Immune responses elicited in rainbow trout through the administration of infectious pancreatic necrosis virus-like particles

Martinez-Alonso, S.; Vakharia, Vikram N.; Saint-Jean, Sylvia Rodríguez; Pérez-Prieto, Sara I.; Tafalla, Carolina

doi:10.1016/j.dci.2011.07.010

Cited by 22 publications

(13 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is in agreement with past reports showing upregulation of CD4-1 transcripts in lymphoid organs of salmon and trout in response to immunogenic stimuli (16,(48)(49)(50)(51)(52)(53)(54), usually interpreted as an indicator of CD4 + T cell proliferation. Proliferation also has been reported for CD4-1 + cells isolated from ginbuna carp during allogeneic MLR as well as after Ag-specific stimulation (18).…”

Section: Discussionsupporting

confidence: 82%

Isolation and Characterization of Salmonid CD4+ T Cells

Maisey

Montero

Corripio-Miyar

et al. 2016

The Journal of Immunology

View full text Add to dashboard Cite

This study reports the isolation and functional characterization of rainbow trout (Oncorhynchus mykiss) CD4-1+ T cells and the establishment of an IL-15–dependent CD4-1+ T cell line. By using Abs specific for CD4-1 and CD3ε it was possible to isolate the double-positive T cells in spleen and head kidney. The morphology and the presence of transcripts for T cell markers in the sorted CD4-1+CD3ε+ cells were studied next. Cells were found to express TCRα, TCRβ, CD152 (CTLA-4), CD154 (CD40L), T-bet, GATA-3, and STAT-1. The sorted CD4-1+ T cells also had a distinctive functional attribute of mammalian T lymphocytes, namely they could undergo Ag-specific proliferation, using OVA as a model Ag. The OVA-stimulated cells showed increased expression of several cytokines, including IFN-γ1, IL-4/13A, IL-15, IL-17D, IL-10, and TGF-β1, perhaps indicating that T cell proliferation led to differentiation into distinct effector phenotypes. Using IL-15 as a growth factor, we have selected a lymphoid cell line derived from rainbow trout head kidney cells. The morphology, cell surface expression of CD4-1, and the presence of transcripts of T cell cytokines and transcription factors indicated that this is a CD4-1+ T cell line. To our knowledge, this is the first demonstration of the presence of CD4-1+CD3ε+ T cells in salmonids. As in mammals, CD4-1+ T cells may be the master regulators of immune responses in fish, and therefore these findings and the new model T cell line developed will contribute to a greater understanding of T cell function and immune responses in teleost fish.

show abstract

Section: Discussionsupporting

confidence: 82%

Isolation and Characterization of Salmonid CD4+ T Cells

Maisey

Montero

Corripio-Miyar

et al. 2016

The Journal of Immunology

View full text Add to dashboard Cite

show abstract

“…Rainbow trout infected with IPNV by bath challenge showed a decrease in serum IgM levels, while no changes were detected in IgT titers (60). Interestingly, intracoelomic injections of rainbow trout with IPNV viral-like particles induced a strong mobilization of IgM + and IgT + cells to the celomic cavity (61). Intracoelomic vaccination of rainbow trout against the bacteria Yersinia ruckeri induced IgM upregulation, but failed to affect IgT expression (62).…”

Section: Discussionmentioning

confidence: 99%

Differential Modulation of IgT and IgM upon Parasitic, Bacterial, Viral, and Dietary Challenges in a Perciform Fish

et al. 2016

View full text Add to dashboard Cite

Three different immunoglobulin (Ig) isotypes can be found in teleost fish, IgM, IgD, and the teleost-specific IgT. IgM is considered to have a systemic activity, and IgT is attributed a mucosal role, similar to mammalian IgA. In this study, the complete sequence of gilthead sea bream IgM and IgT in their membrane (m) and soluble (s) forms are described for the first time in a perciform fish. Their constitutive gene expression is analyzed in different tissues, and their regulation upon viral, bacterial, parasitic, mucosal vaccination and dietary challenges are studied. GCB IgM and IgT have the prototypical structure when compared to other fish Igs. The constitutive expression of sIgM was the highest overall in all tissues, whereas mIgT expression was highest in mucosal tissues, such as gills and intestine. IgM and IgT were differentially regulated upon infection. IgT was highly upregulated locally upon infection with the intestinal parasite Enteromyxum leei or systemically after Nodavirus infection. Long-term intestinal parasitic infections increased the serum titer of both isotypes. Mucosal vaccination against Photobacterium damselae subsp. piscicida finely regulated the Ig response inducing a systemic increase of IgM titers in serum and a local IgT response in skin mucus when animals were exposed to the pathogen by bath challenge. Interestingly, plant-based diets inhibit IgT upregulation upon intestinal parasitic challenge, which was related to a worse disease outcome. All these results corroborate the mucosal role of IgT and emphasize the importance of a finely tuned regulation of Ig isotypes upon infection, which could be of special interest in vaccination studies.

show abstract

“…For each mRNA, gene expression was normalized by the elongation factor 1a (EF-1a) expression in each sample and expressed as 2 2DCt , where DCt is determined by subtracting the EF-1a Ct value from the target Ct as previously described [23]. All the primers used had already been optimized in previous studies [23][24][25]. Amplifications were performed in duplicate and negative controls with no template were always included in the reactions.…”

Section: Evaluation Of Immune Gene Expression By Real Time Pcrmentioning

confidence: 99%

“…Reaction mixtures containing 5 ml of 26 SYBR Green supermix, 1 ml of each primer (1 mM each) and 2 ml of cDNA template were incubated for 10 min at 95uC, followed by 40 amplification cycles (30 s at 95uC and 1 min at 60uC) and a dissociation cycle (30 s at 95uC, 1 min 60uC and 30 s at 95uC). For each mRNA, gene expression was normalized by the elongation factor 1a (EF-1a) expression in each sample and expressed as 2 2DCt , where DCt is determined by subtracting the EF-1a Ct value from the target Ct as previously described [23]. All the primers used had already been optimized in previous studies [23][24][25].…”

Section: Evaluation Of Immune Gene Expression By Real Time Pcrmentioning

confidence: 99%

The pyloric caeca area is a major site for IgM+ and IgT+ B cell recruitment in response to oral vaccination in rainbow trout

Ballesteros¹,

Saint-Jean²,

Pérez-Prieto³

et al. 2013

Fish & Shellfish Immunology

Self Cite

View full text Add to dashboard Cite

Although previous studies have characterized some aspects of the immune response of the teleost gut in response to diverse pathogens or stimuli, most studies have focused on the posterior segments exclusively. However, there are still many details of how teleost intestinal immunity is regulated that remain unsolved, including the location of IgM + and IgT + B cells along the digestive tract and their role during the course of a local stimulus. Thus, in the current work, we have studied the B cell response in five different segments of the rainbow trout (Oncorhynchus mykiss) digestive tract in both naïve fish and fish orally vaccinated with an alginate-encapsulated DNA vaccine against infectious pancreatic necrosis virus (IPNV). IgM + and IgT + cells were identified all along the tract with the exception of the stomach in naïve fish. While IgM + cells were mostly located in the lamina propria (LP), IgT + cells were primarily localized as intraepithelial lymphocytes (IELs). Scattered IgM + IELs were only detected in the pyloric caeca. In response to oral vaccination, the pyloric caeca region was the area of the digestive tract in which a major recruitment of B cells was demonstrated through both real time PCR and immunohistochemistry, observing a significant increase in the number of both IgM + and IgT + IELs. Our findings demonstrate that both IgM + and IgT + respond to oral stimulation and challenge the paradigm that teleost IELs are exclusively T cells. Unexpectedly, we have also detected B cells in the fat tissue associated to the digestive tract that respond to vaccination, suggesting that these cells surrounded by adipocytes also play a role in mucosal defense.

show abstract

Immune responses elicited in rainbow trout through the administration of infectious pancreatic necrosis virus-like particles

Cited by 22 publications

References 31 publications

Isolation and Characterization of Salmonid CD4+ T Cells

Isolation and Characterization of Salmonid CD4+ T Cells

Differential Modulation of IgT and IgM upon Parasitic, Bacterial, Viral, and Dietary Challenges in a Perciform Fish

The pyloric caeca area is a major site for IgM+ and IgT+ B cell recruitment in response to oral vaccination in rainbow trout

Contact Info

Product

Resources

About