Immune gene expression and genome-wide association analysis in rainbow trout with different resistance to Yersinia ruckeri infection

Abstract

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Whole-genome association study searching for QTL for Aeromonas salmonicida resistance in rainbow trout

“…The early infection (3 dpc) triggered a strong transcriptional up-regulation of many immune genes in different organs including those encoding pro-inflammatory cytokines IL-1β, IL-6 and TNFα and other cytokines involved in the development of adaptive immunity (IL-8, IL-10, IL-22, IL-17C2, INFγ). The clear up-regulation of previously mentioned immune markers is consistent with earlier studies in trout exposed to A. salmonicida 47,52,53 and other bacterial pathogens 12,35 . Along with the inflammation induced we saw a marked up-regulation of SAA, C3 and several other components of the innate immune system (lysozyme and cathelicidins).…”

“…The A. salmonicida infection level was monitored by quantifying the bacterial load in different organs using qPCR primers and probes targeting the aopO gene (DQ386862) of A. salmonicida 57 . The bacterial transcript level was estimated as 10 7 × 2 −∆Cq based on bacterial cDNA as previously described for Yersinia ruckeri 35 . It should be noted that dead or inactive bacteria is not detected by this method.…”

“…The genes investigated by qPCR analysis were encoding immune-relevant molecules and included SAA, C3, lysozyme, cathelicidins, IFNγ, IgM, IgT, IgD, IL-1β, IL-2, IL-4/13a, IL-8, IL-10, IL-12, IL-17A/F2, IL-17C1, IL-17C2, IL-22, TCRβ, TGFβ, TNFα. Primers and Taq-Man probes applied are listed in Supplementary TableS2and the gene expression analysis was performed as previously described35 . In brief, samples from gills, liver and spleen were homogenized (Tissue-lyser II, Qiagen, Vedbaek, Denmark) and RNA was extracted by GenEluteTM mammalian RNA kit (RTN350, Sigma-Aldrich).…”

Innate and early immune responses to Yersinia ruckeri in the spleen of rainbow trout (Oncorhynchus mykiss)

“…ERM is a serious disease that impacts global salmonid populations [12]. While some studies have begun to characterize rainbow trout immune responses to Y. ruckeri infection [6,13], no systematic transcriptomic analyses of these responses have been conducted to date. The spleen plays central roles in orchestrating innate and adaptive immune responses in sh.…”

“…Similarly, these pro-in ammatory cytokines were also upregulated in the spleen of the vaccinated rainbow trout following Y. ruckeri challenge, albeit to a lesser extent than in naïve sh [4]. The spleen is a key secondary lymphoid organ that is thus closely associated with rainbow trout responses to Y. ruckeri infection, and signi cant changes in the expression of splenic immune-related genes have been detected following Y. ruckeri challenge [5,6]. However, no systematic analyses of patterns of rainbow trout splenic gene expression after Y. ruckeri infection have been conducted to date.…”