Immune responses with DNA vaccines encoded different gene fragments of severe acute respiratory syndrome coronavirus in BALB/c mice

Abstract

To analyze the immune responses of DNA vaccine encoded different gene fragments of severe acute respiratory syndrome coronavirus (SARS-Cov), SARS-Cov gene fragments of membrane (M), nucleocapsid (N), spike a (Sa), and spike b (Sb) proteins were cloned into pcDNA3.1 (Invitrogen) vector to form plasmids pcDNAM, pcDNAN, pcDNASa, and pcDNASb, respectively. After mice were immunized intramuscularly with pcDNAM, pcDNAN or pcDNASa-pcDNASb plasmid, blood was collected and serum was separated. Humoral immune response was detected with the enzyme-linked immunosorbent assay, and cellular immune response of SARS-Cov DNA vaccines was detected with lymphoproliferation assay and cytotoxic T lymphocyte assay. Results show that cellular and humoral immune responses can be detected after immunization with pcDNAM, pcDNAN or pcDNASa-pcDNASb plasmids in BALB/c mice. However, pcDNAM stimulated the highest cellular immune response than other plasmids, and pcDNASa-pcDNASb stimulated the highest humoral immune response in week 12. The present results not only suggest that DNA immunization with pcDNAM, pcDNAN or pcDNASa-pcDNASb could be used as potential DNA vaccination approaches to induce antibody in BALB/c mice, but also to illustrate that gene immunization with these SARS DNA vaccines different immune response characters.

Use of genetic immunization to generate a high-level antibody against rat dicarboxylate transporter

“…After four immunizations, we collected a high-level antibody against SDCT1. The serum antibody level was much higher than in many reports [13,25,26].…”

Enhanced Induction of SARS-CoV Nucleocapsid Protein-Specific Immune Response Using DNA Vaccination followed by Adenovirus Boosting in BALB/c Mice

“…To prevent and control the re-emerging and transmission of SARS-CoV, except for inactivated vaccine experiments [32][33][34] , several different research reports on DNA vaccines of SARSCoV have been recommended, such as S gene recombinant DNA vaccine [35][36][37][38] , N gene recombinant DNA vaccine [39,40] , and a combination of S, M and N gene recombinant DNA vaccines [41,42] . These genes are delivered and expressed by different plasmids or viral vectors and elicit different levels of humoral and cellular immune responses.…”

The Effect of Glycoprotein B DNA Immuniation on HSV-l Lethal Infection of BALB/c Mice

“…erpes simplex virus (HSV) is one of the most widespread pathogens through the world (1). It can cause many localized and systemic infections such as genital and oro labial lesions, blindness and fatal encephalitis (2-4).…”

“…Genetic immunization offers some advantages over other available vaccination methods, including presentation of native form of the protein, inducing CD8 + T lymphocytes proliferation and the ease in manipulation and production. Several successful reports have now described the use of DNA immunization in inducing effective immune responses against many pathogens (1,8,9). Regarding immunization against viruses, it seems that DNA vaccination is one of the most important approaches in developing effective vaccine production.…”

“…Regarding immunization against viruses, it seems that DNA vaccination is one of the most important approaches in developing effective vaccine production. Protective immunity can be induced by several HSV glycoproteins (1). Glycoproteins B and D (gB and gD) are the most important targets for immune system (10 (11).…”