Immune responses and intestinal morphology of striped catfish, <i>Pangasianodon hypophthalmus</i>
 (Sauvage, 1878), fed dietary nucleotides

Yaghobi, Maryam; Dorafshan, Salar; Akhlaghi, Mostafa; Heyrati, Fatemeh Paykan; Mahmoudi, Nemat

doi:10.1111/jai.12489

Cited by 11 publications

(5 citation statements)

References 19 publications

(28 reference statements)

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“…observations of the present study were marginally comparable with the earlier report of 90-134 mg dl À1 glucose and 84.1 ± 14.1 ng ml À1 cortisol level in P. hypophthalmus byYaghobi et al (2015) andNguyen et al (2021), respectively. The SOD assay and CAT are oxidative enzymes that influence the homeostasis in cells and are the key factors in animal defence against oxidative stress(Nuwansi et al, 2021).…”

supporting

confidence: 91%

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Effect of potassium supplementation on osmoregulatory and stress response of Pangasianodon hypophthalmus (Sauvage, 1878) with Spinacia oleraceaL. in aquaponics

John

Verma

Krishnani

et al. 2022

Journal of Fish Biology

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This study evaluated the effect of different potassium supplementation dosages on the physiological responses of Pangasianodon hypophthalmus reared in an aquaponic system with Spinacia oleracea L. for 60 days. The system comprised of a rectangular fish tank of 168 l capacity (water volume = 100 l) with nutrient film technique (NFT)–based hydroponic component with fish to plant ratio of 2.8 kg m−3: 28 plants m−2 in all the treatments. The osmoregulatory and stress parameters of P. hypophthalmus at four different potassium dosages of T1 (90 mg l−1), T2 (120 mg l−1), T3 (150 mg l−1) and T4 (180 mg l−1) were compared with C (control, 0 mg l−1) to examine the potassium level to be applied to aquaponics. The water quality parameters and fish production were found to have no adverse impact due to potassium supplementation. The spinach yield during two harvests, i.e., before and after potassium supplementation, revealed that the yield was significantly higher (P < 0.05) after supplementation with the highest yield in T3 and T4. The osmoregulatory parameters such as plasma osmolality, Na+, K+ ATPase activity in gill and plasma ionic profile (Cl−, Ca2+ and Na+) showed an insignificant variation (P > 0.05) between control and treatments except for higher plasma potassium concentration (1.98 ± 0.19 mmol l−1) in T4. The stress and antioxidant enzyme analysis exhibited significantly higher plasma glucose and superoxide dismutase (SOD) activity in gill and liver in T4, whereas cortisol and catalase showed an insignificant difference (P > 0.05). The experimental findings demonstrated that the potassium dosage up to 150 mg l−1 could be suggested as optimum for P. hypophthalmus and spinach aquaponics without impairing the health and oxidative status of P. hypophthalmus.

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supporting

confidence: 91%

“…The observations of the present study were marginally comparable with the earlier report of 90–134 mg dl −1 glucose and 84.1 ± 14.1 ng ml −1 cortisol level in P. hypophthalmus by Yaghobi et al . (2015) and Nguyen et al . (2021), respectively.…”

Section: Discussionmentioning

confidence: 97%

Effect of potassium supplementation on osmoregulatory and stress response of Pangasianodon hypophthalmus (Sauvage, 1878) with Spinacia oleraceaL. in aquaponics

John

Verma

Krishnani

et al. 2022

Journal of Fish Biology

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“…The gastrointestinal (GI) tract in fish is a multifunctional organ, where the complex microbial community interacts with the external as well as internal environment influencing health and disease (Ringø et al 2016). Several studies have described that the gut microbiota in fish has specific metabolic, trophic, and protective functions (e.g., Yaghobi et al 2014, Liu et al 2016. Comprehensive investigations during the last two decades have the enzymatic potential of the commensal microorganisms within the GI tract of fish (Ray et al 2012, Wang et al 2018.…”

Section: Introductionmentioning

confidence: 99%

Potential gut adherent probiotic bacteria isolated from rohu, Labeo rohita (Actinopterygii: Cypriniformes: Cyprinidae): Characterisation, exo-enzyme production, pathogen inhibition, cell surface hydrophobicity, and bio-film formation

Dutta¹,

Banerjee²,

Mukherjee³

et al. 2018

Acta Ichthyol. Piscat.

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Background. Previous reports emphasized exo-enzyme production and pathogen inhibition as major criteria to select putative probiotics in carps. However, adhesion ability to the gut epithelium could be one of the decisive factors. The presently reported study was aimed to determine the probiotic potential of autochthonous bacteria isolated from gastrointestinal (GI) tract of rohu, Labeo rohita (Hamilton, 1822). Apart from characterization of functional probiotic attributes and bio-safety, the presently reported study utilized in vitro model system for preliminary selection of potentially adherent strains. Materials and methods. Altogether, 126 exo-enzyme producing bacteria were isolated from the proximal (PI) and distal (DI) segment of the GI tract and evaluated for enzyme-producing ability (viz., amylase, protease, lipase, cellulase, phytase, xylanase). Pathogen inhibition was tested by cross-streaking and double-layer method. Based on the cumulative results, isolates LR3H1A and LR3F3P were selected and identified by phenotypic and 16S rRNA partial gene sequence analyses. Both the strains were tested for their ability to grow in fish mucus, tolerance to fish bile, and bio-safety. Cell surface characteristics of the strains were analysed by aggregation assays and biofilm forming ability was determined through adherence to glass and polystyrene surfaces. Results. Seven strains (PI-4, DI-3) were primarily selected as efficient exo-enzyme producers, of which 3 strains (PI-2, DI-1) were found to be antagonistic against ≥1 of the 6 tested fish pathogens. Partial characterization of the cell-free supernatant revealed that the antagonistic compound was proteinaceous, showing its maximum efficacy at 30°C and pH 7. Isolates LR3H1A and LR3F3P were identified as Bacillus subtilis subsp. spizizenii (KF623286) and Bacillus tequilensis (KF623287), respectively. Both the strains grew well in fish mucus, tolerated diluted bile juice, and showed evidence of bio-safety. Both the strains were categorized as a moderate bio-film producer, although LR3F3P was noticed to possess stronger cell surface hydrophobicity, auto-aggregation and co-aggregation capacity than LR3H1A. Conclusion. Owing to better colonization potential, presently reported study indicated B. tequilensis LR3F3P as a putative probiotic for feed application.

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“…It has been reported Optimun® increased health and growth performance of rainbow trout (Tahmasebi-Kohyani et al, 2011, and striped bass (Li et al, 2015) at low dose administration around 0.15 % to 0.2 % in diet, while the use of higher doses was found to be effective in Black tiger shrimp (Penaeus monodon Fabricius, 1798) with the optimal dose at 0.56 % (Huu et al, 2012). The optimal dose of Optimun® for beluga sturgeon (H. huso) was found around 0.25 % to 0.35 % (Yousefi et al, 2012), while the best dose for striped catfish (Pangasianodon hypophthalmus Sauvage, 1878) was 0.25 % to 0.5 % (Yaghobi et al, 2015). The size of fish could influence the effect of dietary nucleotides.…”

Section: Discussionmentioning

confidence: 99%

“…In terms of the feeding regime implemented, both short and long periods of dietary nucleotides administrations have been reported to have positive effect on fish growth and immunity. Some experiments were conducted over short periods of time, such as 4 wk or less (Guo et al, 2017;Reda et al, 2018), but others, fed the fish with nucleotide diets for 10 wk or more (Shiau et al, 2015;Yaghobi et al, 2015). However, some researchers reported that prolonged administration of nucleotide-supplemented diets led to immune response suppression which negatively impacted the health of the fish.…”

Section: Discussionmentioning

confidence: 99%

Supplementation of Nucleotides to Enhance Performance and Immune Responses of Asian Seabass

2021

JJBS

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Supplementation of nucleotides into fish diets can be an alternative method to manage disease problems in aquaculture, since it was reported could improve the growth rate and immunity of some aquaculture species. The present study assessed the growth and immune performance of Asian seabass (Lates calcarifer Bloch 1790) after being fed diets containing different levels of a commercial nucleotide (Optimun®) and a purified mixture of nucleotides containing AMP (Adenosine monophosphate), IMP (Inosine monophosphate), UMP (Uridine monophosphate), GMP (Guanidine monophosphate) and CMP (Cytidine monophosphate) in equal amounts. Six nucleotide supplemented diets and a control diet were used in this study, namely O1 (Optimun® 0.25 %), O2 (Optimun® 0.5 %), O3 (Optimun® 0.75 %), P1 (0.25 % mixed pure nucleotides), P2 (0.5 % mixed pure nucleotides), P3 (0.75 % mixed pure nucleotide) and C (control/diet without nucleotide supplementation). The treatment diets were fed to juvenile Asian seabass (average initial weight of 13.19 g ± 0.58 g) at 3 % body weight per day for six weeks. The results showed that weight gain, total serum protein and globulin were significantly higher in fish fed diet P2 (0.5 % of a mix pure nucleotides) compared to the control group (P < 0.05). In comparison, leucocrit level and respiratory burst activity were increased significantly (P < 0.05) in fish fed diet O1 (0.25 % of Optimun®) and the highest hematocrit level (P < 0.05) occurred in diet P3 (0.75 % of mixed pure nucleotides). Nevertheless, specific growth rate, feed conversion ratio, lysozyme activity, albumin serum and survival rate were not affected by dietary nucleotides (P > 0.05). In conclusion, supplementation of nucleotides in Asian seabass diet may have positive effect on growth performance and immune response of the fish, while diet containing 0.5% of mixed pure nucleotides tend to have a better result compared to other diet groups.

show abstract

Immune responses and intestinal morphology of striped catfish, Pangasianodon hypophthalmus (Sauvage, 1878), fed dietary nucleotides

Cited by 11 publications

References 19 publications

Effect of potassium supplementation on osmoregulatory and stress response of Pangasianodon hypophthalmus (Sauvage, 1878) with Spinacia oleraceaL. in aquaponics

Effect of potassium supplementation on osmoregulatory and stress response of Pangasianodon hypophthalmus (Sauvage, 1878) with Spinacia oleraceaL. in aquaponics

Potential gut adherent probiotic bacteria isolated from rohu, Labeo rohita (Actinopterygii: Cypriniformes: Cyprinidae): Characterisation, exo-enzyme production, pathogen inhibition, cell surface hydrophobicity, and bio-film formation

Supplementation of Nucleotides to Enhance Performance and Immune Responses of Asian Seabass

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