Immune Response to Antituberculosis Drug-Loaded Gelatin and Polyisobutyl-Cyanoacrylate Nanoparticles in Macrophages

Sarfraz, Muhammad; Shi, Wenjun; Gao, Yuan; Clas, S.‐D.; Roa, Wilson; Bou‐Chacra, Nádia Araci; Löbenberg, Raimar

doi:10.4155/tde-2015-0007

Cited by 11 publications

(9 citation statements)

References 41 publications

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“…There have also been reports of antituberculosis drugs like moxifloxacin and rifampicin being able to trigger an immune response in lung macrophages when administered after loading into gelatin and polyisobutyl cyanoacrylate nanoparticles [102].…”

Section: Application Spectrum Of Oral Nanostructures For Treatment Ofmentioning

confidence: 99%

Role of nanostructures in improvising oral medicine

Ghosh

Sil

2019

Toxicology Reports

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Section: Application Spectrum Of Oral Nanostructures For Treatment Ofmentioning

confidence: 99%

Role of nanostructures in improvising oral medicine

Ghosh

Sil

2019

Toxicology Reports

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“…The PIBCA polymer was used for nanoparticle preparation. A well-established emulsion polymerization method was used for the synthesis of PIBCA nanoparticles . In brief: 10 mL of either 0.01 M or 0.02 M hydrochloric acid was used to synthesize different particle sizes.…”

Section: Methodsmentioning

confidence: 99%

“…The continuous phase refractive index and viscosity of water was used for the measurement. At the same time, the polydispersity index (PDI) was determined to evaluate the nanoparticle size distribution . The zeta potential was measured in a clear zeta dip cell using the above-mentioned Zetasizer.…”

Section: Methodsmentioning

confidence: 99%

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Inflammation Caused by Nanosized Delivery Systems: Is There a Benefit?

et al. 2016

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Secondary macrophage cytotoxicity induced by nanoparticles was described before. The study aim was to investigate the role of secondary cytotoxic effect in a macrophage-lung cancer coculture model after nanoparticle treatment in the presence and absence of anti-inflammatory drugs. An in vitro coculture model composed of confluent alveolar macrophage MH-S and A-549 lung cancer cells separated by a 0.4 μm porous membrane was used in the study. Macrophages were treated with two sizes of gelatin nanoparticles and two sizes of poly(isobutyl cyanoacrylate) (PIBCA) nanoparticles, with and without doxorubicin as a chemotherapeutic drug. The treatment effect with and without the presence of anti-inflammatory drug was studied using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay. The model drugs were ibuprofen, celecoxib, prednisolone, dexamethasone, and methotrexate. Different nanoparticles in different sizes were synthesized with a range of physicochemical characteristics. Doxorubicin loaded nanoparticles were prepared with an entrapment efficiency of 82-83% for PIBCA and 39-42% for gelatin. Nanoparticle treatment of macrophages showed a secondary cytotoxic effect on A-549 cancer cells at 24 and 36 h, with a drop in cell viability of 40-62%. However, this effect was significantly reduced to 10-48% if the macrophages were exposed to anti-inflammatory drugs. When ibuprofen and celecoxib were used the cell viability rebounded between 24 and 36 h. For prednisolone, dexamethasone, and methotrexate the cell viability dropped further between 24 and 36 h. Macrophages exposed to nanoparticles show secondary cytotoxicity, which has a significant antitumor effect in the microclimate of the coculture model. The beneficial nanoparticle treatment effect was significantly reduced if nonsteroidal anti-inflammatory drugs (NSAIDs), glucocorticoids, or methotrexate was given at the same time. The data suggest that anti-inflammatory treatments can decrease the carrier-induced macrophage cytotoxicity and its antitumor effectiveness with chemotherapy.

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“…Debido a esta versatilidad biológica, las NMP tienen un efectivo sistema de liberación de fármacos que puede emplear diversas rutas de administración. En la Tabla 4 se aprecian reportes sobre el empleo de estas estructuras en contra de la TB (34)(35)(36)(37)(38)(39)(40) .…”

Section: Nano/micro Partículas (Nmp)unclassified

Nanopartículas como transportadores de fármacos: una herramienta prometedora contra la tuberculosis

Luna-Herrera

Pérez-Martínez

Barradas-Hernández

et al. 2021

Rev Peru Med Exp Salud Publica

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La Organización Mundial de la Salud (OMS) ubica a la tuberculosis (TB) como uno de los problemas de salud más preocupantes en la actualidad, y señala que se requieren de acciones novedosas para controlar su expansión y, de esta manera, alcanzar una de las metas establecidas en los Objetivos de Desarrollo Sostenible: reducir para 2030 la morbilidad e incidencia de TB. Para lograr este objetivo, está claro que las herramientas empleadas actualmente para su diagnóstico y tratamiento ya no son las adecuadas. En este sentido, es necesario desarrollar nuevos medicamentos y vacunas, así como novedosos procedimientos de administración de fármacos que generen una mejor respuesta, disminuyan el tiempo y optimicen los tratamientos. La nanotecnología ha incorporado en los últimos años un gran número de nuevas herramientas que incrementan considerablemente, la diversidad de mecanismos para la administración de tratamientos antituberculosos. Dicho esto, la presente revisión describe brevemente el estado actual de la farmacorresistencia en TB, así como las características generales de las nanopartículas que están evaluándose como herramientas para transportar antibióticos antituberculosos.

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Immune Response to Antituberculosis Drug-Loaded Gelatin and Polyisobutyl-Cyanoacrylate Nanoparticles in Macrophages

Abstract: NPs together with chemotherapeutic drugs might be able to trigger an immune response in macrophages. The combined effect might be able to overcome mycobacteria infections.

Cited by 11 publications

References 41 publications

Role of nanostructures in improvising oral medicine

Role of nanostructures in improvising oral medicine

Inflammation Caused by Nanosized Delivery Systems: Is There a Benefit?

Nanopartículas como transportadores de fármacos: una herramienta prometedora contra la tuberculosis

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