Immune response of the mammary gland during different stages of lactation cycle in high versus low yielding Karan Fries crossbred cows

Mukherjee, Joydip; Varshney, N.K.; Chaudhury, Mandira; Mohanty, Ashok Kumar; Dang, Ajay Kumar

doi:10.1016/j.livsci.2013.03.017

Cited by 15 publications

(17 citation statements)

References 30 publications

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“…The seasonal effect is not physiological but a result of increased bacterial contamination during weather that provides better conditions for bacterial growth and increased frequency of teat damage during pasture (Reneau 1986). The cell populations in milk DLC were in agreement with the previous reports (Mukherjee & Dang 2011;Mukherjee et al 2013). On comparing the result of milk DLC, it was found that PMN and macrophage percentage were lower and lymphocyte percentages were higher in milk samples which are in agreement to the reports of Mukherjee and Dang (2011); Gargouri et al (2008).…”

Section: Discussionsupporting

confidence: 91%

“…The value of milk SCC in our study was found to be within the range as reported by Mukherjee et al (2013) in crossbred cows. In this investigation, higher milk SCC is found in elite cows compared to non-elite cows indicating more milking stress to the udder as reported by the earlier reports stating that a positive genetic correlation between selection for increased milk production and the increased rate of clinical mastitis (Emanuelson & Philipsson 1984;Owen et al 2000).…”

Section: Discussionsupporting

confidence: 89%

“…neutrophils, lymphocytes, and macrophages, was performed by the method described by Mukherjee et al (2013).…”

Section: Isolation and Culture Of Milk Leukocytesmentioning

confidence: 99%

“…All the studies stated above focused only the prevalence of mastitis with season, but the literature is scanty regarding the alteration in mammary immunity during different seasons. In vitro activity of milk leukocytes can potentially be used to evaluate the mammary immunity (Mukherjee et al 2013). Therefore, the present studies have been conducted to study the mammary immunity in terms of in vitro activity of milk leukocytes during different seasons in crossbred cows.…”

Section: Introductionmentioning

confidence: 99%

“…Therefore, the present studies have been conducted to study the mammary immunity in terms of in vitro activity of milk leukocytes during different seasons in crossbred cows. An attempt has also been made to compare the seasonal variation of mammary immunity in highand low-yielding cows as the mammary gland of high-yielding animals is under more stress (Mukherjee et al 2013). …”

Section: Introductionmentioning

confidence: 99%

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Seasonal variation inin vitroimmune activity of milk leukocytes in elite and non-elite crossbred cows of Indian sub-tropical semi-arid climate

Mukherjee¹,

Chaudhury

et al. 2015

Biological Rhythm Research

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Immunity of mammary gland in terms of in vitro activity of milk leukocytes has been evaluated during hot-humid, summer, and winter season in elite (n = 10) and non-elite (n = 10) crossbred cows. Milk samples were collected from all the cows throughout the year at 15-day interval. Milk somatic cell counts (SCC) and differential leukocyte counts (DLC) were evaluated microscopically. Milk neutrophils, macrophages, and lymphocytes were isolated and cultured in vitro. In vitro PI of milk neutrophils and macrophages was evaluated by colorimetric NBT (nitro-blue tetrazolium) reductive assay. Mitogen-induced milk lymphocyte blastogenic response was measured by colorimetric MTT (thiazolyl blue tetrazolium bromide) assay. Milk SCC was found to be significantly (p < 0.01) higher in elite cows compared to non-elite cows irrespective of season. There was significant (p < 0.05) increase in milk SCC during hot-humid season compared to winter season in both the group of the cows. There was no significant difference between group and season in terms of DLC. In vitro phagocytic index of elite cows was significantly (p < 0.01) higher than non-elite cows. The phagocytic index was significantly (p < 0.01) decreased in summer and hot-humid season compared to winter season in both the group of animals. Macrophages isolated from elite cows having significantly (p < 0.01) lower phagocytic index than non-elite cows which significantly (p < 0.01) decreased during summer and hot-humid season compared to winter. In vitro milk lymphocyte proliferative response was significantly (p < 0.01) lower in elite cows. Activity of B-lymphocytes decreased significantly (p < 0.01) during summer and hot-humid season than winter, but activity of T-lymphocytes remains unaltered during different seasons. In conclusion, the mammary immunity in terms of in vitro activity of milk leukocytes is compromised during summer and hot-humid season in elite crossbred cows; therefore, better care and management should be taken in high-yielding cows during summer and hot-humid season to minimize intramammary infections.

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Section: Discussionsupporting

confidence: 91%

Section: Discussionsupporting

confidence: 89%

“…neutrophils, lymphocytes, and macrophages, was performed by the method described by Mukherjee et al (2013).…”

Section: Isolation and Culture Of Milk Leukocytesmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%