Immune responses in vitro. I. Culture conditions for antibody synthesis

Click, Robert E.; Benck, Loretta; Alter, Barbara J.

doi:10.1016/0008-8749(72)90165-7

Cited by 383 publications

(114 citation statements)

References 24 publications

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“…of Becton, Dickinson & Co., Oxnard, Calif.) containing 25 × 10 s purified Fc-receptor-negative (FcR-) Tresponder cells (carbonyl-iron-and magnet-treated, followed by passage through Ig-anti-Ig columns) and 10 × 106 anti-Thy-1.2-and-complement-treated parental or F1 hybrid stimulator cells (21,26). The medium consisted of 5% fetal calf serum (FCS) in a 1:1 mixture of Eagle's high amino acid medium (28) and R medium (ER); the culture time was 4-5 d. Cells were harvested and further purified using FIP (26). Such activated T cells were 30-95% blasts, >98% Thy-l.2, < 1% Ig +, <0.5% Fc-receptor-positive, and without antibody-dependent cell-mediated cytotoxic (ADCC) potential (22,23,26,27).…”

Section: Preparation and Test Of Lymphocytementioning

confidence: 99%

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Rubin¹,

Hertel-Wulff²,

Kimura³

1979

The Journal of Experimental Medicine

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The present study describes the qualitative reactions of a xenogeneic anti-idiotype (Id) antiserum produced in a mouse-gamma-globulin-tolerant rabbit (5,936) against B6 anti-CBA IgG antibodies. The results showed that such an anti-Id antiserum reacts specifically against anti-H-2k antibodies and against H-2k alloantigen-activated T cells from the following pairs of congenic mice: B10 (H-2b) and B10.D2 (H-2d); and A.BY (H-2b) and A.SW (H-2s), but not against C3H.SW (H-2b) and C3H.OH (H-2o); and BALB/b (H-2b) and BALB/c (H-2d). CB 20 (BALB/c mice with the Ig-1b allotype) anti-CBA T blasts also express idiotypic determinants that react with rabbit 5,936 antiserum. Thus, positive reactions are obtained between rabbit 5,936 anti-Id antiserum and anti-H-2k IgG preparations and T blasts from mice carrying the Ig-1b or Ig-1e allotype, but not from mice carrying the Ig-1a allotype. These reactions are qualitatively independent of the H-2 genotype of the Id-producing mice. Such a finding strongly suggests that the Id-bearing receptor molecules on mouse T cells are coded for by genes that are associated with the Ig heavy-chain-linkage group and not to the mouse histocompatibility complex. Furthermore, the anti-Id antibodies studied react preferentially against anti-H-2k antibodies or T cells with specificity toward the IAk-region-associated serological specificities. Thus, genes associated with the Ig heavy-chain-linkage group seem to be structural genes for at least T-cell receptors with specificity for IA-region-coded membrane antigens.

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Section: Preparation and Test Of Lymphocytementioning

confidence: 99%

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Rubin¹,

Hertel-Wulff²,

Kimura³

1979

The Journal of Experimental Medicine

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“…Spleen and lymph node cells or purified lymphocytes from blood (Ficoll-Isopaque purified [1]) were used as responder cells and spleen cells as stimulator cells. Eagle's high amino acid medium (14) with the double amount of l-glutamine and complemented with penicillin, streptomycin, 0.5% (final dilution) of normal fresh BN rat serum, and 2.5 × 10 -5 M (final concentration) of 2-mercaptoethanol was used. The cultures were pulsed as indicated in the Tables with 1 ~Ci of [3H]TdR.…”

Section: Mixed Leukocyte Cultures (Mlc)mentioning

confidence: 99%

Specific transplantation tolerance induced by autoimmunization against the individual's own, naturally occurring idiotypic, antigen-binding receptors.

Binz¹,

Wigzell²

1976

The Journal of Experimental Medicine

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Serum or urine from normal adult Lewis rats can be shown to contain detectable amounts of idiotypic, antigen-binding receptors with specificity for the major histocompatibility complex locus antigens of the rat, the Ag-B locus antigens. Such purified naturally occurring receptor molecules, be they of T- or B-lymphocyte origin, can be used in a polymerized form to provoke the production of auto-anti-idiotypic antibodies when injected back into normal Lewis rats. As a consequence of this autoimmunity, lymphocytes of these Lewis rats can be shown to be depleted of cells carrying the relevant idiotypic receptors signifying reactivity against a given Ag-B locus-determined antigen(s). This specific lack of idiotypic lymphocytes is manifested as a selective loss of reactivity against the relevant Ag-B-incompatible antigens as measured by graft versus host or MLC reactions. Furthermore, autoimmune Lewis rats display specific transplantation tolerance against the skin grafts from the relevant strain, as demonstrated by specific prolongation of graft survival. A further indication of the specific tolerence state of these rats comes from the highly reduced ability to produce circulating antibodies against the relevant Ag-B antigens. No side effects of these autoimmunization procedures have been noted so far. It would thus seem clear that a prolonged state of specific transplantation tolerance can be achieved via autoimmunization against the individual's naturally occurring idiotypic, antigen-binding receptors.

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“…Since Click et al have reported that 2 mercaptoethanol (2-ME) augments an in vitro antibody response in murine lympho cytes (1), this simple thiol compound has been widely used as an additive to the culture medium for eliciting an antibody response efficiently. Broome and Jeng have demon strated that a variety of thiol and disulfide compounds could stimulate the in vitro growth of L1210, a murine lymphoma (2).…”

mentioning

confidence: 99%

Augmentation of in Vitro Antibody Response by Disulfide Compounds I. Comparison between Intermolecular and Intramolecular Disulfides

Ohmori

Yamauchi

Yamamoto

1985

Japanese Journal of Pharmacology

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Abstract-The following disulfide compounds: 2-hydroxyethyldisulfide (2-ME,,X), 2-aminoethyldisulfide (cystamine) and oxidized dithiothreitol (DTToX) were found to augment the in vitro antibody response to sheep erythrocytes in murine lymphocytes as effectively as their reduced forms when they were added to the culture medium.We, however, found out that the mode of action of DTToX was greatly different from that of 2-ME,,,, or cystamine. 2-ME,,,, and cystamine showed augmenting effects on the proliferative response to lipopolysaccharide and on the uptake of (35S) -cystine by the lymphocytes.In contrast to these intermolecular disulfides, DTToX, an intramolecular disulfide compound, was found to be inactive in these systems.2-ME,,,, and cystamine, but not DTToX, were reduced to thiol forms by the intact lymphocytes or by the cell homogenate.Thus, it is likely that DTToX did not behave as the reduced form in the lymphocyte culture in contrast to 2-ME,, and cystamine, suggesting that the disulfide form itself plays an important role in augmenting effects of DTToX.

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Immune responses in vitro. I. Culture conditions for antibody synthesis

Cited by 383 publications

References 24 publications

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Alloantigen-specific idiotype-bearing receptors on mouse T lymphocytes. I. Specificity characterization and genetic association with the heavy-chain IgG allotype.

Specific transplantation tolerance induced by autoimmunization against the individual's own, naturally occurring idiotypic, antigen-binding receptors.

Augmentation of in Vitro Antibody Response by Disulfide Compounds I. Comparison between Intermolecular and Intramolecular Disulfides

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