Immune response of turbot, Psetta maxima (L.) (Pisces: Teleostei), to formalin-killed scuticociliates (Ciliophora) and adjuvanted formulations

Sitjà‐Bobadilla, Ariadna; Palenzuela, Oswaldo; Álvarez-Pellitero, Pilar

doi:10.1016/j.fsi.2007.06.007

Cited by 53 publications

(35 citation statements)

References 40 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Promising protection results have been recently reported in small-scale (Sitjà-Bobadilla et al 2008) or medium-scale ) vaccination experiments using Montanide ISA 763, and data of the current work suggest the potential of GER or other complex adjuvants combining easiness of delivery (oilin-water emulsions or stabilized-lipid microparticle suspensions) and immunostimulating compounds. Thus, further studies should address large-scale experiments to compare formulations, insisting on optimum adjuvants, dosages, and polyvalent antigens (including A and D isolates), in order to obtain a combined effect on different immune response pathways without induction of tolerance.…”

Section: Discussionsupporting

confidence: 64%

“…According to Alishahi & Buchmann (2006), other immune effectors could be involved in the response and protection against I. multifilis, such as lysozyme (whose levels rise after immunization) or other non-specific elements. In a previous short-scale experiment (to evaluate the effect of the same V3 antigen and the adjuvant Montanide ISA 763 on turbot) we demonstrated the stimulation of lysozyme after immunization (Sitjà-Bobadilla et al 2008), although a significant increase was detected only after challenge, when complement levels were also increased in all groups. In the same experiment, preliminary information on the effect of GER adjuvant on turbot was also obtained, and high peroxidase levels observed in fish inoculated with V3 antigen + GER indicated a profound effect of this adjuvant in turbot immune response, which is confirmed by the high antibody levels obtained in the current study.…”

mentioning

confidence: 74%

“…The GERBU preparations used in the present study belong to this group. The presence of specific antibodies against scuticociliates has been demonstrated in turbot both in natural infections (Iglesias et al 2003) and after inoculation of different vaccine preparations in small-scale (Iglesias et al 2003, Sitjà-Bobadilla et al 2008 or medium-scale experiments. However, no information is available on the production and kinetics of specific antibodies under different antigen-formulations and/or immunization regimes in medium or large-scale long-term experiments.…”

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

“…Available information from small, laboratory-scale trials suggests partial protection against a challenge using formalin-killed vaccines in turbot (Iglesias et al 2003, Sitjà-Bobadilla et al 2008) and olive flounder (Jung et al 2004). A recent report claimed quite good turbot protection using a killed adjuvanted vaccine .…”

Section: Introductionmentioning

confidence: 99%

“…An enzymelinked immunosorbent assay (ELISA) was developed and optimized to determine the presence of specific antibodies to the ciliate. The procedures for antigen preparation and for the application of the assay are fully described by Sitjà-Bobadilla et al (2008). Briefly, whole cell lysates of ciliates from the different in vitro cultured isolates were used as antigens.…”

mentioning

confidence: 99%

See 4 more Smart Citations

Antibody responses of turbot Psetta maxima against various antigen formulations of scuticociliates Ciliophora

Palenzuela¹,

Sitjà‐Bobadilla²,

Riaza³

et al. 2009

Dis. Aquat. Org.

View full text Add to dashboard Cite

The kinetics of the antibody production and the protection at challenge were studied in turbot inoculated with various scuticociliate antigen preparations: live ciliates putatively attenuated through long-term in vitro culture (Trial 1) and formalin-killed ciliates without or with GERBU adjuvant in Trials 2, 3, and 4. Antigen used in killed preparations was a mixture of 3 different ciliate isolates (V3) in the case of Trials 2 and 3, whereas in Trial 4, monovalent (V1), trivalent (V3) or pentavalent (V5) antigens were used. A booster injection was administered 28 to 29 d post-priming in all trials. Fish were challenged with virulent live ciliates after the immunization protocol, testing 2 challenge times in Trial 2 (t 1 and t 2 ). No protection was obtained in Trial 1 with live ciliates, which in turn were not completely attenuated. Using killed-ciliate formulations, protection was high only in Trial 3 when a low dose (50 000 ciliates fish -1 ) was used for challenge. In Trial 1, heat-inactivated sera of antigen-inoculated fish agglutinated the homologous ciliate, although no specific antibodies were detectable by ELISA. In contrast, high specific antibody levels were detected in antigen-inoculated fish in Trials 2 and 4, and their amount increased progressively, usually peaking after challenge. No advantage was obtained from the use of V5 antigens compared to V1 or V3. No good correlation was observed in most cases between serum antibody levels and protection. Although the use of GERBU adjuvant generally increased the specific immune response, some undesired side effects indicate a need to adjust dosage and/or improve the formulation.KEY WORDS: Ciliates · Scuticociliatia · Adjuvants · Antibodies · Immunization · Turbot · Psetta maxima Resale or republication not permitted without written consent of the publisherDis Aquat Org 86: [123][124][125][126][127][128][129][130][131][132][133][134] 2009 The increasing frequency and severity of scuticociliatosis in turbot culture, with mortality reaching 60% in some infected stocks (authors' unpubl. data), has stressed the need to develop efficacious preventive and control strategies. Since no efficacious treatments are available, the implementation of immunoprophylactic measures seems an attractive option.Empirical data from field observations in affected farms indicate the acquisition of disease resistance in fish surviving scuticociliate epizootics, stimulating the search for a vaccine. Available information from small, laboratory-scale trials suggests partial protection against a challenge using formalin-killed vaccines in turbot (Iglesias et al. 2003, Sitjà-Bobadilla et al. 2008) and olive flounder (Jung et al. 2004). A recent report claimed quite good turbot protection using a killed adjuvanted vaccine . The search for adjuvants without the toxic side effects of mineral oils (Bowden et al. 2003, Singh & O'Hagan 2003 has led to a different family of products based on lipid microparticles, stabilized in aqueous phase by surfactants and sometimes in assoc...

show abstract

Section: Discussionsupporting

confidence: 64%

mentioning

confidence: 74%

Section: Resale or Republication Not Permitted Without Written Consenmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

mentioning

confidence: 99%

See 3 more Smart Citations

Antibody responses of turbot Psetta maxima against various antigen formulations of scuticociliates Ciliophora

Palenzuela¹,

Sitjà‐Bobadilla²,

Riaza³

et al. 2009

Dis. Aquat. Org.

View full text Add to dashboard Cite

show abstract

Adjuvants and Delivery Methods: Current and Novel

Dalmo

Bøgwald

Tafalla³

2016

Birkhäuser Advances in Infectious Diseases

View full text Add to dashboard Cite

Differences in the in vitro susceptibility to resveratrol and other chemical compounds among several Philasterides dicentrarchi isolates from turbot

Budiño

Pata²,

Leiro

et al. 2011

Parasitol Res

View full text Add to dashboard Cite

Philasterides dicentrarchi is a histophagous scuticociliate that causes important losses in aquaculture. Several strains that differ in morphological, genetic and serological characteristics and virulence have been isolated from outbreaks of turbot scuticociliatosis. In the present study, seven isolates of the ciliate were exposed in vitro to formalin, hydrogen peroxide and resveratrol (a phytoalexin produced by plants) in order to evaluate the susceptibility of the isolates to the different compounds. The LD50 values for the three compounds tested varied widely among the isolates. The LD100 values were similar among isolates for formalin (25-30 ppm) and resveratrol (60-70 ppm) but were very different for hydrogen peroxide (25->80 ppm). The results indicate that there are many physiological differences among isolates and even among specimens of the same isolates, which must be taken into account in designing control programmes. The naturally occurring resveratrol may be a good alternative to other compounds for reducing the amounts of viable ciliates in water.

show abstract

Immune response of turbot, Psetta maxima (L.) (Pisces: Teleostei), to formalin-killed scuticociliates (Ciliophora) and adjuvanted formulations

Cited by 53 publications

References 40 publications

Antibody responses of turbot Psetta maxima against various antigen formulations of scuticociliates Ciliophora

Antibody responses of turbot Psetta maxima against various antigen formulations of scuticociliates Ciliophora

Adjuvants and Delivery Methods: Current and Novel

Differences in the in vitro susceptibility to resveratrol and other chemical compounds among several Philasterides dicentrarchi isolates from turbot

Contact Info

Product

Resources

About