Immune response in the recombinant strain of Lagovirus europaeus GI.1a

Bębnowska, Dominika; Hrynkiewicz, Rafał; Korona-Głowniak, Izabela; Niedźwiedzka-Rystwej, Paulina

doi:10.1016/j.meegid.2023.105427

Cited by 2 publications

(1 citation statement)

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“…For example, it was previously shown that infection with different GI.1 strains results in different percentages of apoptotic granulocytes and lymphocytes, as well as different timelines of recording these changes [11][12][13]. Moreover, we have recently shown that recombinant strains also show a specific pattern of immunological parameters [14]. This indicates that features of Lagovirus europaeus/GI.1 such as antigenic variation and hemagglutination capacity influence the immune response, but it is not clear what the immune picture is after infection with strain GI.2.…”

Section: Introductionmentioning

confidence: 96%

Apoptotic Cell Death in an Animal Model of Virus-Induced Acute Liver Failure—Observations during Lagovirus europaeus/GI.2 Infection

Bębnowska,

Hrynkiewicz,

Rzeszotek

et al. 2024

IJMS

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Lagovirus europaeus/GI.2 causes severe and highly fatal Rabbit Hemorrhagic Disease (RHD). Because of its characteristics, this infection is used as an animal model for acute liver failure (ALF). Apoptosis is one of the key processes underlying ALF and has been described as one of the mechanisms of RHD pathogenesis. Apoptotic cell death has been quite well characterized in infection with different variants of GI.1 strains, but so far, the GI.2 genotype has not been widely studied. In this study, we performed an evaluation of apoptotic cell death in hepatocytes of rabbits infected with Lagovirus europaeus/GI.2. We analyzed the expression of genes involved in apoptotic cell death by real-time PCR and performed immunohistochemical (IHC) assays. We showed a significant increase in the expression of caspase-3 and the proapoptotic Bax and anti-apoptotic Bcl-2 in infected animals. In addition, we recorded increased Bax/Bcl-2 ratios. IHC analyses showed the presence of morphological signs of apoptosis in the hepatocytes of infected rabbits. Our results indicate that caspase-3 and proteins from the Bcl-2 families play a key role in apoptosis induced by Lagovirus europaeus/GI.2 infection.

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Section: Introductionmentioning

confidence: 96%

Apoptotic Cell Death in an Animal Model of Virus-Induced Acute Liver Failure—Observations during Lagovirus europaeus/GI.2 Infection

Bębnowska,

Hrynkiewicz,

Rzeszotek

et al. 2024

IJMS

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Primer Investigation of Rig-I-Like Receptors Expression in Virus Infections With the Acute Character

Lewandowski,

Hrynkiewicz,

Niedźwiedzka-Rystwej

2024

Preprint

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This study investigates the expression of RIG-I-like receptors (RLRs) – RIG-I, MDA5, and LGP2 – in Lagovirus europaeus/GI.2 infections causing Rabbit Hemorrhagic Disease. Despite over 30 years of study, the acute pathogenesis remains incompletely understood. Lack of in vitro culture systems hampers vaccine development. The experiment, conducted on European rabbits, reveals a significant increase in RLR expression during infection, particularly in organs associated with severe pathology. The findings suggest a pivotal role for RLRs, especially RIG-I, in the host's defense against Lagovirus europaeus. Further research is needed to unravel the complete molecular pathway and associated factors, offering potential insights for therapeutic interventions.

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Immune response in the recombinant strain of Lagovirus europaeus GI.1a

Cited by 2 publications

References 23 publications

Apoptotic Cell Death in an Animal Model of Virus-Induced Acute Liver Failure—Observations during Lagovirus europaeus/GI.2 Infection

Apoptotic Cell Death in an Animal Model of Virus-Induced Acute Liver Failure—Observations during Lagovirus europaeus/GI.2 Infection

Primer Investigation of Rig-I-Like Receptors Expression in Virus Infections With the Acute Character

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