Immune‐inflammatory cells in recurrent oral ulcers (ROU)

Abstract

Abstract— Tissue lesions from eight patients with recurrent oral ulcers (ROU) were subjected to detailed immunohistopathologic studies. In five patients, a specimen of an unaffected area from the opposite site was obtained. The main inflammatory cells in situ were CD3 positive T lymphocytes, with CD4 cells forming approximately half (range 30‐60%) and CD8 cells 20% (range 10‐30%) of all cells. CD19 positive B lymphocytes formed 5‐12% of all cells. Furthermore, 45% (range 15‐65%) of all lymphoid cells had signs of previous antigenous contact and had helper/inducer CDw29 type. Suppressor/inducer CD45R cells formed only about 20% (range 7‐50%) of all cells. Although this observation suggests involvement of antigen as a causative and/or triggering stimulus, elements of a non‐specific inflammatory response were observed as well. Endogenous peroxidase‐positive neutrophils were present at the ulcer site, and were occasionally observed intravascularly and in th extracellular matrix in areas characterized by inflammatory mononuclear cell infiltrates. Although the proportion of endogenous peroxidase‐positive, recently recruited monocytes was low, CD11b and nonspecific esterase‐positive mature tissue macrophages formed about 14% (range 5‐35%) of all inflammatory cells in situ, particularly at the periphery of the lymphoid cell infiltrates. Mast cells were also observed in all samples studied, forming 2‐5% of inflammatory cells in the richly vascularized connective tissue beneath the basement membrane. In the specimens from clinically unaffected areas, inflammatory cells were rare. Our observations stress the multifaceted nature and participation of multiple effector systems in the local tissue pathogenesis of ROU.

Recurrent Aphthous Stomatitis

“…Perhaps surprisingly, there is a decrease in the number of mononuclear cells, including CD4+ and CD8+ Tlymphocytes, in the affected and non-affected oral mucosa of RAS patients (Hayrinen-Immonen et al, 1991;Pedersen et al, 1992). In the pre-ulcerative phase of RAS, there is a local mononuclear infiltrate consisting initially of large granular lymphocytes (LGL) and T4 (CD4+) helper-induced lymphocytes (Hayrinen-Immonen et al, 1991).…”

Recurrent aphthous stomatitis: An overview

“…IgG, IgM, IgA, factor XIIIa+ dendrocytes, dendritic cells are involved in RAS formation. [31][32][33][34][35][36][37][38][39] Diagnosis and Differential Diagnosis of Recurrent Aphthous Stomatitis (RAS): There is no accurate diagnostic tool to rule out RAS from other mucosal lesions which are the manifestation of systemic disorders except family history and/or, clinical history. Diagnostic design is made to see if other disorders coincide with RAS.…”

Immunoreactivity to food protein in pathogenesis of recurrent oral ulceration

“…(48), ukazuju da CD l lЬ-i nespecificni esteraza pozitivni, zreli tkivni makrofagi (engl. nonspeci.fic esterasepositive mature tissue macrophages), cine oko 14% svih inflamatomih celija u RAU lezijama, sa povisenom ucestaloscu oko periferije infiltrata limfoidnih 6elija.…”

“…Postoj i intenzivna infiltracija leukocitima, sa predominacijom limfocita u ne-ulcerativnoj regiji, gde oni nadmasuju broj neutrofila (48,75). Monociti/makrofage su takode brojni u okolnom tkivu, lateralno (Ьосnо) od ulceracije.…”

“…Limfociti u RAU lezijama su primamo Т limfociti, а samo 5-12% od svih celija u leziji su В celije ( 48). Mali procenat plazma 6e\ija i eozinofila se moie pronaci mnogo ces6e и starijim lezijama (51 ).…”