Immune responses elicited by vaccinations with Candida albicans ribosomes in cyclophosphamide treated animals

Abstract

This study describes humoral and cell mediated immune (CMI) responses detected in cyclophosphamide (CY) treated animals who were vaccinated with Candida albicans ribosomes and were protected against systemic candidiasis (previous study). Mice treated with CY and vaccinated with C. albicans ribosomes revealed CMI responses towards the ribosomes as measured in vivo by the foot pad swelling test and in vitro by the lymphocyte transformation assay. Both reactions were higher in CY treated and ribosome vaccinated mice than in controls (mice that were only vaccinated). Humoral immune responses were measured by the enzyme linked immunosorbent assay (ELISA). Anti ribosomal antibody titer contrary to the CMI responses was lower in CY treated animals than in non treated controls. These data point to a possible explanation of the mechanisms underlying the ribosomal vaccinations in CY treated hosts, and show the potential of such vaccinations in compromised individuals.

Evidence for macrophage-mediated protection against lethal Candida albicans infection

“…There are several reports in the literature of acquired resistance to C. albicans induced by viable inocula (9,11,26) or ribosomes (31). In most of these studies, however, no data were presented on the immune status and in particular on natural cell-mediated reactions of the animals at the time of challenge.…”

CD4+-T-Cell-Mediated Resistance to Systemic Murine Candidiasis Induced by a Membrane Fraction ofCandida albicans

“…Antigens that induce resistance to systemic candidiasis include cell wall components and cytosol-soluble proteins (5,9,17). In the present study, we searched subcellular fractions of Candida cells for antigens using mice that had acquired high resistance to systemic candidiasis by immunization with two s.c. injections of live cells and IFA.…”

Activity of an Intralipid formulation of nystatin in murine systemic candidiasis

“…A transiently compromised state was induced as described previously [14] by intraperitoneal injection of the immunosuppressive agent cyclophosphamide (CYC) (Sigma-Aldrich, St Louis, MO) at a dose of 200 mg/kg 3 days prior to infection. At this time point, mice were in an immunocompromised state, as previously described, demonstrating a decrease in the number of white blood cells and a reduction in body weight [15].…”