Immune reactivity of the purified hemagglutinin of measles virus

Bellini, William J.; McFarlin, Dale E.; Silver, Grace; Mingioli, Elizabeth S.; McFarland, Henry F.

doi:10.1128/iai.32.3.1051-1057.1981

Cited by 39 publications

(10 citation statements)

References 17 publications

(11 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…MV structural proteins (HA, F, M, and NC) were purified from the cell lysate by affinity chromatography with monoclonal antibodies specific to each MV protein coupled to cyanogen bromide-activated Sepharose 4B, as described earlier (1,3 Beckman Microfuge, and the supernatant was saved. Similarly, the serum and CSF samples were incubated with 0.1 ml of S. aureus in 0.3 ml of RIPA buffer-0.5% myoglobin for 1 h at 4°C and then washed twice with 1.5 ml of RIPA buffer-0.5% myoglobin centrifuged.…”

Section: Methodsmentioning

confidence: 99%

Humoral and cellular immune responses to matrix protein of measles virus in subacute sclerosing panencephalitis

Dhib‐Jalbut

McFarland

Mingioli

et al. 1988

J Virol

Self Cite

View full text Add to dashboard Cite

The immune response to matrix (M) protein of measles virus was examined in patients with subacute scierosing panencephalitis (SSPE) and controls. Antibodies specific for M and nucleocapsid (NC) proteins in 11 serum and 8 cerebrospinal fluid (CSF) samples from patients with SSPE were quantitated by enzyme-linked immunosorbent assay by using affinity-purified measles virus proteins. Geometric mean anti-NC antibody titers were higher in the serum (6.58 0.98 [mean standard deviation]) and CSF (4.38 0.74) of SSPE patients compared with controls. Anti-M antibodies were present in the serum and CSF of all SSPE samples tested but in titers lower than those of anti-NC antibodies. Geometric mean anti-M antibody titer was 3.35 0.53 in sera from patients with SSPE compared with 3.05 + 0.66 in sera from patients with other neurological diseases and 3.12 0.74 in sera from healthy individuals. Geometric mean anti-M antibody titer was 2.59 0.86 in the CSF of eight patients with SSPE compared with a mean <1.00 for patients with other neurological disease (controls). Intrathecal synthesis of anti-M or anti-NC antibodies was established in four patients with SSPE. The cellular immune responses to M, F, HA, and NC proteins were examined in four of the patients with SSPE by lymphoproliferation and were not significantly different from those in five healthy controls. The results demonstrate humoral and cellular immune responses to M protein in patients with SSPE and indicate that it is unlikely that a defect in the immune response to this virus component accounts for the disease process in the patients studied.

show abstract

Section: Methodsmentioning

confidence: 99%

Humoral and cellular immune responses to matrix protein of measles virus in subacute sclerosing panencephalitis

Dhib‐Jalbut

McFarland

Mingioli

et al. 1988

J Virol

Self Cite

View full text Add to dashboard Cite

show abstract

“…Affinity chromatography-purified MV H has previously been used for testing lymphocytes of three high-responding individuals to MV [4]. In two out of three cases, plasma membranes isolated from MV-infected cells gave a higher response than purified H and in one case the response was almost equal.…”

Section: Discussionmentioning

confidence: 99%

T‐Cell Recognition of Measles Virus Haemagglutinin Studied in a Mouse Model

et al. 1989

View full text Add to dashboard Cite

BALB/c mice were pretreated with cyclophosphamide and immunized 2 days later with inactivated, purified measles virus (MV) mixed with dimethyl dioctadecyl ammonium bromide (DDA). Seven days later, lymph nodes (LN) were removed and lymphocytes cultured in the presence of purified MV antigens. MV haemagglutinin (H) was found to be a major antigen responsible for proliferation of the lymphocytes. Incorporation of purified H into liposomes significantly enhanced the proliferative response compared with purified H alone. Response to MV nucleocapsid protein was only moderate, and insertion of this protein into liposomes did not improve the response. As an attempt to analyse T-cell epitopes of MV H, three synthetic peptides previously found to elicit a strong antibody response were used both as priming and stimulating antigens. None of the peptides was able to elicit a secondary response when MV-primed LN cells were stimulated in vitro. However, each peptide primed T cells for a secondary challenge with purified, inactivated MV, which was demonstrated by proliferation and a delayed-type hypersensitivity assay and also by transfer experiments with peptide-primed cells.

show abstract

“…Immunity against measles involves a cellular response and a humoral response (4,5,13,(43)(44)(45). It is well known that passive antibodies protect an individual against measles (1,28), and protection was also obtained after passive transfer of cellular immunity, at least in an animal model (38).…”

mentioning

confidence: 99%

Immunosorbent Assay Based on Recombinant Hemagglutinin Protein Produced in a High-Efficiency Mammalian Expression System for Surveillance of Measles Immunity

et al. 1998

View full text Add to dashboard Cite

Recombinant hemagglutinin (H) protein of the measles virus (MV) was produced in mammalian cells with a high-yield expression system based on the Semliki Forest virus replicon. Crude membrane preparations of H protein-transfected BHK-21 cells were used to coat microtiter plates to measure specific immunoglobulin G antibodies in 228 serologically defined serum samples mainly from measles late-convalescent adults. The titers by the enzyme-linked immunosorbent assay for the H protein (H-ELISA) closely correlated with neutralization test (NT) titers (R 2 = 0.66), hemagglutination inhibition test (HI) titers (R 2 = 0.64), with the titers from a certified commercial ELISA based on whole MV-infected cells (MV-ELISA;R 2 = 0.45). The correlations described above were better than those of the commercial MV-ELISA titers with the NT (R 2 = 0.52) or HI (R 2 = 0.48) titers. By using the 2nd International Standard for anti-measles serum, the detection level of the assay corresponds to 215 mIU/ml for undiluted serum, which corresponds to the estimated threshold for protective immunity. The specificity, accuracy, and positive predictive value were, in general, better for the H-ELISA than for a commercial MV-ELISA, independent of whether HI, NT, or HI and NT were used as “gold standards.” In contrast, the H-ELISA proved to be slightly less sensitive than the MV-ELISA (sensitivities, 98.6 versus 99.5%, respectively;P was not significant). The assays did not differ significantly in the number of serum samples with positive HI and NT results (n = 212) which measured false negative (H-ELISA, 2 of 212 [0.94%]; MV-ELISA, 1 of 212 [0.47%]), but the H-ELISA detected significantly more measles-susceptible individuals than the MV-ELISA (10 of 11 versus 3 of 11, respectively;P < 0.05) among the individuals whose sera had negative HI and NT results. Our data demonstrate that the H-protein preparation that we describe could be a cost-effective alternative to current whole-virus-based ELISAs for surveillance for immunity to measles and that such an assay could be more efficient in detecting susceptibility to measles. Furthermore, unlike whole MV-based antigens, H-protein would also be suitable for use in the development of a simple field test for the diagnosis of measles.

show abstract

Immune reactivity of the purified hemagglutinin of measles virus

Cited by 39 publications

References 17 publications

Humoral and cellular immune responses to matrix protein of measles virus in subacute sclerosing panencephalitis

Humoral and cellular immune responses to matrix protein of measles virus in subacute sclerosing panencephalitis

T‐Cell Recognition of Measles Virus Haemagglutinin Studied in a Mouse Model

Immunosorbent Assay Based on Recombinant Hemagglutinin Protein Produced in a High-Efficiency Mammalian Expression System for Surveillance of Measles Immunity

Contact Info

Product

Resources

About