Immune Phenotype of Children with Newly Diagnosed and Gluten-Free Diet-Treated Celiac Disease

Aron, C; Vásárhelyi, Barna; Szalay, Balázs; Molnár, Kálmán; Nagy-Szakal, Dorottya; Treszl, András; Vannay, Ádám; Arató, András; Tulassay, Tivadar; Veres, Gábor

doi:10.1007/s10620-010-1363-6

Cited by 20 publications

(11 citation statements)

References 35 publications

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“…Recently, our group has shown increased mRNA and protein levels of TLR4 in the duodenal mucosa of children with newly diagnosed CD, and these levels further increased in CD patients on GFD [8]. In addition, we found a higher prevalence of TLR4-positive dendritic cells and monocytes in the peripheral blood of patients newly diagnosed with CD compared to controls [9].…”

Section: Introductionsupporting

confidence: 50%

Decreased mucosal expression of intestinal alkaline phosphatase in children with coeliac disease

et al. 2012

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A major function of the enzyme intestinal alkaline phosphatase (iAP) is the detoxification of lipopolysaccharide (LPS), the ligand of Toll-like receptor 4 (TLR4). Hence, iAP has a role in the defence of maintaining intestinal barrier integrity. As intestinal barrier integrity is impaired in coeliac disease (CD), we tested the expression and localization of iAP in duodenal mucosa specimens from children with newly diagnosed CD (n = 10), with CD on gluten-free diet (GFD) (n = 5) and compared to those from ten healthy children. The mRNA and protein expression was determined by RT-PCR and Western blot analysis, respectively. Tissue localization of iAP and TLR4 was determined by immunofluorescence staining. iAP protein expression level was significantly lower than normal in newly diagnosed CD, while it was normalised in children on GFD. iAP and TLR4 colocalized at the epithelial surface of duodenal mucosa in each group of subjects enrolled. The finding of decreased iAP protein levels in newly diagnosed CD is consistent with its role in decreased intestinal barrier integrity. The latter may be the result of decreased LPS-detoxifying ability.

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Section: Introductionsupporting

confidence: 50%

Decreased mucosal expression of intestinal alkaline phosphatase in children with coeliac disease

et al. 2012

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“…Statistically significant correlations between the densities of mucosal CD3 + with serum tTG-2 antibody levels have been established in untreated and treated CD patients, and the number of intraepithelial CD3 + cells was also associated with the villus height:crypt depth ratio ( 33 ) . Another study in newly diagnosed CD children (aged 2–5 years) has also reported increased peripheral prevalence of HLA-DR + cells, which was partially reduced after adherence to a GFD ( 19 ) . This is in agreement with our findings, pointing to an improved effect of the GFD combined with B. longum CECT 7347 intake on this lymphocyte subset.…”

Section: Discussionmentioning

confidence: 93%

Double-blind, randomised, placebo-controlled intervention trial to evaluate the effects ofBifidobacterium longumCECT 7347 in children with newly diagnosed coeliac disease

et al. 2014

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Interactions between the immune system and the intestinal microbiota may play a role in coeliac disease (CD). In the present study, the potential effects of Bifidobacterium longum CECT 7347 in children with newly diagnosed CD were evaluated. A double-blind, randomised, placebo-controlled trial was conducted in thirty-three children who received a capsule containing either B. longum CECT 7347 (10 9 colonyforming units) or placebo (excipients) daily for 3 months together with a gluten-free diet (GFD). Outcome measures (baseline and postintervention) included immune phenotype of peripheral blood cells, serum cytokine concentration, faecal secretory IgA (sIgA) content, anthropometric parameters and intestinal microbiota composition. Comparisons between the groups revealed greater height percentile increases (P¼0·048) in the B. longum CECT 7347 group than in the placebo group, as well as decreased peripheral CD3 þ T lymphocytes (P¼0·004) and slightly reduced TNF-a concentration (P¼0·067). Within-group comparisons of baseline and final values did not reveal any differences in T lymphocytes and cytokines in the placebo group, while decreased CD3 þ (P ¼ 0·013) and human leucocyte antigen (HLA)-DR þ T lymphocytes (P ¼ 0·029) and slightly reduced TNF-a concentration (P¼ 0·085) were detected in the B. longum CECT 7347 group.Comparison between the groups showed that the administration of B. longum CECT 7347 reduced the numbers of the Bacteroides fragilis group (P¼0·020) and the content of sIgA in stools (P¼ 0·011) compared with the administration of placebo. Although this is a first exploratory intervention with limitations, the findings suggest that B. longum CECT 7347 could help improve the health status of CD patients who tend to show alterations in gut microbiota composition and a biased immune response even on a GFD.

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“…Inasmuch these changes in IEL content were universally observed in every CD patient and were age-independent, IEL phenotyping emerges as a useful complementary tool in CD study. Moreover, because most of the IEL are nonclassical lymphocytes, the previously recognized involvement of innate immune responses in CD [19,20] is, herein, stressed.…”

Section: Discussionmentioning

confidence: 94%

Dynamics of Non-conventional Intraepithelial Lymphocytes—NK, NKT, and γδ T—in Celiac Disease: Relationship with Age, Diet, and Histopathology

Calleja¹,

Vivas

Santiuste³

et al. 2011

Dig Dis Sci

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Immune Phenotype of Children with Newly Diagnosed and Gluten-Free Diet-Treated Celiac Disease

Abstract: The immune phenotype in childhood CD indicates the implication of both adaptive and innate immune system. The normalization of immune abnormalities occurs on GFD, but the kinetics of this process probably differs among different cell types.

Cited by 20 publications

References 35 publications

Decreased mucosal expression of intestinal alkaline phosphatase in children with coeliac disease

Decreased mucosal expression of intestinal alkaline phosphatase in children with coeliac disease

Double-blind, randomised, placebo-controlled intervention trial to evaluate the effects ofBifidobacterium longumCECT 7347 in children with newly diagnosed coeliac disease

Dynamics of Non-conventional Intraepithelial Lymphocytes—NK, NKT, and γδ T—in Celiac Disease: Relationship with Age, Diet, and Histopathology

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