

Chen

Wang

et al. 2020

Front. Cell Dev. Biol.

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“…This could be due to hypoparathyroidism due to iron deposition in the parathyroid gland evidenced by elevated bone alkaline phosphatase in these patients [20]. Other factors also play a role including deficient calcium intake, IGF-I deficiency, delayed puberty and hypogonadism and decreased synthesis of 25-OH-D, due to hepatic siderosis [21] Furthermore, the osteoprotgrin serum level was statistically significantly higher in the cases group, this agreed with [18].…”

Section: Discussionmentioning

confidence: 60%

Estimation of Osteoprotgrin Level in B Thalassemia Children

Abd Elhameed Ibrahim,

Dabour,

Khashaba

et al. 2023

Benha Medical Journal

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“…The iron status deeply affects immunity, with iron-deficiency known to cause immune hyperactivity 35,36 prone initially towards Th2 [37][38][39] and being associated with atopic dermatitis 40 , allergic rhinitis 41,42 and asthma [43][44][45] . In contrast, in iron depleted conditions, immune cells seem to shift towards a more resilient state with macrophages polarizing towards an anti-inflammatory M2 sub-type in vivo and in vitro 46 , antigen presentation being decreased 11,46 , CD4+ counts 47 , and to a lesser extent CD8+ cells being negatively affected 48 . The lozenge was also spiked with vitamin A and zinc, essential contributors to immunity: Vitamin A supports mucosal regulation and predominantly anti-inflammatory pathways 10 , whereas zinc deficiencies potentially cause lymphopenia, as zinc functions as a second messenger for innate immunity.…”

Section: Discussionmentioning

confidence: 96%

The protective farm effect against allergies in a holoBLG based FSMP (food for specific medical purposes)-lozenge confers immune resilience in BALB/c mice

Afify,

Regner,

Pacios

et al. 2024

Preprint

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Background: We previously proposed the whey protein beta-lactoglobulin (BLG) loaded with iron-siderophore complexes as the active principle in the farm protective effect against allergies. A lozenge as food for specific medical purposes (FSMP) was formulated to assess its therapeutical efficacy in BALB/c mice and in-vitro experiments. Methods: Binding of iron-catechin into BLG was confirmed by spectroscopy and docking calculations. Serum IgE binding of children allergic to milk, or tolerating milk, was assessed to loaded (holo-) versus empty (apo-) BLG and for human mast cell degranulation. BLG and Bet v 1 double-sensitized mice were orally treated with the lozenge or placebo, and immunologically analysed after systemic allergen challenge. Human PBMCs of pollen allergic subjects were flow cytometrically assessed after stimulation with holoBLG in conjugation with catechin-iron complexes as ligands in a dietary supplement or with the apoBLG. Results: One major IgE- and T cell epitope were masked by catechin-iron complexes, which impaired IgE binding of milk allergic children and degranulation of mast cells. In mice, only supplementation with the lozenge reduced clinical reactivity to BLG and Bet v 1, promoted Tregs, and suppressed antigen presentation. In allergic subjects, stimulation of PBMCs with holoBLG led to a significant increase of intracellular iron in circulating CD14+ cells with significantly lower expression of HLADR and CD86 compared to their stimulation with apoBLG. Conclusion: The FSMP lozenge targeted antigen presenting cells and dampened immune activation in human immune cells and allergic mice in an antigen nonspecific manner, thereby conferring immune resilience against allergic symptoms.

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Immune Status 'in Children with Beta Thalassemia' in Correlation 'with Iron Overload': Single Center Egyptian Study

Abstract: Regular follow up of patients with beta thalassemia for detection of iron overload as it affects humeral and cell mediated immunity.

Cited by 5 publications

References 0 publications

Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model

Responses of Intestinal Microbiota and Immunity to Increasing Dietary Levels of Iron Using a Piglet Model

Estimation of Osteoprotgrin Level in B Thalassemia Children

The protective farm effect against allergies in a holoBLG based FSMP (food for specific medical purposes)-lozenge confers immune resilience in BALB/c mice

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