Immune response of newborn BALB/c mice to Cryptosporidium infection

Abstract

Cryptosporidium parvum is a protozoan parasite which causes diarrheal in human and animals worldwide. Infection transmission has reported through oralfecal by infectious objects through foods and drinks. In this study we explored the immune response pathway in animal model for C. parvum to develop the new treatment way. Oocysts collected from fecal positive for C. parvum and diluted about 1:5 in sucrose solution. New born BALB/c mice (3 days) divided to 2 different groups. Control group hadn't received any oocyst, the test groups received 5 9 10 5 oocysts. 5 mice selected for each control group and 11 mice chosen for each test group. Blood collected from heart bleeds in days of 6, 9, 12 and 16. Protein concentrations determined by bio-photometer. Dot blotting used to find out total antibody concentrations oocyst antigen. Among the test and the control groups, blots appeared in test group which means antibody production, but not any blot observed in the control groups. The non-characteristic proteins in serum were measured by the biophotometer. In this study, we investigated antibody serum production against C. parvum oocysts in new born BALB/c mice. The detected antibody through dot blot technique was our aims which had conjugated to our characteristic antiserum. The recorded numbers for the controls by biophotometer related to the non characteristic proteins in serum. The results of this study can used to produce polyclonal or monoclonal antibodies against cryptosporidiosis.

Factors influencing survival of BALB/c mice and their effects on immune efficacy in an experiment of hepatitis B immunization

“…In studies about hepatitis B immunization, it is reported that different age groups of BALB/c mice were included such as age of 4-6 weeks (Granovski et al, 2017; et al, 2017), 6-8 weeks (Gui et al, 2017;Kapadia et al, 2017;Skrastina et al, 2014;Fakharzadeh et al, 2013), 8-10 weeks (McCluskie et al, 2002Abdalla et al, 2014), 11 weeks (Perez et al, 2018), or 1,3,7 days (Weeratna et al, 2001;Ahmadian et al, 2016), among which ages of 6-8 weeks were in the majority. So it is suggested that mice of a certain age group can be selected as flexible according to different experimental requirements.…”