Immune response in dairy cattle against combined foot and mouth disease and haemorrhagic septicemia vaccine under field conditions

Muenthaisong, Anucha; Rittipornlertrak, Amarin; Nambooppha, Boondarika; Tankaew, Pallop; Varinrak, Thanya; Pumpuang, Marutpong; Muangthai, Korkiat; Atthikanyaphak, Kheemchompu; Singhla, Tawatchai; Pringproa, Kidsadagon; Punyapornwithaya, Veerasak; Sawada, Takuo; Sthitmatee, Nattawooti

doi:10.1186/s12917-021-02889-8

Cited by 11 publications

(6 citation statements)

References 38 publications

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“…Peripheral blood mononuclear cell (PBMC) isolation was performed according to the method described in a previous study ( 39 ). Jugular or tail blood samples collected at week 10 of the experiment, that had been kept in ethylene diamine tetraacetic acid (EDTA) tubes (BD Vacutainer, Plymouth, United Kingdom), were diluted with sterilized PBS (pH 7.2; 1:1 dilution) and overlaid on 5 ml of Lymphoprep™ (STEMCELL Technologies, Vancouver, Canada) in 15 ml conical tubes.…”

Section: Methodsmentioning

confidence: 99%

Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response

et al. 2022

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Both strong innate and adaptive immune responses are an important component of protection against intraerythrocytic protozoan parasites. Resistance to bovine babesiosis is associated with interferon (IFN)-γ mediated responses. CD4+ T cells and macrophages have been identified as major effector cells mediating the clearance of pathogens. Previously, the apical membrane antigen 1 (AMA-1) was found to significantly induce the immune response inhibiting B. bovis merozoite growth and invasion. However, a detailed characterization of both humoral and cellular immune responses against the structure of B. bovis AMA-1 (BbAMA-1) has not yet been established. Herein, the present study aimed to express the recombinant BbAMA-1 domain I+II protein [rBbAMA-1(I/II)], which is the most predominant immune response region, and to characterize its immune response. As a result, cattle vaccinated with BbAMA-1(I/II) significantly developed high titters of total immunoglobulin (Ig) G antibodies and a high ratio of IgG2/IgG1 when compared to control groups. Interestingly, the BbAMA-1(I/II)-based formulations produced in our study could elicit CD4+ T cells and CD8+ T cells producing IFN-γ and tumor necrosis factor (TNF)-α. Collectively, the results indicate that immunization of cattle with BbAMA-1(I/II) could induce strong Th1 cell responses. In support of this, we observed the up-regulation of Th1 cytokine mRNA transcripts, including IFN-γ, TNF-α, Interleukin (IL)-2 and IL-12, in contrast to down regulation of IL-4, IL-6 and IL-10, which would be indicative of a Th2 cytokine response. Moreover, the up-regulation of inducible nitric oxide synthase (iNOS) was observed. In conclusion, this is the first report on the in-depth immunological characterization of the response to BbAMA-1. According to our results, BbAMA-1 is recognized as a potential candidate vaccine against B. bovis infection. As evidenced by the Th1 cell response, it could potentially provide protective immunity. However, further challenge-exposure with virulent B. bovis strain in immunized cattle would be needed to determine its protective efficacy.

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Section: Methodsmentioning

confidence: 99%

Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response

et al. 2022

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“…While there was a significant difference between the vaccinated and control groups. In this respect, Muenthaisong et al (2021) prepared and evaluated the P. multocida and foot and mouth disease combined vaccine and concluded that there was a significant difference between the combined vaccine and the P. multocida vaccine. Also, the combined vaccine can be administered safely and produce a degree of immunogenicity with no adverse effects.…”

Section: Discussionmentioning

confidence: 99%

Preparation and evaluation of a novel combined inactivated vaccine against Pasteurellosis and E. coli infection in Sheep

Ismail,

Abd El-Moneim,

Mohamed

et al. 2023

Benha Veterinary Medical Journal

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Pneumonic Pasteurellosis is a common disease in sheep that affects the sheep industry. Also, E. coli, which affects young lambs and adult sheep, causing morbidity and mortality, threatens sheep's wealth. Therefore, an inactivated adjuvanted combined vaccine against pasteurellosis and E. coli infection was prepared to counteract their catastrophic effects. In this study sheep aged 6 weeks were divided into four groups. The first group was immunized with P. multocida (A, D, B6) and M. haemolytica (A, P. trehalosi type T) vaccine. The second was immunized with the E. coli (K99) vaccine. The third was immunized with a combined P. multocida, M. haemolytica, and E. coli vaccine. The fourth was kept as a non-vaccinated control group. All sheep were injected subcutaneously with two doses (2 weeks apart) of the vaccines (1 ml/dose). The vaccination of sheep was at six weeks of age. The highest antibody titers after boostering for groups vaccinated with P. multocida and M. haemolytica vaccines were as follows: the 1 st group and the 3 rd group for type A, B6, and P. trehalosi at the 4 th month, but P. multocida type D at the 5 th month and M. haemolytica type A at the 3 rd month. The 2 nd and 3 rd groups had the highest antibody titers for E. coli at the 3 rd month. Statistically, there were no significant differences between the results of the combined or single vaccines. Briefly, the combined inactivated vaccine against pasteurellosis and E. coli has good effects, with priority given to protecting sheep against these diseases and decreasing the stress on the livestock and the efforts of vaccination.

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“…Second, FMD was not perceived as the most important livestock disease by village heads, ranked second after HS in both 2016 and 2020. Since HS was the top concern for Laotian smallholders, combined FMD and HS vaccines could be an option to incentivize smallholders to vaccinate animals for both diseases (Muenthaisong et al., 2021). For sustainable FMD control, it is important to maximize participation by smallholders in disease control (Knight‐Jones et al., 2016).…”

Section: Discussionmentioning

confidence: 99%

Socioeconomic impacts of clinical foot‐and‐mouth disease and a risk‐based partial vaccination campaign for smallholders in Lao People's Democratic Republic

Wada

Subharat

Sutar

et al. 2022

Transbounding Emerging Dis

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Foot-and-mouth disease (FMD) is endemic in Lao People's Democratic Republic (PDR).A risk-based partial vaccination campaign was implemented in Champasak, Savannakhet and Xiengkhouang Provinces in Lao PDR in 2016-2020, which had limited effects on reducing the circulation of FMD virus. The objectives of this study were to measure the socioeconomic effects of (i) a clinical FMD occurrence and (ii) the vaccination campaign on livestock production performance of smallholders in Lao PDR in 2016-2020. Two questionnaire surveys were conducted at the beginning ( 2016) and the end (2020) of the campaign, involving 1609 smallholders in 160 villages comparing areas with and without FMD control. Mixed-effect multivariable regression models were fitted to measure the association between livestock production parameters at the household level (rates of birth, sale, purchase, and death, sale price, costs, gross gains and net gains) and (i) a clinical FMD occurrence and (ii) the vaccination campaign, while adjusting for village, herd size, province, vaccination status and FMD knowledge.We found no significant difference in annual household-level costs, gains and net gains by due absence of FMD. However, smallholders with FMD had a 52% higher cattle death rate, a 43% lower goat sale rate and a 78% lower pig sale price than those without FMD. Smallholders with FMD also had a lower purchase rate of livestock (cattle −15% and pigs −93%) compared with those without FMD. There was a general improvement in livestock production in target villages over the 5 years of the vaccination campaign, whereas there was an overall decline in non-target villages, resulting in USD 218 higher net gains in target villages in 2020 compared with non-target villages, possibly due to reduced severity of disease and improved awareness on FMD and general livestock health. The findings of this study will help improve strategies for sustainable and efficient FMD control in Lao PDR, while the challenges and importance of gaining participation by smallholders are highlighted.

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Immune response in dairy cattle against combined foot and mouth disease and haemorrhagic septicemia vaccine under field conditions

Cited by 11 publications

References 38 publications

Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response

Immunization of Cattle With Recombinant Structural Ectodomains I and II of Babesia bovis Apical Membrane Antigen 1 [BbAMA-1(I/II)] Induces Strong Th1 Immune Response

Preparation and evaluation of a novel combined inactivated vaccine against Pasteurellosis and E. coli infection in Sheep

Socioeconomic impacts of clinical foot‐and‐mouth disease and a risk‐based partial vaccination campaign for smallholders in Lao People's Democratic Republic

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