Immune responses of orange-spotted grouper, Epinephelus coioides, against virus-like particles of betanodavirus produced in Escherichia coli

Lai, Yuxiong; Jin, Bao-Lei; Xu, Yunlin; Huang, Lijie; Huang, Ruxun; Zhang, Yong; Kwang, Jimmy; He, Jianguo; Xie, Jiehong

doi:10.1016/j.vetimm.2013.10.003

Cited by 64 publications

(38 citation statements)

References 52 publications

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“…Some studies of epitheliocystis have suggested that cyclic changes in temperature contribute to disease severity and prevalence, with greater epitheliocystis prevalence observed in warmer months which may more be a reflection of seasonal changes rather than water temperatures per se (Guevara Soto et al, 2016b). Groupers have been shown to produce innate and adaptive immune activation in response to infection with other intracellular pathogens (Huang et al, 2011;Wei et al, 2012;Lai et al, 2014). Three E. coioides broodstock (EC-May-14) sampled at a different facility were negative for epitheliocystis, suggesting that the bacterium could be introduced to the ponds or JCU facility via seawater exchange (see methods) or that there is agedependent effect on disease prevalence and severity.…”

Section: Resultsmentioning

confidence: 99%

Culture‐independent genomics of a novel chlamydial pathogen of fish provides new insight into host‐specific adaptations utilized by these intracellular bacteria

Taylor-Brown

Pillonel

Bridle

et al. 2017

Environmental Microbiology

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Several Chlamydiales families are associated with epitheliocystis, a common condition of the fish gill epithelium. These families share common ancestors with the Chlamydiaceae and environmental Chlamydiae. Due to the lack of culture systems, little is known about the biology of these chlamydial fish pathogens. We investigated epitheliocystis in cultured Orange-spotted grouper (Epinephelus coioides) from North Queensland, Australia. Basophilic inclusions were present in the gills of 22/31 fish and the presence of the chlamydial pathogen in the cysts was confirmed by in situ hybridization. Giant grouper (Epinephelus lanceolatus) cultured in the same systems were epitheliocystis free. 16S rRNA gene sequencing revealed a novel member of the Candidatus Parilichlamydiaceae: Ca. Similichlamydia epinephelii. Using metagenomic approaches, we obtained an estimated 68% of the chlamydial genome, revealing that this novel chlamydial pathogen shares a number of key pathogenic hallmarks with the Chlamydiaceae, including an intact Type III Secretion system and several chlamydial virulence factors. This provides additional evidence that these pathogenic mechanisms were acquired early in the evolution of this unique bacterial phylum. The identification and genomic characterization of Ca. S. epinephelii provides new opportunities to study the biology of distantly-related chlamydial pathogens while shining a new light on the evolution of pathogenicity of the Chlamydiaceae.

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Section: Resultsmentioning

confidence: 99%

Culture‐independent genomics of a novel chlamydial pathogen of fish provides new insight into host‐specific adaptations utilized by these intracellular bacteria

Taylor-Brown

Pillonel

Bridle

et al. 2017

Environmental Microbiology

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“…The expression of the viral protein as virus-like particles (VLPs) has also been achieved in the yeast Yarrowia lipolytica [337]. The efficacy of recombinant proteins or VLPs has also been assessed in other studies [338][339][340][341][342][343][344] and the stability of the VLP vaccine after lyophilization has been demonstrated [345].…”

Section: Preventionmentioning

confidence: 99%

Betanodavirus and VER Disease: A 30-year Research Review

2020

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The outbreaks of viral encephalopathy and retinopathy (VER), caused by nervous necrosis virus (NNV), represent one of the main infectious threats for marine aquaculture worldwide. Since the first description of the disease at the end of the 1980s, a considerable amount of research has gone into understanding the mechanisms involved in fish infection, developing reliable diagnostic methods, and control measures, and several comprehensive reviews have been published to date. This review focuses on host-virus interaction and epidemiological aspects, comprising viral distribution and transmission as well as the continuously increasing host range (177 susceptible marine species and epizootic outbreaks reported in 62 of them), with special emphasis on genotypes and the effect of global warming on NNV infection, but also including the latest findings in the NNV life cycle and virulence as well as diagnostic methods and VER disease control.

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“…Given the similarity to their native viral capsids, VLPs provide an excellent platform for displaying viral epitopes [ 146 , 152 ]. This property was demonstrated by Lai et al [ 153 ] who produced VLPs for NNV expressed in E. coli and showed that NNV failed to infect the Asian sea bass cells that were exposed to the VLPs prior to infection, suggesting that the cell surface receptors were occupied by the VLP-epitopes blocking the wild type virus from entering the cells and thereby protected the cells from developing cytopathic effect (CPE) while control cells not exposed to VLPs developed full CPE. Liu et al [ 146 ] showed that VLPs generated from NNV induced high antibody responses that lasted for more than five months, similar to those produced by the native wild type virus, which were correlated with long-term protection in vaccinated orange spotted grouper.…”

Section: Extracellular Antigen Delivery Systemsmentioning

confidence: 88%

“…Liu et al [ 146 ] showed that VLPs generated from NNV induced high antibody responses that lasted for more than five months, similar to those produced by the native wild type virus, which were correlated with long-term protection in vaccinated orange spotted grouper. Similarly, Lai et al [ 153 ] produced VLPs in E. coli for NNV that expressed high antibody levels, which were correlated with IgM, MHC-II, and CD4 levels in vaccinated fish. These observations suggest VLPs induce the expression of CD4 responses through the MHC-II pathways in a similar pattern to those induced by subunit vaccines in Mammalia [ 154 ].…”

Section: Extracellular Antigen Delivery Systemsmentioning

confidence: 99%

A Review of Intra- and Extracellular Antigen Delivery Systems for Virus Vaccines of Finfish

Munang’andu

Evensen

2015

Journal of Immunology Research

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Vaccine efficacy in aquaculture has for a long time depended on evaluating relative percent survival and antibody responses after vaccination. However, current advances in vaccine immunology show that the route in which antigens are delivered into cells is deterministic of the type of adaptive immune response evoked by vaccination. Antigens delivered by the intracellular route induce MHC-I restricted CD8+ responses while antigens presented through the extracellular route activate MHC-II restricted CD4+ responses implying that the route of antigen delivery is a conduit to induction of B- or T-cell immune responses. In finfish, different antigen delivery systems have been explored that include live, DNA, inactivated whole virus, fusion protein, virus-like particles, and subunit vaccines although mechanisms linking these delivery systems to protective immunity have not been studied in detail. Hence, in this review we provide a synopsis of different strategies used to administer viral antigens via the intra- or extracellular compartments. Further, we highlight the differences in immune responses induced by antigens processed by the endogenous route compared to exogenously processed antigens. Overall, we anticipate that the synopsis put together in this review will shed insights into limitations and successes of the current vaccination strategies used in finfish vaccinology.

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Immune responses of orange-spotted grouper, Epinephelus coioides, against virus-like particles of betanodavirus produced in Escherichia coli

Cited by 64 publications

References 52 publications

Culture‐independent genomics of a novel chlamydial pathogen of fish provides new insight into host‐specific adaptations utilized by these intracellular bacteria

Culture‐independent genomics of a novel chlamydial pathogen of fish provides new insight into host‐specific adaptations utilized by these intracellular bacteria

Betanodavirus and VER Disease: A 30-year Research Review

A Review of Intra- and Extracellular Antigen Delivery Systems for Virus Vaccines of Finfish

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