Immune response mechanism of mouse monocytes/macrophages treated with κ-carrageenan polysaccharide

Shu, Yang; Liu, Xue-bing; Ma, Xin-hui; Gao, Jian; He, Wei; Cao, Xian Ying; Chen, Jian

doi:10.1016/j.etap.2017.06.010

Cited by 33 publications

(15 citation statements)

References 15 publications

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“…Several studies related to the development of antiinflammatory drugs were based on the use of Cg-induced inflammation as experimental models of inflammatory diseases [56,57]. Since the proinflammatory action of Cg is mainly dependent on initial activation of macrophages that in turn produce pro-inflammatory cytokines, including TNF and IL-1β [17,18,58] the knowledge of the molecular mechanisms of Cg-activated macrophages Fig. 5 Role of eATP/P2X7R in the production of IL-1β by Cg-stimulated macrophages.…”

Section: Discussionmentioning

confidence: 99%

Molecular basis of carrageenan-induced cytokines production in macrophages

et al. 2020

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Background Low molecular weight carrageenan (Cg) is a seaweed-derived sulfated polysaccharide widely used as inflammatory stimulus in preclinical studies. However, the molecular mechanisms of Cg-induced inflammation are not fully elucidated. The present study aimed to investigate the molecular basis involved in Cg-induced macrophages activation and cytokines production. Methods Primary culture of mouse peritoneal macrophages were stimulated with Kappa Cg. The supernatant and cell lysate were used for ELISA, western blotting, immunofluorescence. Cg-induced mouse colitis was also developed. Results Here we show that Cg activates peritoneal macrophages to produce pro-inflammatory cytokines such as TNF and IL-1β. While Cg-induced TNF production/secretion depends on TLR4/MyD88 signaling, the production of pro-IL-1β relies on TLR4/TRIF/SYK/reactive oxygen species (ROS) signaling pathway. The maturation of pro-IL1β into IL-1β is dependent on canonical NLRP3 inflammasome activation via Pannexin-1/P2X7/K+ efflux signaling. In vivo, Cg-induced colitis was reduced in mice in the absence of NLRP3 inflammasome components. Conclusions In conclusion, we unravel a critical role of the NLRP3 inflammasome in Cg-induced pro-inflammatory cytokines production and colitis, which is an important discovery on the pro-inflammatory properties of this sulfated polysaccharide for pre-clinical studies. Graphical Abstract Carrageenan (Cg) is one the most used flogistic stimulus in preclinical studies. Nevertheless, the molecular basis of Cg-induced inflammation is not totally elucidated. Herein, Lopes et al. unraveled the molecular basis for Cg-induced macrophages production of biological active IL-1β. The Cg-stimulated macrophages produces pro-IL-1β depends on TLR4/TRIF/Syk/ROS, whereas its processing into mature IL-1β is dependent on the canonical NLRP3 inflammasome.

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Section: Discussionmentioning

confidence: 99%

Molecular basis of carrageenan-induced cytokines production in macrophages

et al. 2020

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“…In comparison to HMW carrageenans, in some cases, their low‐molecular weight (LMW) degradation products demonstrate increased biological activity. It was shown that in comparison to HMW carrageenans, LMW carrageenans demonstrate higher immunostimulating (Ai et al, ; Chan et al, ; Guo et al, ; Shu et al, ), antitumor (Shu et al, ; Suganya, Sanjivkumar, Chandran, Palavesam, & Immanuel, ) activity, as well as decreased cytotoxicity (Guo et al, ). This peculiarity underlines the importance of studying LMW carrageenans for their possible medical applicability.…”

Section: Introductionmentioning

confidence: 99%

In vitro antitumor and immunotropic activity of carrageenans from red algae Chondrus armatus and their low‐molecular weight degradation products

Cicinskas

Begun

Tiasto

et al. 2019

J Biomedical Materials Res

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Antitumor and immunotropic effects of κ-, λ-carrageenan from red marine algae Chondrus armatus and their low-molecular weight (LMW) degradation products were explored. Effects on human esophageal cancer cell lines KYSE30 and FLO1 viability and ability to induce production of pro-and anti-inflammatory cytokines by human monocytes was assessed. All polysaccharides demonstrated antimetabolic and cytostatic activity towards cancer lines, with high-molecular weight carrageenans possessing higher antimetabolic and lower cytostatic activity than their LMW degradation products. All carrageenans induced monocytes to produce pro-inflammatory cytokines IL1β, IL6, IL18, and TNFα. However, secretion of anti-inflammatory cytokine IL10 was induced only by LMW λ-carrageenan, which exhibited the highest cytokine production inducing efficacy overall. We demonstrate that LMW carrageenan degradation products not only retain biological activity of their precursors, but also increase their efficacy in type-dependent manner, allowing for their future development for pharmacological practice. K E Y W O R D Santitumor activity, carrageenan, Chondrus armatus, immunotropic activity

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“…It has been reported that immunoreactivity in macrophages were different between fucoidan and carrageenan. 17,38,39 Moreover, fucoidan exerted an anti-diabetic effect mediated by improving glucose intolerance and prevention of diabetes complications in animal model. 40,41 Taken together, among the exogenous sulphated polysaccharides, fucoidan may be a beneficial compound for the treatment of diabetic complications.…”

Section: Discussionmentioning

confidence: 99%

“…The concentration ranges of the sulphated polysaccharides were determined based on our previous report and other reports on the physiological effects of sulphated polysaccharides. 9,17,18 The maximum concentration of carrageenan and hyaluronic acid was 500 µg/mL because of their limited solubility in sterile water. For all polysaccharides examined, no cytotoxic effect was observed even when the concentration was increased to 500 µg/mL (fucoidan, carrageenan, hyaluronic acid) and 1000 µg/mL (HMW dextran sulphate, chondroitin sulphate, heparin) (Supplemental Figure and Supplemental Table).…”

Section: Effects Of Sulphated Polysaccharides On Toxic Age Uptake By mentioning

confidence: 99%

A comparative study of sulphated polysaccharide effects on advanced glycation end-product uptake and scavenger receptor class A level in macrophages

Nishinaka

Mori

Yamazaki

et al. 2020

Diabetes and Vascular Disease Research

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Advanced glycation end-products, especially toxic advanced glycation end-products derived from glyceraldehyde (advanced glycation end-product-2) and glycolaldehyde (advanced glycation end-product-3), are biologically reactive compounds associated with diabetic complications. We previously demonstrated that toxic advanced glycation end-products were internalised into macrophage-like RAW264.7 cells through scavenger receptor-1 class A (CD204). Toxic advanced glycation end-product uptake was inhibited by fucoidan, a sulphated polysaccharide and antagonistic ligand for scavenger receptors, suggesting that sulphated polysaccharides are emerging candidates for treatment of advanced glycation end-product–related diseases. In this study, we compared the effects of six types of sulphated and non-sulphated polysaccharides on toxic advanced glycation end-product uptake in RAW264.7 cells. Fucoidan, carrageenan and dextran sulphate attenuated toxic advanced glycation end-product uptake. Fucoidan and carrageenan inhibited advanced glycation end-product-2–induced upregulation of SR-A, while advanced glycation end-product-3–induced upregulation of scavenger receptor-1 class A was only suppressed by fucoidan. Dextran sulphate did not affect scavenger receptor-1 class A levels in toxic advanced glycation end-product–treated cells. Chondroitin sulphate, heparin and hyaluronic acid failed to attenuate toxic advanced glycation end-product uptake. Heparin and hyaluronic acid had no effect on scavenger receptor-1 class A levels, while chondroitin sulphate inhibited advanced glycation end-product-3–induced upregulation of scavenger receptor-1 class A. Taken together, fucoidan and carrageenan, but not the other sulphated polysaccharides examined, had inhibitory activities on toxic advanced glycation end-product uptake and toxic advanced glycation end-product–induced upregulation of scavenger receptor-1 class A, possibly because of structural differences among sulphated polysaccharides.

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Immune response mechanism of mouse monocytes/macrophages treated with κ-carrageenan polysaccharide

Cited by 33 publications

References 15 publications

Molecular basis of carrageenan-induced cytokines production in macrophages

Molecular basis of carrageenan-induced cytokines production in macrophages

In vitro antitumor and immunotropic activity of carrageenans from red algae Chondrus armatus and their low‐molecular weight degradation products

A comparative study of sulphated polysaccharide effects on advanced glycation end-product uptake and scavenger receptor class A level in macrophages

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