Immune Response Study to Live Infectious Bursal Disease  Vaccines in Broiler Chickens

Kundu, Pooja; Narang, G.; Chhabra, Rajesh; Kajal, Sushma; Mahajan, N. K.; Kakkar, Naresh

doi:10.20546/ijcmas.2017.612.307

Cited by 6 publications

(2 citation statements)

References 17 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Since IBDV adversely affects T cell function indirectly 31–33 , we evaluated both the number and function of specific T cell subsets by flow cytometry, as well as IFN-γ expression post-IBDV vaccination. Vaccination with intermediate plus IBDV vaccine has been shown to simultaneously increase the number of CD8 + T cells and decrease the number of CD4 + T cells 69,70 . In the present study, we observed a significant increase in CD4 + T cells in the combination treated birds (Fig.…”

Section: Discussionmentioning

confidence: 99%

Combination of TLR2 and TLR3 agonists derepress infectious bursal disease virus vaccine-induced immunosuppression in the chicken

Bashir

Kappala

Singh

et al. 2019

Sci Rep

View full text Add to dashboard Cite

Live intermediate plus infectious bursal disease virus (IBDV) vaccines (hot vaccines) are used for protection against the virulent IBDV strains in young chickens. We evaluated the potential of Toll-like receptor (TLR) agonists to alleviate hot vaccine-induced immunosuppression. The combination of Pam3CSK4 and poly I:C synergistically upregulated IFN-β , IFN-γ , IL-12 , IL-4 , and IL-13 transcripts and cross-inhibited IL-1β , IL-10 , and iNOS transcripts in the chicken peripheral blood mononuclear cells (PBMCs) as analyzed by quantitative real-time PCR. Further, four-week old specific pathogen free White Leghorn chickens ( n = 60) were randomly divided into six groups and either immunized with hot IBDV vaccine with or without Pam3CSK4 and/or poly I:C or not vaccinated to serve as controls. The results indicated that poly I:C alone and in combination with Pam3CSK4 alleviated vaccine-induced immunosuppression, as evidenced by greater weight gain, increased overall antibody responses to both sheep erythrocytes and live infectious bronchitis virus vaccine, upregulated IFN-γ transcripts and nitric oxide production by PBMCs ( P < 0.05), and lower bursal lesion score in the experimental birds. In conclusion, poly I:C alone and its combination with Pam3CSK4 reduced the destruction of B cells as well as bursal damage with restoration of function of T cells and macrophages when used with a hot IBDV vaccine.

show abstract

Section: Discussionmentioning

confidence: 99%

Combination of TLR2 and TLR3 agonists derepress infectious bursal disease virus vaccine-induced immunosuppression in the chicken

Bashir

Kappala

Singh

et al. 2019

Sci Rep

View full text Add to dashboard Cite

show abstract

“…It reduces in size at the age of six months, then increases at the age of 8-10months afterwards undergoes shrinkage like thymus (5). Former studies suggest that bursa is a major organ in evoking the immune system and therefore formation of antibodies (6).…”

Section: Introductionmentioning

confidence: 99%

Evaluation of Bursal Index and Bursal Lesion Scores in Broiler Flocks

Zahedi,

Jamshidi,

Poorghasemi

2023

J Hellenic Vet Med Soc

View full text Add to dashboard Cite

Background and Aims: The bursa of Fabricius (BF) is recognized as a vital organ that plays a crucial role in birds immunity. In order to determine the immunological status of the birds, this study was conducted to evaluate the bursal index, and bursal lesion scores in broilers.Materials and Methods: Therefore, a total of 200 broilers, 35-45 days of age, from 10 broiler flocks were randomly selected, weighed, then sacrificed. At necropsy, the bursa of Fabricius were obtained, examined, and weighed. Bursal index (BI) was calculated. Representative pieces of tissues from bursa of Fabricius were preserved in 10% neutral buffer formaldehyde for further histopathological evaluation.Results: The results obtained from (BI) calculated during the study period for all 22 broiler farms, revealed that more than 81% of the birds were included in bad group and less than 19% in mediocre group. Degree of microscopic lesions between different groups and even within one group varied from 0 (normal) to 5 (atrophy of BF) and included lymphoid necrosis in follicles, hyperplasia and folding of bursal inner epithelium, and formation of fibrous connective tissue within the follicles. Average bursal lesion scores for all groups under study was recorded as 2.09%.Conclusions: On the basis of all results including (BI) and bursal lesion scores it is concluded that bursal lymphoid structures suffer various degree of pathological lesions indicating suppressed immunity in broiler farms along with poor performance.

show abstract

Studies on immunopathological changes induced by commercial IBD live vaccines in poultry birds

Kajal,

Narang,

Jangir

et al. 2023

Sci Rep

Self Cite

View full text Add to dashboard Cite

Intermediate plus live strain infectious bursal disease virus (IBDV) vaccines are used to control IBDV endemic infections in India. In the present study, immunopathological changes induced by commercial infectious bursal disease live vaccines with different immunization regimes were compared. A total of days old 108 Cobb broiler chicks were randomly divided into five groups with 24 chicks each in groups I, II, III and 18 chicks each in group IV and V. Group I served as control I (no immunization) and group II and III chicks were immunized with a single dose of vaccines 1 and 2 on 17th day of age (DOA), respectively. The group IV and V chicks were immunized with vaccines 1 and 2, respectively with primary dose on 17th DOA followed by booster dose on 24th DOA. Both intermediate plus live vaccines produced gross and histopathological lesions in lymphoid organs (bursa of Fabricius, thymus, spleen and caecal tonsils). Increased CD4 + , CD8 + T cells in affected bursa of Fabricius was evidenced by immunohistochemistry. Further, up-regulation in relative mRNA expression of IFN-γ, IL-1β and IL-6 were observed in bursa of Fabricius of treated birds, with maximum alteration particularly on 14th day post single immunization and 7th day post booster immunization. The findings suggest that single immunization regime on the 17th day of age showed immunization equivalent to booster immunization with lesser lesions, therefore, may be practiced and promoted in the field conditions for the better economic returns and animal welfare.

show abstract

Immune Response Study to Live Infectious Bursal Disease Vaccines in Broiler Chickens

Cited by 6 publications

References 17 publications

Combination of TLR2 and TLR3 agonists derepress infectious bursal disease virus vaccine-induced immunosuppression in the chicken

Combination of TLR2 and TLR3 agonists derepress infectious bursal disease virus vaccine-induced immunosuppression in the chicken

Evaluation of Bursal Index and Bursal Lesion Scores in Broiler Flocks

Studies on immunopathological changes induced by commercial IBD live vaccines in poultry birds

Contact Info

Product

Resources

About