Immune response and energy metabolism of Chlamys farreri under Vibrio anguillarum challenge and high temperature exposure

Wang, Xingqiang; Wang, Lingling; Zhang, Huan; Ji, Qingzhi; Song, Linsheng; Qiu, Limei; Zhou, Zhi; Wang, Mengqiang; Wang, Leilei

doi:10.1016/j.fsi.2012.08.026

Cited by 95 publications

(39 citation statements)

References 41 publications

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“…Energy metabolism is tightly related with glucose and ATP levels (Sartoris et al, 2003;Wang et al, 2012). In our study, both thermal stresses (20°C and 25°C) disturbed energy metabolism in A. japonicus muscle tissues with elevated glucose and depressed ATP.…”

Section: Discussionmentioning

confidence: 55%

Metabolomic responses of sea cucumber Apostichopus japonicus to thermal stresses

Shao

Chen

et al. 2015

Aquaculture

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Section: Discussionmentioning

confidence: 55%

Metabolomic responses of sea cucumber Apostichopus japonicus to thermal stresses

Shao

Chen

et al. 2015

Aquaculture

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“…The effect of absolute temperature, however, cannot account for the difference in mortality between stressed and acclimated oysters. Wang et al (2012) reported synergic effects of heat stress and infection on scallop survival and attributed it to energetic stress. A similar mechanism may have played a role for the oyster mortality, because only heat stress, but not cold stress has been shown to increase energy consumption in Pacific oysters (Bougrier et al, 1995;Malham et al, 2009).…”

Section: Discussionmentioning

confidence: 99%

Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection

2014

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Microbiota provide their hosts with a range of beneficial services, including defense from external pathogens. However, host-associated microbial communities themselves can act as a source of opportunistic pathogens depending on the environment. Marine poikilotherms and their microbiota are strongly influenced by temperature, but experimental studies exploring how temperature affects the interactions between both parties are rare. To assess the effects of temperature, temperature stress and infection on diversity, composition and dynamics of the hemolymph microbiota of Pacific oysters (Crassostrea gigas), we conducted an experiment in a fully-crossed, three-factorial design, in which the temperature acclimated oysters (8 or 22 1C) were exposed to temperature stress and to experimental challenge with a virulent Vibrio sp. strain. We monitored oyster survival and repeatedly collected hemolymph of dead and alive animals to determine the microbiome composition by 16s rRNA gene amplicon pyrosequencing. We found that the microbial dynamics and composition of communities in healthy animals (including infection survivors) were significantly affected by temperature and temperature stress, but not by infection. The response was mediated by changes in the incidence and abundance of operational taxonomic units (OTUs) and accompanied by little change at higher taxonomic levels, indicating dynamic stability of the hemolymph microbiome. Dead and moribund oysters, on the contrary, displayed signs of community structure disruption, characterized by very low diversity and proliferation of few OTUs. We can therefore link short-term responses of host-associated microbial communities to abiotic and biotic factors and assess the potential feedback between microbiota dynamics and host survival during disease.

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“…Wang et al found the increased amount of ROS in V. anguillarum-challenged scallop Chlamys farreri and subsequent oxidative stress, together with the immune stress indicated by increased SOD activity, up-regulated mRNA expression levels of HSP 90, HSP 70 and so on [4]. Essentially, the oxidative and immune stresses are often concurrently in marine bivalves induced by pathogens or environmental contaminants, since the generation of ROS such as free radicals can damage those molecules involved in the immune system [3,4,38]. The significantly higher levels of mRNA expression of GPx and defensin in V. anguillarum-challenged group implied that V. anguillarum could induce more severe oxidative and immune stresses than V. splendidus did in clam.…”

Section: Discussionmentioning

confidence: 99%

“…Among these vibrios, both V. anguillarum and V. splendidus are common Gram-negative, facultatively anaerobic vibrios and widely investigated in immunity studies due to their frequent occurrences in marine environment [3,4,7]. Traditional immunity studies on marine aquaculture animals infected by vibrios focus on the identification of a certain class of immune-related functional molecules involved in the immune network, which usually can provide primary but global understanding on the immune mechanisms [8].…”

Section: Introductionmentioning

confidence: 99%

Differential metabolic responses of clam Ruditapes philippinarum to Vibrio anguillarum and Vibrio splendidus challenges

Liu

Zhao

et al. 2013

Fish & Shellfish Immunology

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a b s t r a c tClam Ruditapes philippinarum is one of the important marine aquaculture species in North China. However, pathogens can often cause diseases and lead to massive mortalities and economic losses of clam. In this work, we compared the metabolic responses induced by Vibrio anguillarum and Vibrio splendidus challenges towards hepatopancreas of clam using NMR-based metabolomics. Metabolic responses suggested that both V. anguillarum and V. splendidus induced disturbances in energy metabolism and osmotic regulation, oxidative and immune stresses with different mechanisms, as indicated by correspondingly differential metabolic biomarkers (e.g., amino acids, ATP, glucose, glycogen, taurine, betaine, choline and hypotaurine) and altered mRNA expression levels of related genes including ATP synthase, ATPase, glutathione peroxidase, heat shock protein 90, defensin and lysozyme. However, V. anguillarum caused more severe oxidative and immune stresses in clam hepatopancreas than V. splendidus. Our results indicated that metabolomics could be used to elucidate the biological effects of pathogens to the marine clam R. philippinarum.

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Immune response and energy metabolism of Chlamys farreri under Vibrio anguillarum challenge and high temperature exposure

Cited by 95 publications

References 41 publications

Metabolomic responses of sea cucumber Apostichopus japonicus to thermal stresses

Metabolomic responses of sea cucumber Apostichopus japonicus to thermal stresses

Hemolymph microbiome of Pacific oysters in response to temperature, temperature stress and infection

Differential metabolic responses of clam Ruditapes philippinarum to Vibrio anguillarum and Vibrio splendidus challenges

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