Immune response to gut Escherichia coli and susceptibility to adjuvant arthritis in the rats

Abstract

We have investigated the humoral immune response to antigens of predominant gut aerobic bacterial strains (i.e. Escherichia coli) over the course of adjuvant arthritis and oil-induced arthritis in two inbred rat strains: Dark Agouti (DA) and Albino Oxford (AO). We report the presence of antibodies specifi c to proteins of E. coli in molecular weight range between 20-30 kDa in sera of diseased DA rats, and the absence of these antibodies in the sera of AO rats. In DA rats, CFA and IFA provoked a stronger antibody response to E. coli, especially of the IgG2b antibody class. Intramuscular administration of E. coli preceding the adjuvant arthritis induction had no effect on the development and course of disease, as well as on the activation of T cells in the draining inguinal lymph nodes. Higher serum levels of natural and induced IgA antibodies, combined with a higher CD3 + CD26 + cell percentage were found in AO rats. The observed correlation between the serologic response to commensal fl ora and rats' genetic background as a defi ning factor for arthritis susceptibility may contribute to the process of creating a favorable (or less favorable) milieu for arthritis development.

Pterostilbene exert an anti‐arthritic effect by attenuating inflammation, oxidative stress, and alteration of gut microbiota

“…Various environmental factors such as redox environment, metabolism, and transport of sulphur, arginine, iron, and zinc were modulated in the microbiota of RA patients. In this investigation, we have observed suppressed intestinal microbial diversity in CFA‐induced RA rats, which was consistent with the previous research (Kovačević‐Jovanović et al., 2015). The level of bacteroidetes anddeferribacteres was altered during the arthritis.…”

Lactobacillus rhamnosus Affects Rat Peritoneal Cavity Cell Response to Stimulation with Gut Microbiota: Focus on the Host Innate Immunity

“…Commensal bacteria were isolated from stool specimens collected from AO and DA rats, and prepared as previously described [33]. Briefly, faecal samples were cultured using different agar plates (MacConcey agar medium, Endo agar medium, Institute "Torlak," Belgrade, Serbia) under the aerobic conditions, at 37 °C for 24 h and well-isolated colonies were identified using the Gram-staining and morphological characteristics, as well as the biochemical tests and a rapid identification system (ID-32A, API Biomerieux, Marcy l'Etoile, France).…”