Immune responses in mice against herpes simplex virus: mechanisms of protection against facial and ganglionic infections

Zweerink, Hans J.; Martínez, Daynelis Pardo; Lynch, RM; Stanton, L W

doi:10.1128/iai.31.1.267-275.1981

Cited by 14 publications

(3 citation statements)

References 29 publications

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“…The immune response to the GP130 glycoprotein complex was quantitated with material from HSV-2 (Curtiss)-infected primary rabbit kidney cells that specifically bound to the lectin from L. culinaris. It has been found that more than 85% of the bound radiolabeled virus-specific polypeptides consist of the GP130 complex (25).…”

Section: Figmentioning

confidence: 99%

Virus-specific antibodies in sera from patients with genital herpes simplex virus infection

Zweerink¹,

Corey²

1982

Infect Immun

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Virus-specific antibodies against a number of herpes simplex virus type 2 antigens were determined by radioimmunoprecipitation assays in sequential serum samples obtained from 12 patients with initial genital herpes simplex virus infection. The progressive appearance of antibodies to virus-specific antigens was observed; antibodies against a 130,000-molecular-weight glycoprotein complex appeared first, followed by antibodies against the major nucleocapsid polypeptide and then antibodies against a number of other viral antigens, including a polypeptide with a molecular weight of 62,000. Patients who developed a wide variety of antibodies to viral polypeptides shortly after resolution of their initial episode seemed to experience more severe initial infections and more recurrences than did those who reacted poorly with these virus-specific antigens. This was most apparent with respect to antibodies to virus-specific polypeptides with molecular weights between 30,000 and 43,000. Antibody specificity did not change during the course of follow-up regardless of whether serum samples were taken shortly before, during, or after recurrent episodes. Glycoprotein-specific antibodies were quantitated with the purified 130,000-molecular-weight glycoprotein material. No significant fluctuations in these antibody titers were observed before or after recurrences of the disease.

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Section: Figmentioning

confidence: 99%

Virus-specific antibodies in sera from patients with genital herpes simplex virus infection

Zweerink¹,

Corey²

1982

Infect Immun

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“…Immunoprecipitating antibody specific for the three major HSV glycoproteins are readily detected in sera from patients with recurrent herpetic lesions and in sera derived from animals infected with live or inactivated virus preparations (8,9,11,35). However, the relative importance of antibodies directed against individual glycoproteins in protective immunity is uncertain.…”

mentioning

confidence: 99%

Immunogenicity of herpes simplex virus glycoproteins gC and gB and their role in protective immunity

Glorioso¹,

Schröder²,

Kümel³

et al. 1984

J Virol

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The relative antigenicity of the individual herpes simplex virus type 1 (KOS) glycoproteins gC and gB was analyzed in BALB/c mice by using KOS mutants altered in their ability to present these antigens on cell surface membranes during infection. The mutants employed were as follows: syn LD70, a non-temperaturesensitive mutant defective in the synthesis of cell surface membrane gC; tsF13, a temperature-sensitive mutant defective in the processing of the precursor form of gB to the mature cell surface form at 39°C; and ts606, an immediate early temperature-sensitive mutant defective in the production of all early and late proteins including the glycoproteins. By comparing the relative susceptibility to immunolysis of mouse 3T3 cells infected at 39°C with wild-type virus, presenting the full complement of the glycoprotein antigens, gC, gB, and gD, with target cells infected with mutants presenting only subsets of these antigens, we determined that a major portion of cytolytic antibody contained in hyperimmune anti-herpes simplex virus type 1 (KOS) mouse antiserum was directed against glycoproteins gC and gB. The relative immunogenicity of wild-type and mutant virus-infected cells also was compared in BALB/c mice. Immunogen lacking the mature form of gB induced a cytolytic antibody titer comparable to that of the wild-type virus, whereas that lacking the mature form of gC showed a 70% reduction in titer. The absence of the mature cell surface forms of gB and gC in immunogen preparations resulted in a 4-to 15-fold reduction in virus neutralizing titer. Animals immunized with ts606-infected cells (39°C) induced relatively little virus-specific cytolytic and neutralizing antibody. Analysis of the glycoprotein specificities of these antisera by radioimmunoprecipitation showed that the antigens immunoprecipitated reflected the viral plasma membrane glycoprotein profiles of the immunogens. The absence of the mature forms of gC or gB in the immunizing preparation did not appreciably affect the immunoprecipitating antibody response to other antigens. Mice immunized with wildtype and mutant virus-infected cells were tested for their resistance to intracranial and intraperitoneal challenge with the highly virulent WAL strain of herpes simplex virus type 1. Despite the observed alterations in serum virus-specific antibody induced with the individual immunogens, all animals survived an intraperitoneal challenge of 10 50% lethal doses. However, differences in the survival of animals were obtained upon intracranial challenge. Protection could be induced most efficiently with wild-type virus, less well with the gC and gB mutants, and the least with the early mutant ts606. The level of protection did not strictly correlate with the relative titers of virus-neutralizing antibodies; however, in general, a reduction in titer was associated with increased susceptibility to virus challenge. These findings suggest that antibody with neutralizing activity in vitro may contribute to protection against neurological herpes simplex virus infec...

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“…The reason for increased susceptibility of nude mice to HSV is complex . Sev eralstudies have confirmed that nude mice fail to produce neutralizing or immune percipitation antibody in response to HSV infection (1,3,21,25,28). We have now documented for the first time that they also fail to produce anti-HSV ADCC antibody (Table 4).…”

Section: Discussionmentioning

confidence: 60%

Antibody‐Dependent Cellular Cytotoxicity and Natural Killer Cytotoxicity of Peritoneal Cells from Nude Mice to Herpes Simplex Virus‐Infected Cells

Kohl

Tang

Loo

1984

Microbiology and Immunology

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Nude BALB/c mice (athymic) were more susceptible to fatal herpes simplex virus (HSV) than normal BALB/c mice (P=0.002). The peritoneal cells of nude mice mediated levels of antibody‐dependent cellular cytotoxicity (ADCC) of equal or greater magnitude than cells from normal BALB/c, heterozygote nu/+, or C57BL/6 mice. Unstimulated natural killer cytotoxicity of peritoneal cells from nude mice was higher (P<0.05) than that mediated by cells from C57BL/6 mice. Nude mice failed to make anti‐HSV ADCC antibody 6 to 14 days post HSV inoculation, at times when nu/+, BALB/c, and C57BL/6 mice produced antibody. Passive reconstitution of nude mice with high titer intraperitoneal anti‐HSV immune globulin provided circulating anti‐HSV ADCC antibody and significant protection against lethal HSV infection.

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Immune responses in mice against herpes simplex virus: mechanisms of protection against facial and ganglionic infections

Cited by 14 publications

References 29 publications

Virus-specific antibodies in sera from patients with genital herpes simplex virus infection

Virus-specific antibodies in sera from patients with genital herpes simplex virus infection

Immunogenicity of herpes simplex virus glycoproteins gC and gB and their role in protective immunity

Antibody‐Dependent Cellular Cytotoxicity and Natural Killer Cytotoxicity of Peritoneal Cells from Nude Mice to Herpes Simplex Virus‐Infected Cells

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