Immune responses of mice against recombinant bovine herpesvirus 5 glycoprotein D

Dummer, Luana Alves; Araujo, Itauá Leston; Finger, Paula Fonseca; Santos, Alceu Gonçalves dos; Rosa, Matheus Costa da; Conceição, Fabrício Rochedo; Fischer, Geferson; Hurk, Sylvia van Drunen Littel‐van den

doi:10.1016/j.vaccine.2014.03.011

Cited by 31 publications

(25 citation statements)

References 48 publications

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“…The evaluation of total IgG antibodies against BoHV-5 was performed by ELISA (Dummer et al, 2014). Briefly, plates (Nunc) were sensitized with 50µl of a suspension containing recombinant BoHV-5 glycoprotein D at a concentration of 2mg/ml diluted in carbonatebicarbonate buffer (pH 9.6) at 4ºC for 18h.…”

Section: Methodsmentioning

confidence: 99%

Saccharomyces boulardii modulates and improves the immune response to Bovine Herpesvirus type 5 Vaccine

Roos

Ávila

Sturbelle

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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There have been significant efforts towards the development of more efficient vaccines for animal health. A strategy that may be used to improve vaccine efficacy is the use of probiotics to enhance the immune response of the host, leading to increased immunogenicity of antigen preparations. Bovine herpesvirus 5 (BoHV-5) is an example of an important animal pathogen for which vaccines have provided only limited protection. In this study, we examined the use of the probiotic Saccharomyces boulardii (Sb) as a potential adjuvant to improve vaccine efficiency. We found that the supplemented animals exhibited an enhanced systemic IgG antibody response toward a Th1 response in favor of IgG2a and increased mRNA expression levels of the cytokines IFN-y, IL-12, IL-17 and IL-10 in the spleen. These results suggest that Sb supplementation may provide a promising means for improving the efficiency of vaccines, particularly those that rely on a cell-mediated immune response.

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Section: Methodsmentioning

confidence: 99%

Saccharomyces boulardii modulates and improves the immune response to Bovine Herpesvirus type 5 Vaccine

Roos

Ávila

Sturbelle

et al. 2018

Arq. Bras. Med. Vet. Zootec.

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“…Beta-actin and GAPDH genes were used as endogenous reference controls, but subsequently, b-actin was selected as the internal reference standard based on its efficiency (M-value of 1.8 and 1.98 for GAPDH and b-actin, respectively). The primer sequence used was described elsewhere [10].…”

Section: Ex Vivo Spleen Stimulation and Cytokine Transcriptsmentioning

confidence: 99%

Identification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2

Pinheiro

Roloff

Moreira

et al. 2018

Vaccine

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The parasite Neospora caninum is the main cause of abortion in cattle in many countries around the world, so a vaccine is a rational approach method for the control of the disease. An effective vaccine should be able to prevent both, the horizontal and vertical transmission of N. caninum. In this study, the immune vaccinal response of the recombinant protein rNcSRS2 of N. caninum expressed in Pichia pastoris and formulated with water-in-oil emulsion, xanthan gum, and alum hydroxide was assessed in an experimental murine model. Groups of 10 Balb/c mice were subcutaneously inoculated with two doses of prNcSRS2 twenty-one days apart. After the second immunization, four mice from each group were euthanized, and splenocytes were stimulated ex vivo with recombinant protein. The IgG dynamics were evaluated by indirect ELISA, and the splenocytes cytokines transcription by qPCR. All groups elicited specific antibodies against prNcSRS2, with the water-in-oil group showing significantly (p .05) elevated titers compared to the other groups. The prNcSRS2 protein alone did not induce a significant ex vivo splenic transcription level of IFN-c, TNF-a, IL-4, IL-10, and IL-12 cytokines, except for IL-17A, and the adjuvant associations with the prNcSRS2 protein induced different cytokine transcription profiles. The water-inoil emulsion modulated the expression of TNF-a; the xanthan gum modulated IL-4, IL-10, and IL-12; and alum hydroxide modulated IFN-c, TNF-a, IL-4, IL-10, and IL-12. In conclusion, it was found that the association of the recombinant prNcSRS2 protein with different adjuvants induced different levels of specific antibody, and a distinct splenic cytokine profile in an adjuvant-dependent manner. The mechanisms of adjuvancity activity is complex, so adjuvant formulation may help in the design of efficient vaccine to control Neosporosis.

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“…Furthermore, we expressed the secreted form of gD from BoHV-5 in P. pastoris by removing the transmembrane anchor of the native gD [ 65 ]. Recombinant BoHV-5 gD when formulated with oil-based adjuvants induced neutralizing antibodies in mice [ 25 ] and bovines (Itauá Leston Araújo, 2014 - unpublished observations) after vaccination.…”

Section: Vaccines Designed With Gdmentioning

confidence: 99%

“…In consideration of the molecular homology between these viruses [ 7 ] most of this review will describe studies with BoHV-1 and BoHV-5. However, although multiple outbreaks of infection by BoHV-5 have been described [ 22 - 24 ] and BoHV-5 infection leads to more serious disease, there are fewer reports on vaccine development strategies against BoHV-5 than against BoHV-1 [ 18 , 25 ]. Where appropriate, studies with gD from other alphaherpesviruses will be described to illustrate the recent progress with gD-based vaccines in general.…”

Section: Introductionmentioning

confidence: 99%

Bovine herpesvirus glycoprotein D: a review of its structural characteristics and applications in vaccinology

Dummer

Hurk

2014

Vet Res

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The viral envelope glycoprotein D from bovine herpesviruses 1 and 5 (BoHV-1 and -5), two important pathogens of cattle, is a major component of the virion and plays a critical role in the pathogenesis of herpesviruses. Glycoprotein D is essential for virus penetration into permissive cells and thus is a major target for virus neutralizing antibodies during infection. In view of its role in the induction of protective immunity, gD has been tested in new vaccine development strategies against both viruses. Subunit, DNA and vectored vaccine candidates have been developed using this glycoprotein as the primary antigen, demonstrating that gD has the capacity to induce robust virus neutralizing antibodies and strong cell-mediated immune responses, as well as protection from clinical symptoms, in target species. This review highlights the structural and functional characteristics of BoHV-1, BoHV-5 and where appropriate, Human herpesvirus gD, as well as its role in viral entry and interactions with host cell receptors. Furthermore, the interactions of gD with the host immune system are discussed. Finally, the application of this glycoprotein in new vaccine design is reviewed, taking its structural and functional characteristics into consideration.

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Immune responses of mice against recombinant bovine herpesvirus 5 glycoprotein D

Cited by 31 publications

References 48 publications

Saccharomyces boulardii modulates and improves the immune response to Bovine Herpesvirus type 5 Vaccine

Saccharomyces boulardii modulates and improves the immune response to Bovine Herpesvirus type 5 Vaccine

Identification of suitable adjuvant for vaccine formulation with the Neospora caninum antigen NcSRS2

Bovine herpesvirus glycoprotein D: a review of its structural characteristics and applications in vaccinology

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