Immune marker expression in 53 lymphomas of high‐grade malignancy

Pallesen, Gorm; Madsen, M.; Schifter, Søren

doi:10.1111/j.1365-2559.1983.tb02300.x

Cited by 15 publications

(3 citation statements)

References 26 publications

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“…In several previously published studies, the proportion of T-cell lymphoma varied from 3% to 23% . [9][10][11][12] In one study, however, T-cell lymphomas represented 42% of the total. …”

Section: Comparison With Recently Published Studiesmentioning

confidence: 99%

Morphologic and Immunopathologic Spectrum of Malignant Lymphoma: Review of 211 Cases

1989

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A series of 211 malignant lymphomas in adults were reviewed and classified according to the international working formulation. Of those classified, five (2.4%) were low-grade, 115 (54.5%) intermediate-grade, and 85 (40.3%) highgrade lymphomas. Immunohistologic typing of 106 lymphomas was performed using a panel of monoclonal antibodies against T-and B-cell surface antigens. This revealed 51 (48.1%) B-cell and 41 (38.7%) T-cell lymphomas with 14 (13.2%) indeterminate. These results were compared with those from several recently published studies from other countries. The proportion of aggressive lymphomas in our series appeared to be relatively high. Furthermore, immunohistologic data in our study indicated a significantly higher proportion of T-cell lymphomas as compared to other studies. Malignant lymphoma is one of the most common malignant neoplasms in Saudi Arabia.1 A few studies describing various clinical and therapeutic aspects of malignant lymphoma in Saudi Arabia have been previously reported.2-4 However, to the best of our knowledge, a detailed study outlining the histopathologic and immunohistologic characteristics of malignant lymphoma in this country has not been published.The purpose of this study was to review the morphologic and immunopathologic features of malignant lymphomas seen in our laboratories and compare our findings with those from recently published studies from centers in other countries. Materials and MethodsAll malignant lymphomas in adults diagnosed histologically between January 1984 and December 1987 were identified. Cases in which the biopsies were performed in other institutions as well as those cases in which the biopsy material was inadequate were excluded. A total of 211 cases were selected in which the biopsy was performed at King Faisal Specialist Hospital and Research Centre and the tissue obtained was adequate for histologic diagnosis.Tissues were fixed in 10% buffered formaldehyde solution and B-5 solutions, dehydrated in graded alcohols, and embedded in paraffin in a routine manner. Three-to four-micron sections were stained with hematoxylin and eosin. Special stains such as periodic acid-Schiff (PAS) and methyl green-pyronine (MGP) were used in selected cases whenever indicated. Imprint smears from the fresh tissues were made and stained by Diff-Quik stain. Appropriate pieces of tissue were snap-frozen for cryostat sections which were used for immunohistologic staining.

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“…In several previously published studies, the proportion of T-cell lymphoma varied from 3% to 23% . [9][10][11][12] In one study, however, T-cell lymphomas represented 42% of the total. …”

Section: Comparison With Recently Published Studiesmentioning

confidence: 99%

Morphologic and Immunopathologic Spectrum of Malignant Lymphoma: Review of 211 Cases

1989

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“…S ince the introduction of monoclonal antibodies, immunohistochemistry has become an important tool in research and in diagnostic pathology (Sternberger et al 1970;Poppema et al 1981Poppema et al ,1987Pallesen et al 1983;Davey et al 1990;Lennert and Feller 1992). It enables the detection of defined antigens on cryostat sections and paraffin-embedded tissues.…”

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confidence: 99%

Constant Detection of CD2, CD3, CD4, and CD5 in Fixed and Paraffin-embedded Tissue Using the Peroxidase-mediated Deposition of Biotin-Tyramide

Malisius

Merz

Heinz

et al. 1997

J Histochem Cytochem.

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SUMMARYImmunohistochemical methods are widely used for diagnostic purposes in histopathology. However, the use of most monoclonal anti-leukocyte antibodies is limited to frozen tissues. Initially, it was believed that formalin fixation in particular, which is the gold standard for morphological tissue preservation, destroys most of the antigen binding sites. In recent years, protease digestion and the introduction of microwave techniques have significantly enhanced the sensitivity of immunohistochemical techniques, and a variety of hidden antigen sites in formalin-fixed tissue have been retrieved for initially unreactive antibodies. It therefore became clear that many of the leukocyte antigens are not irreversibly destroyed but are most probably masked during the fixation process. We developed a technique combining optimized pretreatment of formalin-fixed tissue with a dramatic enhancement of the immunohistochemical sensitivity and named it the ImmunoMax method. The ImmunoMax method proves that by optimizing the technique at the following three levels it is possible to detect formalin-sensitive leukocyte antigens: (a) standard fixation of the tissue; (b) sufficient antigen unmasking; and (c) increasing the substrate turnover by multiplication of binding sites with subsequent enhancement of the immunohistochemical reaction. Using this optimized ImmunoMax method, we were able to detect CD2, CD3, CD4, and CD5 with conventional monoclonal antibodies in formalin-fixed, paraffin-embedded tissue specimens of various lymphoid tissues. (J Histochem Cytochem 45:1665-1672, 1997)

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“…In the phenotypic characterization of malignant lymphomas conducted since 1978 at Aarhus Kommunehospital (Pallesen, Madsen & Schifter 1983) we have regularly encountered cases with no firm diagnosis and where we never had any chance to obtain fresh biopsy material. In some of these cases autopsy was performed at the Institute of Pathology.…”

Section: Introductionmentioning

confidence: 99%

Leucocyte antigens in human post mortem tissues: their preservation and loss as demonstrated by monoclonal antibody immunohistological staining

Pallesen¹,

Lm²

1985

Histopathology

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The development of monoclonal antibodies and of techniques for demonstrating antigens in situ in frozen tissue sections has been responsible for remarkable progress in diagnostic histopathology. We explored the potential of these techniques when applied to post mortem tissues that were frozen at various intervals after death and stained by monoclonal antibodies using immunoperoxidase technique. The monoclonal antibodies were selected according to their reactivity with essential markers of the lymphatic system and also to their availability. Lymph nodes and splenic tissue from 30 autopsy cases were stained in addition to thymic tissue from eight deceased infants. The antigens proved to be surprisingly well preserved. Staining could be precisely evaluated with UCHT1, OKT6 and anti-Leu-7 antibodies at least 72 h after death; staining with DAKO-pan-B, DAKO-LC and anti-Leu-3a was also very reliable. Antigens expressed by T-suppressor lymphocytes and dendritic reticulum cells were less well preserved. The T8-antigen of suppressor lymphocytes was usually demonstrable in the lymph nodes but less frequently in the splenic tissues. It is concluded that most leucocyte antigens are very resistant to post mortem disintegration, and that they can be reliably interpreted by immunohistological staining using monoclonal antibodies. We therefore recommend this in autopsy specimens in cases where in-vivo examination was not feasible.

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Immune marker expression in 53 lymphomas of high‐grade malignancy

Cited by 15 publications

References 26 publications

Morphologic and Immunopathologic Spectrum of Malignant Lymphoma: Review of 211 Cases

Morphologic and Immunopathologic Spectrum of Malignant Lymphoma: Review of 211 Cases

Constant Detection of CD2, CD3, CD4, and CD5 in Fixed and Paraffin-embedded Tissue Using the Peroxidase-mediated Deposition of Biotin-Tyramide

Leucocyte antigens in human post mortem tissues: their preservation and loss as demonstrated by monoclonal antibody immunohistological staining

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