Immune promotive effect of bioactive peptides may be mediated by regulating the expression of SOCS1/miR‑155

Abstract

The present study was designed to evaluate the effect of bioactive hepatic peptide (BHP) on the immune function of mice and to examine the mechanism mediated by the related factors cytokine suppressor of cytokine signaling 1 (SOCS1) and microRNA (miR)-155. The mice were divided into eight groups, including a normal mouse group, normal peptide groups (low-dose, mid-dose and high-dose), an immunosuppressed group, and immunosuppressed with peptide groups (low-dose, mid-dose and high-dose). The proliferative ability of splenic lymphocytes was determined in vitro using a Cell Counting kit-8 assay. Wright's staining was used to assess the phagocytic function of macrophages. Histological changes in the spleen were evaluated by hematoxylin-eosin staining. The relevant factors SOCS1/miR-155 were assessed by immunohistochemistry and reverse transcription fluorescence-quantitative polymerase chain reaction analysis. The levels of the cytokines TGF-β1, IL-10 and IL-17A were determined by enzyme-linked immunosorbent assay. First, the organ index, percentage of lymphocytes, phagocytosis experiments and splenic lymphocyte proliferation test results revealed that the immunodeficient mouse model had been successfully established. Second, compared with the control mice, the normal peptide group mice exhibited increased spleen and thymus indices, percentages of lymphocyte subsets, macrophage phagocytosis percentages, phagocytic indices, splenic lymphocyte proliferation and expression of miR-155; however, the expression of SOCS1 was decreased in the normal peptide groups to varying extents. In addition, the expression of SOCS1 was upregulated, whereas that of miR-155 was downregulated in the immunosuppressed group. Compared with the mice in the immunosuppressed group, the mice in the immunosuppressed with peptide groups had increased spleen and thymus indices, percentages of lymphocyte subsets, macrophage phagocytosis percentages, phagocytic indices, splenic lymphocyte proliferation and expression of miR-155; however, the expression of SOCS1 was decreased in the immunosuppressed with peptide groups to varying extents. Following treatment with BHP, the secretion of TGF-β1 in the spleen of the normal mice and immunosuppressed mice was significantly decreased, and the secretion of IL-10 was significantly increased. No significant difference in the expression of IL-17A was observed among the groups. In summary, BHP improved the immune function of the normal mice and immunosuppressed mice. This data provides a scientific basis for the development of bioactive peptide health products.

Effects of Shrimp Peptide Hydrolysate on Intestinal Microbiota Restoration and Immune Modulation in Cyclophosphamide-Treated Mice

“…Marine species contain physiologically bioactive components for active pharmaceutical ingredients and are an important compound for human nutrition. Marine peptides, particularly, have attracted a lot of interest because of their potential for improving health and lowering illness risk [ 22 , 23 , 24 ]. Peptides may play a significant role in the intestine by regulating digestive enzymes, stabilizing the intestinal epithelial tight junctions, and modulating dietary absorption [ 25 , 26 ].…”

“…Peptides may play a significant role in the intestine by regulating digestive enzymes, stabilizing the intestinal epithelial tight junctions, and modulating dietary absorption [ 25 , 26 ]. Peptide hydrolysate from marine sources is safe, physiologically beneficial, improves immunity, and performs a variety of functions, i.e., anticancer, antibacterial, antiviral, antihypertensive, antithrombotic, hormonal, and cholesterol-lowering effects [ 20 , 22 , 23 , 27 ]. It has been reported that shark-derived hydrolysate upregulates the cytokines production and protects intestinal epithelial [ 24 ].…”

Naringenin and cryptotanshinone shift the immune response towards Th1 and modulate T regulatory cells via JAK2/STAT3 pathway in breast cancer

“…The spleen is the principal peripheral lymphoid organ in the body and is the location of B and T cells. Lymphocyte activity and proliferation in the spleen represent its immune ability and the cellular immune function of the body [ 46 ]. Here we showed increased proliferation of splenocytes in response to treatment with naringenin and its combination with CPT, suggesting that these two compounds influence the immune system.…”

Effect of animal-sourced bioactive peptides on the in vitro development of mouse preantral follicles

“…Unless otherwise stated, all chemicals, media and supplements used in this study were purchased from Sigma Aldrich (Shanghai, China). The BAPT with the special characteristic of ubiquitin enzyme and molecular weight as 8Kd were derived from spleen of small-tailed-Han sheep and identified by Professor Su’s research group at Inner Mongolia Medical University (Hohhot, China) with the RRLC-MS method [ 22 , 50 – 52 ].…”

“…8 The fertilization rates and embryonic developmental rates in different groups. Note: Labeling with the different letter in each column indicates significant differences between different groups (P < 0.05) as 8Kd were derived from spleen of small-tailed-Han sheep and identified by Professor Su's research group at Inner Mongolia Medical University (Hohhot, China) with the RRLC-MS method [22,[50][51][52].…”