Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51

Dorneles, Elaine Maria Seles; Lima, Graciela Kunrath; Teixeira-Carvalho, Andréa; Araújo, Márcio Sobreira Silva; Martins‐Filho, Olindo Assis; Sriranganathan, Nammalwar; Qublan, Hamzeh Al; Heinemann, Marcos Bryan; Lage, Andrey Pereira

doi:10.1371/journal.pone.0136696

Cited by 55 publications

(37 citation statements)

References 63 publications

(57 reference statements)

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“…This study provides original data that lays the foundation for the development of an efficacious vaccine formulation for controlling canine brucellosis. Vaccination with RB51, S19, and REV-1 is largely used in cattle and small ruminants [35,36]. However, these vaccine strains have pathogenic potential for humans and animals, they interfere with serologic diagnosis, and they are not indicated for controlling canine brucellosis [35,37].…”

Section: Discussionmentioning

confidence: 99%

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Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs

et al. 2020

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Background/Objectives Vaccination is the most important tool for controlling brucellosis, but currently there is no vaccine available for canine brucellosis, which is a zoonotic disease of worldwide distribution caused by Brucella canis. This study aimed to evaluate protection and immune response induced by Brucella ovis ΔabcBA (BoΔabcBA) encapsulated with alginate against the challenge with Brucella canis in mice and to assess the safety of this strain for dogs. Methods Intracellular growth of the vaccine strain BoΔabcBA was assessed in canine and ovine macrophages. Protection induced by BoΔabcBA against virulent Brucella canis was evaluated in the mouse model. Safety of the vaccine strain BoΔabcBA was assessed in experimentally inoculated dogs. Results Wild type B. ovis and B. canis had similar internalization and intracellular multiplication profiles in both canine and ovine macrophages. The BoΔabcBA strain had an attenuated phenotype in both canine and ovine macrophages. Immunization of BALB/c mice with alginateencapsulated BoΔabcBA (10 8 CFU) induced lymphocyte proliferation, production of IL-10 and IFN-γ, and protected against experimental challenge with B. canis. Dogs immunized with alginate-encapsulated BoΔabcBA (10 9 CFU) seroconverted, and had no hematologic, biochemical or clinical changes. Furthermore, BoΔabcBA was not detected by isolation or PCR performed using blood, semen, urine samples or vaginal swabs at any time point over the course of this study. BoΔabcBA was isolated from lymph nodes near to the site of inoculation in two dogs at 22 weeks post immunization.

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Section: Discussionmentioning

confidence: 99%

“…Protective immune response to Brucella infection is strongly associated with IFN-γ production [36,50,51]. Thus, higher production of IFN-γ by mice immunized with alginate-encapsulated BoΔabcBA contributed for developing a protective immune response against B. canis.…”

Section: Plos Onementioning

confidence: 99%

Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs

et al. 2020

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“…IFN-γ is a significant cytokine that plays a crucial role in the host immune response. Previous studies demonstrated that the number of CD4 + cells and the expression of IFN-γ increased in the early stages of B. abortus infection [7][8]. Interestingly, the level of CD4, CD8 and CD21 in the blood of cattle increased when animals received the Brucella S19 vaccine and were stimulated by the Brucella 2308 antigen.…”

Section: Introductionmentioning

confidence: 92%

“…Interestingly, the level of CD4, CD8 and CD21 in the blood of cattle increased when animals received the Brucella S19 vaccine and were stimulated by the Brucella 2308 antigen. Meanwhile, the IFN-γ expression mediated by CD4 was dramatically increased [7,8].…”

Section: Introductionmentioning

confidence: 99%

Development of an interferon-γ release assay (IGRA) for detection of Brucella abortus and clinical diagnosis of brucellosis

Feng

Zhu

Peng

et al. 2017

J Infect Dev Ctries

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Introduction: Brucellosis, caused by Brucella abortus (B. abortus), is an important zoonosis posing a great risk to both livestock and humans. Currently, most assays for clinical diagnosis of brucellosis have been developed based on serological principles; however, these assays have a number of limitations and disadvantages. Methodology: To address this concern, the aim of this study was to develop a gamma interferon (IFN-γ) release assay (IGRA) for the diagnosis of brucellosis. Towards this end, the stimulating effect induced by different somatic antigens of B. abortus on the secretion of IFN-γ was evaluated. Results: The best antigen candidate, B. abortus strain 2308, able to induce high levels of IFN-γ expression in peripheral blood (PB) cells from cattle, was used for the development of the IGRA. The optimal concentration for stimulation was determined as 1.0×107 CFU/mL. This study demonstrated that IFN-γ was detectable on day 5 post infection (p.i.) and peaked on day 14 p.i.. Finally, the IGRA developed was used for detection of B. abortus in clinical samples, and a higher level of IFN-γ was detected in Brucella-infected samples compared to vaccination samples and negative controls. Conclusions: The optimal somatic antigen for B. abortus was identified and used to establish a robust IGRA. The IGRA developed is suitable for clinical diagnosis of brucellosis, especially in the early stages of infection.

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“…CD4 + T cells can activate the bactericidal functions of macrophages through secretion of IFN-g [21]. Dorneles et al [22] have noted that after vaccinating cattle with Brucella, CD4 + Th1 cells, induced by IFN-g, participate in the initiation and development of control of Brucella, whereas CD8 + T cells, B cells, and Th2 cells exert no significant effects [23]. IFN-g secreted from Th1 cells plays an important role in the control of brucellosis.…”

Section: Th1 Cells/th2 Cells and Brucellamentioning

confidence: 99%

Research progress on the role of immune cells in Brucella infection

Zhang¹

2018

Infection International

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Brucellosis is one of the most prevalent zoonoses in the world. Incidence of the disease has increased significantly in recent years and has seriously affected the health of human beings and the development of animal husbandry. The pathogenesis of brucellosis remains unclear. Current studies suggest that this disease may be related to changes in natural killer cells, dendritic cells, and macrophages in immune cell subsets. Brucellosis may be also related to T helper (Th) 1 cell/Th2 cell imbalance in the CD4 + T cell subset, immunoregulation of regulatory T cells and Th17 cells, and the mechanism of action of CD8 + T cell. This paper aims to review the research progress on these inherent immune cells, the CD4 + T cell subset, and CD8 + T cells in Brucella infection.

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Immune Response of Calves Vaccinated with Brucella abortus S19 or RB51 and Revaccinated with RB51

Cited by 55 publications

References 63 publications

Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs

Brucella ovis mutant in ABC transporter protects against Brucella canis infection in mice and it is safe for dogs

Development of an interferon-γ release assay (IGRA) for detection of Brucella abortus and clinical diagnosis of brucellosis

Research progress on the role of immune cells in Brucella infection

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