Immune response pattern of the popliteal lymph nodes of dogs with visceral leishmaniasis

Moreira, Pâmela Rodrigues Reina; Vieira, Leandro M.; Andrade, Mariana Macedo Costa de; Bandarra, Márcio de Barros; Machado, Gisele Fabrino; Munari, Danísio Prado; Vasconcelos, Rosemeri de Oliveira

doi:10.1007/s00436-010-1902-2

Cited by 22 publications

(31 citation statements)

References 19 publications

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“…The same type of granuloma was described in the popliteal lymph node. Predominance of macrophages associated with lymphoid atrophy and distortion of the popliteal architecture were evident in the symptomatic dogs (MOREIRA et al, 2010). The livers of the asymptomatic dogs presented well-formed granulomas containing T lymphocytes (SANCHEZ et al, 2004).…”

Section: Discussionmentioning

confidence: 97%

“…The dogs investigated in this study were originated from the Zoonosis Control Center in Araçatuba, (São Paulo State, Brazil), a region that is endemic for VL (MOREIRA et al, 2010;MOREIRA et al, 2013). Seventy-one Leishmania infantuminfected dogs were used, without preference for age, breed or gender.…”

Section: Methodsmentioning

confidence: 99%

“…To determine the number of immunostained cells, five microscope fields were analysed (Nikon Eclipse E200) with a 40× objective lens (MOREIRA et al, 2010(MOREIRA et al, , 2013, which presented an area of approximately 0.19625 mm 2 , considering that for the density of cells in apoptosis, were counted all cells immunostained in the field, totaling five fields observed. For amastigotes of L. infantum, were counted the presence of parasitized macrophages in each field.…”

Section: Immunohistochemical Analysismentioning

confidence: 99%

“…The splenic lymphocytes were graded thus: (0) lymphoid atrophy, (1) mild, (2) moderate or (3) severe lymphoid reactivity. Graduation of the lymphoid cells was based on the reactivity of lymphoid nodules of the white pulp, according to Moreira et al (2010), who evaluated lymphoid cells by reactivity of lymphoid nodules in the popliteal lymph nodes of dogs with Visceral Leishmaniasis.…”

Section: Histopathological Analysismentioning

confidence: 99%

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Influence of apoptosis on liver and spleen resistance in dogs with visceral leishmaniosis

Moreira

Franciscato

Rossit

et al. 2016

Rev. Bras. Parasitol. Vet.

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The aim of this study was to evaluate apoptosis and parasite load in the liver and spleen of dogs with visceral leishmaniosis (VL), using immunohistochemistry. Liver and spleen samples from 71 dogs with VL were used. The parasite load in the spleen and liver showed significant difference between organs in infected group (P=0.0219). The density of the parasite load in the spleen (median=2.4) was higher than liver (median=0.8). Immunodetection of apoptotic cells was predominant in lymphocytes and differ between the infected and control group in spleen (P=0.0307) and liver (P=0.0346). There was a significant correlation between apoptosis and parasite load (P = 0.0084; r=0.3104) only in the spleen of the infected group, where it was observed that, when increasing the number of apoptotic cells increases the parasitic load. It was concluded that the liver and spleen of infected dogs presented greater numbers of cells undergoing apoptosis (lymphocytes) than the control group, thus suggesting that this process may be contributing towards the survival of Leishmania in these organs, because lymphocyte in apoptosis did not have the ability to present and recognize the antigen, allowing the survival of the parasite.Keywords: Leishmania infantum, immune escape, apoptosis, dogs. ResumoO objetivo deste estudo foi avaliar a apoptose e a carga parasitária no fígado e baço de cães com leishmaniose visceral (LV), pela técnica de imuno-histoquímica. Foram utilizadas amostras de fígado e baço de 71 cães com LV. A carga parasitária no baço e fígado mostrou diferença significativa entre os órgãos no grupo infectado (P=0,0219). A densidade da carga de parasita no baço (média=2,4) foi maior do que no fígado (média=0,8). A imunodetecção de células em apoptose foi predominante nos linfócitos, com diferenças entre o grupo infectado e controle no baço (P=0,0307) e fígado (P=0,0346). Houve uma correlação positiva fraca entre apoptose e carga parasitária (P=0,0084; r=0,3104) apenas no baço do grupo infectado, onde observou-se que quando aumentava o número de células em apoptose aumentava a carga parasitária. Concluiu-se que o fígado e o baço de cães infectados apresentam um maior número de células que sofrem apoptose (linfócitos) do que o grupo controle, sugerindo que este processo possa contribuir para a sobrevivência de Leishmania nestes órgãos, pois os linfócitos em apoptose não tiveram a capacidade de apresentar e reconhecer o antígeno, permitindo a sobrevivência do parasita.Palavras-chave: Leishmania infantum, evasão da resposta imune, apoptose, cães.

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Section: Discussionmentioning

confidence: 97%

Section: Methodsmentioning

confidence: 99%

Section: Immunohistochemical Analysismentioning

confidence: 99%

Section: Histopathological Analysismentioning

confidence: 99%

See 2 more Smart Citations

Influence of apoptosis on liver and spleen resistance in dogs with visceral leishmaniosis

Moreira

Franciscato

Rossit

et al. 2016

Rev. Bras. Parasitol. Vet.

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“…Macrophages predominate and, in some cases, contain amastigote forms of the parasite (LONGSTAFFE; GUY, 1985;LIMA et al, 2004;GIUNCHETTI et al, 2008;MOREIRA et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

Morphological changes and parasite load of the adrenal from dogs with visceral leishmaniasis

Momo

Rocha

Moreira

et al. 2014

Rev. Bras. Parasitol. Vet.

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The objective of this study was to analyze morphological changes and parasite loads in the adrenal gland from 45 dogs with visceral leishmaniasis (VL). The animals were from the Zoonosis Control Center of Araçatuba, state of São Paulo, which is an endemic region for the disease. These animals were euthanized due to positive diagnoses of VL. The dogs were classified into asymptomatic, oligosymptomatic and symptomatic groups. The parasite load was determined by immunohistochemistry, using VL-positive dog hyperimmune serum. Nine dogs showed an inflammatory infiltrate composed, predominantly, of plasma cells and macrophages. However, only eight dogs showed macrophages with amastigote forms of the parasite, immunolabeled in the cytoplasm. The medullary and reticular layers were the most affected areas, possibly due to a favorable microenvironment created by hormones in these regions. The density of parasites in the glandular tissue was not associated with clinical signs of VL (P > 0.05). However, the presence of the parasite was always associated with the presence of a granulomatous inflammatory infiltrate. This gland may not be an ideal place for the parasite's multiplication, but the presence of injuries to the glandular tissue could influence the dog's immune system, thus favoring the parasite's survival in the host's different organs.Keywords: Leishmania infantum chagasi, adrenalitis, dog, immunohistochemistry. ResumoO objetivo deste estudo foi analisar as alterações morfológicas e a carga parasitária da glândula adrenal de 45 cães com leishmaniose visceral (LV). Os animais eram provenientes do Centro de Controle de Zoonoses (CCZ) de Araçatuba (SP), região endêmica para a doença. Esses animais são submetidos à eutanásia, devido ao diagnóstico positivo para LV. Os cães foram classificados nos grupos assintomático, oligossintomático e sintomático. A determinação da carga parasitária foi feita por imuno-histoquímica, com utilização de soro hiperimune de cão positivo para LV. Em nove cães, verificou-se um infiltrado inflamatório, composto predominantemente por plasmócitos e macrófagos. Entretanto, apenas oito cães apresentaram macrófagos com formas amastigotas do parasito imunomarcadas em seu citoplasma. As camadas medular e reticulada foram as mais afetadas, possivelmente por um microambiente favorável criado pelos hormônios nestas regiões. A densidade de parasitos no tecido glandular não foi relacionada com os sinais clínicos de LV (P > 0,05). No entanto, a presença do parasito sempre esteve associada à presença de infiltrado inflamatório granulomatoso. Possivelmente, essa glândula não é um sítio ideal para a multiplicação do protozoário, mas a presença de injúrias no tecido glandular poderia influenciar o sistema imune do cão, favorecendo a sobrevivência do parasito nos diferentes órgãos do hospedeiro.Palavras-chave: Leishmania infantum chagasi, adrenalite, cão, imuno-histoquímica.

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Lymph Nodes

Comazzi¹,

Aresu²,

Burton³

et al. 2020

Veterinary Cytology

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Immune response pattern of the popliteal lymph nodes of dogs with visceral leishmaniasis

Cited by 22 publications

References 19 publications

Influence of apoptosis on liver and spleen resistance in dogs with visceral leishmaniosis

Influence of apoptosis on liver and spleen resistance in dogs with visceral leishmaniosis

Morphological changes and parasite load of the adrenal from dogs with visceral leishmaniasis

Lymph Nodes

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