Immune-related miRNA-mRNA regulation network in the livers of DHAV-3-infected ducklings

Abstract

Background: Duck hepatitis A virus type 3 (DHAV-3) is one of the most harmful pathogens in the duck industry. However, the molecular mechanism underlying DHAV-3 infection in ducklings remains poorly understood. To study the genetic regulatory network for miRNA-mRNA and the signaling pathways involved in DHAV-3 infection in ducklings, we conducted global miRNA and mRNA expression profiling of duckling liver tissues infected with lethal DHAV-3 by high-throughput sequencing. Results: We found 156 differentially expressed miRNAs (DEMs) and 7717 differentially expressed genes (DEGs) in livers of mock-infected and DHAV-3-infected duckling. A total of 19,606 miRNA-mRNA pairs with negatively correlated expression patterns were identified in miRNA-mRNA networks constructed on the basis of these DEMs and DEGs. Moreover, immune-related pathways, including the cytokine-cytokine receptor interaction, apoptosis, Toll-like receptor, Jak-STAT, and RIG-I-like receptor signaling pathway, were significantly enriched through analyzing functions of mRNAs in the network in response to DHAV-3 infection. Furthermore, apl-miR-32-5p, apl-miR-125-5p, apl-miR-128-3p, apl-miR-460-5p, and novel-m0012-3p were identified as potential regulators in the immune-related signaling pathways during DHAV-3 infection. And some host miRNAs were predicted to target the DHAV-3 genome. Conclusions: This is the first integrated analysis of miRNA and mRNA in DHAV-3-infected ducklings. The results indicated the important roles of miRNAs in regulating immune response genes and revealed the immune related miRNA-mRNA regulation network in the DHAV-3-infected duckling liver. These findings increase our knowledge of the roles of miRNAs and their target genes in DHAV-3 replication and pathogenesis. They also aid in the understanding of host-virus interactions.

Serum microRNAs targeting ACE2 and RAB14 genes distinguish asymptomatic from critical COVID-19 patients

“…39 Besides, these miR-NAs have been previously related with viral processes. For instance, miR-32-5p has a significant regulatory role in avian hepatitis A viral infection, 40 visna maedi virus, 41 and caprine arthritis encephalitis viruses, 41 while the deregulation of the expression miR-98 has been described in a mouse model of West Nile virus neuropathogenesis. 42 Apart from these two miRNAs, hsa-miR-423-3p also exhibited RAB14 as a unique target gene.…”

Tmprss2 specific miRNAs as promising regulators for SARS-CoV-2 entry checkpoint

“…In the context of miR32, studies have documented the direct inhibition of RNA virus, primate foamy retrovirus type 1 (comparable to HIV1) at the protein level with supplementary degradative function in the coding region for influenza PB1 ( Trobaugh and Klimstra, 2017 ). Moreover, miR-32 has been reported with a significant regulatory role in avian hepatitis A viral infections ( Wu et al, 2020 ). Recently, regulations through miR98 and miR214 expression in CoVs-infected hosts has been sporadically demonstrated, subsequently, are in concordance to the observations from the current study ( Leon-Icaza et al, 2019 ).…”

Integrated Analysis of microRNAs and Metabolomics in Rat’s Serum Reveals Multi-action Modes of Qingfei Paidu Decoction for COVID-19 Treatment

“…Involving regulation of immunity and in ammatory cytokines [125,126], virus infection, e.g., SARS-CoV-2, DHAV, PDCoV, RSV [125,[127][128][129][130][131] MAPK signaling pathway Involving regulation of immunity and in ammatory cytokines [132][133][134][135][136][137], virus infection, e.g., PDCoV, HIV, H1N1, SARS-CoV-2, FMDV, IAV [133][134][135][136][137][138][139][140][141][142][143][144] signaling pathways regulating pluripotency of stem cells…”