Immune response of flounder (Paralichthys olivaceus) was associated with the concentration of inactivated Edwardsiella tarda and immersion time

Du, Yan; Tang, Xiaoqian; Sheng, Xiuzhen; Xing, Jing; Zhan, Wenbin

doi:10.1016/j.vetimm.2015.07.002

Cited by 51 publications

(19 citation statements)

References 34 publications

(35 reference statements)

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“…A similar phenomenon was observed in this work: the specific IgM antibody against HIRRV were both detected in serum and gut mucus of flounders orally vaccinated with Ll:pSLC-G. Interestingly, the significant increase of antibody level was detected earlier in mucus, which indicated that mucosal immune response was quickly evoked and produced mucosal antibodies post oral vaccination. The similar result was also found in immersion vaccination in our previous work [43]. Taken together, oral vaccine can effectively trigger the mucosal and systemic immune responses, whereas mucosal immunity seems to make a quicker response.…”

Section: Discussionsupporting

confidence: 88%

Surface display of hirame novirhabdovirus (HIRRV) G protein in Lactococcus lactis and its immune protection in flounder (Paralichthys olivaceus)

et al. 2019

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Background Hirame novirhabdovirus (HIRRV) can infect a wide range of marine and freshwater fish, causing huge economic losses to aquaculture industry. Vaccine development, especially oral vaccine, has become an effective and convenient way to control aquatic infectious diseases. HIRRV glycoprotein (G), an immunogenic viral protein is a potential vaccine candidate for prevention of the disease. Here, we aimed to construct a recombinant Lactococcus lactis strain expressing HIRRV-G on the cell surface as an oral vaccine to prevent HIRRV. Results Glycoprotein gene of HIRRV was successfully cloned and expressed in L. lactis NZ9000 in a surface-displayed form, yielding Ll:pSLC-G. An approximately 81 kDa recombinant G protein (containing LysM anchoring motif) was confirmed by SDS-PAGE, western blotting and mass spectrometry analysis. The surface-displayed G protein was also verified by immunofluorescence and flow cytometry assays. Furthermore, to evaluate the potential of Ll:pSLC-G as oral vaccine candidate, flounders were continuously fed with commercial diet pellets coated with 1.0 × 10 9 cfu/g of induced Ll:pSLC-G for 1 week. Four weeks later, booster vaccination was performed with the same procedure. Compared with the controls, Ll:pSLC-G elicited significantly higher levels of specific IgM against HIRRV in flounder gut mucus at the second week and in serum at the fourth week ( p < 0.05). Meanwhile, oral immunization with Ll:pSLC-G could provide 60.7% protection against HIRRV infection and a significantly lower virus load was detected than the controls on the third day post-challenge ( p < 0.01). Moreover, on the first day post 1-week feeding, approximately 10 4 –10 5 recombinant L. lactis cells were detected in every gram of foregut, midgut and hindgut of flounder, which were mainly localized at the bottom of gut mucus layer; and on day 21, 10 2 –10 3 L. lactis cells could still be recovered. Conclusions HIRRV-G protein was successfully expressed on the surface of L. lactis cells, which could trigger mucosal and humoral immune response of flounder and provide considerable immune protection against HIRRV. It suggests that genetically engineered L. lactis expressing G protein can be employed as a promising oral vaccine against HIRRV infection.

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Section: Discussionsupporting

confidence: 88%

Surface display of hirame novirhabdovirus (HIRRV) G protein in Lactococcus lactis and its immune protection in flounder (Paralichthys olivaceus)

et al. 2019

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“…Other immersion methods such as hyperosmotic infiltration, low‐frequency sonophoresis and puncture may be more efficacious (reviewed in Soto et al., ; Rombout & Kiron, ; Plant & LaPatra, ; Sudheesh & Cain, ). In addition, variables to increase antigen uptake, such as vaccine concentration and incubation temperature, may need to be further optimized (Du, Tang, Sheng, Xing & Zhan, ). In this study, sablefish were immersed, as recommended by the manufacturer, for 1 min to the diluted vaccine which proved to be non‐efficacious.…”

Section: Discussionmentioning

confidence: 99%

Exploring the efficacy of vaccine techniques in juvenile sablefish,Anoplopoma fimbria

et al. 2017

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Wild sablefish, Anoplopoma fimbria, are a valuable commercial species whose populations are declining. Fortunately, sablefish are excellent species for commercial aquaculture. Sablefish raised under high-density conditions in the marine environment require the use of efficacious vaccines to control disease. Sablefish impacted by disease in net pens may have poor flesh quality and high mortality during grow-out. As a result, disease can cause financial hardship for sablefish aquaculture operators.The efficacy of a multivalent vaccine preparation for sablefish, administered either by intraperitoneal (i.p.) injection or by immersion, against atypical and typical Aeromonas salmonicida, the causative agents of atypical and typical furunculosis, respectively, was examined. A. salmonicida can affect sablefish at any age and size.Consequently, an efficacious vaccine that can be appropriately and optimally administered to all life stages is desirable. Sablefish vaccinated by immersion at~1.5 or 4.5 g with a whole-cell multivalent vaccine were not protected against either typical or atypical A. salmonicida. Factors that may have contributed to the ineffectiveness of the immersion vaccine are discussed. By contrast, the relative per cent survival (RPS) or potency of the whole-cell multivalent vaccine injected i.p. in juvenile sablefish at~50 g against typical and atypical A. salmonicida was 94.3% and 81.7% respectively. The high RPS values indicated that the vaccine successfully initiated an immune response in sablefish upon a second encounter with the pathogen.

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“…In flounder, IgM has been verified to play important roles in the mucosal immunity. We have previously developed the monoclonal antibodies (McAb) of anti-serum IgM and anti-mucus IgM of flounder, and found that the IgM antibody levels in serum and mucus increased post-immunization in flounder [ 51 , 52 , 53 , 54 ]. Moreover, a secreted component-like molecule (SC) of pIgR that mediated Igs secretions was detected in skin mucus of flounder using the polyclonal antibodies against pIgR, suggesting flounder IgM involved in mucosal immunity [ 5 ].…”

Section: Discussionmentioning

confidence: 99%

“…Healthy flounder were divided into six experimental groups and transferred into tanks (50 fish/tank) that each contained 500 L of water. The bacterin of live and inactivated E. tarda was prepared as described previously [ 54 ]. Group A was infected by intraperitoneal (IP) injection with live E. tarda (0.2 mL; 1.0 × 10 7 colony-forming units (CFU)/mL in phosphate buffer saline (PBS)); group B was infected by immersion with live E. tarda (1.0 × 10 8 CFU/mL) for 60 min; group C was immunized by IP injection with formalin-killed E. tarda (0.2 mL; 1.0 × 10 8 CFU/mL); group D was vaccinated by immersion with formalin-killed E. tarda (1.0 × 10 8 CFU/mL) for 60 min; group E was injected with equal dose PBS as negative control; and group F was not treated as blank control.…”

Section: Methodsmentioning

confidence: 99%

Immunoglobulin Tau Heavy Chain (IgT) in Flounder, Paralichthys olivaceus: Molecular Cloning, Characterization, and Expression Analyses

Tang

Zhan

et al. 2016

IJMS

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Immunoglobulin tau (IgT) is a new teleost immunoglobulin isotype, and its potential function in adaptive immunity is not very clear. In the present study, the membrane-bound and secreted IgT (mIgT and sIgT) heavy chain genes were cloned for the first time and characterized in flounder (Paralichthys olivaceus), and found the nucleic acid sequence were exactly same in the Cτ1–Cτ4 constant domains of mIgT and sIgT, but different in variable regions and the C-terminus. The amino acid sequence of mIgT shared higher similarity with Bovichtus diacanthus (51.2%) and Dicentrarchus labrax (45.0%). Amino acid of flounder IgT, IgM, and IgD heavy chain was compared and the highest similarity was found between IgT Cτ1 and IgM Cμ1 (38%). In healthy flounder, the transcript levels of IgT mRNA were the highest in gill, spleen, and liver, and higher in peripheral blood leucocytes, skin, and hindgut. After infection and vaccination with Edwardsiella tarda via intraperitoneal injection and immersion, the qRT-PCR analysis demonstrated that the IgT mRNA level was significantly upregulated in all tested tissues, with similar dynamic tendency that increased firstly and then decreased, and higher in gill, skin, hindgut, liver, and stomach in immersion than in the injection group, but no significant difference existed in spleen and head kidney between immersion and injection groups. These results revealed that IgT responses could be simultaneously induced in both mucosal and systemic tissues after infection/vaccination via injection and immersion route, but IgT might play a more important role in mucosal immunity than in systemic immunity.

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Immune response of flounder (Paralichthys olivaceus) was associated with the concentration of inactivated Edwardsiella tarda and immersion time

Cited by 51 publications

References 34 publications

Surface display of hirame novirhabdovirus (HIRRV) G protein in Lactococcus lactis and its immune protection in flounder (Paralichthys olivaceus)

Surface display of hirame novirhabdovirus (HIRRV) G protein in Lactococcus lactis and its immune protection in flounder (Paralichthys olivaceus)

Exploring the efficacy of vaccine techniques in juvenile sablefish,Anoplopoma fimbria

Immunoglobulin Tau Heavy Chain (IgT) in Flounder, Paralichthys olivaceus: Molecular Cloning, Characterization, and Expression Analyses

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