Immune modulatory function of abundant immune-related microRNAs in microvesicles from bovine colostrum

Sun, Qi; Chen, Xi; Yu, Junchuan; Zen, Ke; Zhang, Chenyu; Li, Liang

doi:10.1007/s13238-013-2119-9

Cited by 139 publications

(164 citation statements)

References 26 publications

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“…Extracellular miRNAs were identified in a broad range of biological fluids (Pegtel et al, 2011;Huang et al, 2013;Kropp et al, 2014;Baglio et al, 2015;Dismuke et al, 2015;Wu et al, 2015). Apart from plasma, circulating miRNAs in milk are also intensely studied (Izumi et al, 2012;Kuruppath et al, 2013;Sun et al, 2013;Alsaweed et al, 2016c, b, a;Benmoussa et al, 2016;Xi et al, 2016).…”

Section: Extracellular Mirnas In Mammals and Their Transfermentioning

confidence: 99%

Literature review of baseline information to support the risk assessment of RNAi‐based GM plants

Pačes

Nič²,

Novotný³

et al. 2017

EFS3

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This report is the outcome of an EFSA procurement aiming at investigating and summarising the state of knowledge on (I) the mode-of-action of dsRNA and miRNA pathways, (II) the potential for nontarget gene regulation by dsRNA-derived siRNAs or miRNAs, (III) the determination of siRNA pools in plant tissues and the importance of individual siRNAs for silencing. The report is based on a comprehensive and systematic literature search, starting with the identification and retrieval of~190,000 publications related to the research area and further filtered down with keywords to produce focused collections used for subsequent screening of titles and abstracts. The report is comprised of an (I) Introduction to the field of small RNAs, (II) a Data and Methodologies section containing strategies used for literature search and study selection, and (III) the Results of the literature review organized according to the three main procurement tasks. The outcome of the first task reviews dsRNA and miRNA pathways in mammals (including humans), birds, fish, arthropods, annelids, molluscs, nematodes, and plants. Eight taxon-dedicated chapters are based on~1,400 cumulative references chosen from~10,000 inspected titles and abstracts. We review conserved and divergent aspects of small RNA pathways and dsRNA responses in animals and plants including structure and function of key proteins as well as four basic mechanisms: genome-encoded posttranscriptional regulations (miRNA), degradation of RNAs by short interfering RNA pools generated from long dsRNA (RNAi), transcriptional silencing, and sequence-independent responses to dsRNA. The outcome of the second task focuses on base pairing between small RNAs and their target RNAs and predictability of biological effects of small RNAs in animals and plants. The outcome of the last task reviews methodology, siRNA pools, and movement of small RNAs in plants. Potential transfer of small RNAs between species and circulating miRNAs in mammals is described in the final chapter.

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Section: Extracellular Mirnas In Mammals and Their Transfermentioning

confidence: 99%

Literature review of baseline information to support the risk assessment of RNAi‐based GM plants

Pačes

Nič²,

Novotný³

et al. 2017

EFS3

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“…The constitution of miRNAs in milk varies among different species (Hata et al, 2010;Munch et al, 2013;Izumi et al, 2014), the lactation period (Chen et al, 2010), and maternal dietary manipulation (Hata et al, 2010). Moreover, some miRNAs that are abundant in milk have been evidenced for their important functional roles in various biological processes, including immune response (Kosaka et al, 2010;Sun et al, 2013), postnatal growth (Melnik et al, 2013), and lipid metabolism (Fernández-Hernando et al, 2011). Recently, miRNAs in milk have been studied as potential biomarkers for milk quality control (Chen et al, 2010).…”

Section: Introductionmentioning

confidence: 99%

“…Several extraction methods have been used in recent studies describing milk miRNAs (Izumi et al, 2013;Sun et al, 2013). Current protocols for isolation of milk miRNAs can be divided into two categories that are mainly distinguished by whether there is an exosome enrichment step.…”

Section: Introductionmentioning

confidence: 99%

“…However, the reported exosome isolation methods, for instance, ultracentrifugation, sucrose-density gradient ultracentrifugation, filtration and size-exclusion chromatography, need complicated equipment and are difficult to execute. Moreover, it has been shown that after ultracentrifugation, more than 20% of miRNAs still exist in the supernatant (Izumi et al, 2013;Sun et al, 2013). Therefore, isolating miRNAs from liquid milk whey may be applicable and reliable.…”

Section: Introductionmentioning

confidence: 99%

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Comparative studies of two methods for miRNA isolation from milk whey

Jin

Wei

Liu

et al. 2015

J. Zhejiang Univ. Sci. B

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MicroRNAs (miRNAs) from milk whey have been considered for their potential as noninvasive biomarkers for milk quality control and disease diagnosis. However, standard protocols for miRNA isolation and quantification from milk whey are not well established. The objective of this study was to compare two methods for the isolation of miRNAs from milk whey. These two methods were modified phenol-based technique (Trizol LS ® followed by phenol precipitation, the TP method) and combined phenol and column-based approach (Trizol LS ® followed by cleanup using the miRNeasy kit, the TM method). Yield and quality of RNA were rigorously measured using a NanoDrop ND-1000 spectrophotometer and then the distribution of RNA was precisely detected in a Bioanalyzer 2100 instrument by microchip gel electrophoresis. Several endogenous miRNAs (bta-miR-141, bta-miR-146a, bta-miR-148a, bta-miR-200c, bta-miR-362, and bta-miR-375) and an exogenous spike-in synthetic control miRNA (cel-miR-39) were quantified by real-time polymerase chain reaction (PCR) to examine the apparent recovery efficiency of milk whey miRNAs. Both methods could successfully isolate sufficient small RNA (<200 nt) from milk whey, and their yields were quite similar. However, the quantification results show that the total miRNA recovery efficiency by the TM method is superior to that by the TP method. The TM method performed better than the TP for recovery of milk whey miRNA due to its consistency and good repeatability in endogenous and spike-in miRNA recovery. Additionally, quantitative recovery analysis of a spike-in miRNA may be more accurate to reflect the milk whey miRNA recovery efficiency than using traditional RNA quality analysis instruments (NanoDrop or Bioanalyzer 2100).

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“…These authors found that the expression of several miRNAs is altered by a perturbed maternal diet, particularly following a high-fat intake. Furthermore, a recent study has shown that colostrum-derived miRNAs are taken up in vitro by macrophages and modulate their immune activities, such as migration and cytokine secretion (Sun et al, 2013). Collectively, although direct in vivo evidence is still lacking for the physiological relevance of milk miRNAs, accumulating reports strongly suggest their protective roles against early infections in infants.…”

Section: Exosomal Mirna-mediated Regulation Of the Immune Systemmentioning

confidence: 99%

Physiological and pathological relevance of secretory microRNAs and a perspective on their clinical application

Katsuda¹,

Ikeda²,

Yoshioka³

et al. 2013

Biological Chemistry

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MicroRNAs (miRNAs) have attracted significant attention because of their important roles in a variety of physiological and pathological processes. Recent studies have shown that many cell types secrete miRNAs by packaging them into lipid-bilayered small vesicles called exosomes. Furthermore, exosomal miRNAs travel between cells, exert their RNAi effects in the recipient cells, and play important roles in various biological processes. In this article, we will summarize and describe the latest studies on exosomal miRNAs by focusing on their roles in cancer progression, immune regulation, and tissue repair. We will also provide a perspective on the clinical applications of this research field.

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Immune modulatory function of abundant immune-related microRNAs in microvesicles from bovine colostrum

Cited by 139 publications

References 26 publications

Literature review of baseline information to support the risk assessment of RNAi‐based GM plants

Literature review of baseline information to support the risk assessment of RNAi‐based GM plants

Comparative studies of two methods for miRNA isolation from milk whey

Physiological and pathological relevance of secretory microRNAs and a perspective on their clinical application

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