Immune-related, lectin-like receptors are differentially expressed in the myeloid and lymphoid lineages of zebrafish

Panagos, Patoula; Dobrinski, Kimberly P.; Chen, Xianghong; Grant, Amanda W.; Traver, David; Djeu, Julie Y.; Wei, Sheng; Yoder, Jeffrey A.

doi:10.1007/s00251-005-0064-3

Cited by 35 publications

(21 citation statements)

References 43 publications

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“…The inability of pre-E11 AGM cells, which have been shown to possess multilineage (including lymphoid) differentiation capacity in vitro , to engraft adult animals may thus be due to immune rejection via natural killer (NK) cells that are highly radioresistant (Waterfall et al, 1987) and efficiently destroy MHCI-deficient cells (Lanier, 2005). Surface molecules related to mammalian NK receptors are expressed in zebrafish leukocytes (Panagos et al, 2006), suggesting that improved methods to ablate host NK cells may result in improved donor cell engraftment.…”

Section: Research Articlementioning

confidence: 99%

CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis

et al. 2008

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Development of the vertebrate blood lineages is complex, with multiple waves of hematopoietic precursors arising in different embryonic locations. Monopotent, or primitive, precursors first give rise to embryonic macrophages or erythrocytes. Multipotent, or definitive, precursors are subsequently generated to produce the adult hematopoietic lineages. In both the zebrafish and the mouse, the first definitive precursors are committed erythromyeloid progenitors (EMPs) that lack lymphoid differentiation potential. We have previously shown that zebrafish EMPs arise in the posterior blood island independently from hematopoietic stem cells (HSCs). In this report, we demonstrate that a fourth wave of hematopoietic precursors arises slightly later in the zebrafish aorta/gonad/mesonephros (AGM) equivalent. We have identified and prospectively isolated these cells by CD41 (itga2b) and cmyb expression. Unlike EMPs, CD41 + AGM cells colonize the thymus to generate rag2 + T lymphocyte precursors. Timelapse imaging and lineage tracing analyses demonstrate that AGM-derived precursors use a previously undescribed migration pathway along the pronephric tubules to initiate adult hematopoiesis in the developing kidney, the teleostean equivalent of mammalian bone marrow. Finally, we have analyzed the gene expression profiles of EMPs and AGM precursors to better understand the molecular cues that pattern the first definitive hematopoietic cells in the embryo. Together, these studies suggest that expression of CD41 and cmyb marks nascent HSCs in the zebrafish AGM, and provide the means to further dissect HSC generation and function in the early vertebrate embryo.

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Section: Research Articlementioning

confidence: 99%

CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis

et al. 2008

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“…A single nitr13 and two alternatively spliced nitr14a cDNAs were amplified via 3′ RACE using nested, overlapping primers (and touch-down PCR as described (Panagos et al 2006). The sequence of the primary and nested primers for nitr13 RACE were: 5′-ATGAGAATCGTGTGGATTTCTCTCATG-3′ and 5′-GGATTTCTCTCATGCTTCTATGCAGG-3′, respectively.…”

Section: Rapid Amplification Of Cdna Ends (Race)mentioning

confidence: 99%

Evidence for a transposition event in a second NITR gene cluster in zebrafish

et al. 2008

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Novel immune-type receptors (NITRs) are immunoglobulin variable (V) domain-containing cell surface proteins that possess characteristic activating/inhibitory signaling motifs and are expressed in hematopoietic cells. NITRs are encoded by multi-gene families and have been identified in bony fish species. A single gene cluster, which encodes 36 NITRs that can be classified into 12 families, has been mapped to zebrafish chromosome 7. We report herein the presence of a second NITR gene cluster on zebrafish chromosome 14, which is comprised of three genes (nitr13, nitr14a and nitr14b) representing two additional NITR gene families. Phylogenetic analyses indicate that the V domains encoded by the nitr13 and nitr14 genes are more similar to each other than any other zebrafish NITR suggesting that these genes arose from a tandem gene duplication event. Similar analyses comparing zebrafish Nitr13 and Nitr14 to NITRs from other fish species indicate that the nitr13 and nitr14 genes are phylogenetically related to the catfish IpNITR13 and IpNITR15 genes. Sequence features of the chromosomal region encoding nitr13 suggest that this gene arose via retrotransposition.

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“…In fact, it has recently been shown that lectins like Dectin-1 (Rogers et al 2005) and DC-SIGN (Geijtenbeek et al 2003) can synergize with TLRs and function as co-receptors to modulate host responses to infections. Several C-type lectins have been identified in the zebrafish, including the mannose-binding lectins (Vitved et al 2000) and the Group II immune-related, lectin-like receptors (Illrs; Panagos et al 2006). Each of the Illrs is predicted to possess a C-type lectin-like domain, a coiled-coil domain, a transmembrane domain, and one or two ITIM or ITIM-like cytoplasmic domains (Panagos et al 2006).…”

Section: Additional Innate Immunity Receptors In Zebrafishmentioning

confidence: 99%

“…Several C-type lectins have been identified in the zebrafish, including the mannose-binding lectins (Vitved et al 2000) and the Group II immune-related, lectin-like receptors (Illrs; Panagos et al 2006). Each of the Illrs is predicted to possess a C-type lectin-like domain, a coiled-coil domain, a transmembrane domain, and one or two ITIM or ITIM-like cytoplasmic domains (Panagos et al 2006). The presence of these ITIM or ITIM-like sequences indicate a likely role in inhibiting downstream signaling; however, it has been observed that in two of these lectins, Illr3a and Illr3b, there is an arginine residue in the transmembrane domain (Panagos et al 2006).…”

Section: Additional Innate Immunity Receptors In Zebrafishmentioning

confidence: 99%

Innate Immune System of the Zebrafish, Danio rerio

Sullivan

Kim

2008

Nucleic Acids and Molecular Biology

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There has been a revolution in immunology in recent years that has transformed the paradigmatic underpinnings of vertebrate immunology to include the innate immune response. The utilization of basally diverging model systems, like the zebrafish, provides particular insight into the origins and evolution of vertebrate immunity. Investigations aimed at exposing the breadth and complexity of innate immunity using the zebrafish model system have uncovered a broad spectrum of mechanisms, both novel and conserved, that add depth to our understanding of how the immune system functions. Of particular significance is the fact that, during the first 4-6 weeks of development, the zebrafish relies upon innate immunity as its sole mechanism of defense. This unique characteristic, combined with the zebrafish model's inherent advantages including high fecundity, external development, and optical transparency during early development, make the zebrafish a particularly attractive model of study. The establishment

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Immune-related, lectin-like receptors are differentially expressed in the myeloid and lymphoid lineages of zebrafish

Cited by 35 publications

References 43 publications

CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis

CD41+ cmyb+ precursors colonize the zebrafish pronephros by a novel migration route to initiate adult hematopoiesis

Evidence for a transposition event in a second NITR gene cluster in zebrafish

Innate Immune System of the Zebrafish, Danio rerio

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