Immune Fluorescence Technique and the Isoantigenicity of Mammalian Spermatozoa

Beck, John S.; Edwards, R. G.; Young, Matthew R.

doi:10.1530/jrf.0.0040103

Cited by 41 publications

(13 citation statements)

References 18 publications

(25 reference statements)

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“…With fixed spermatozoa, a weak reaction between human spermatozoa and -globulin from several species is detectable by immunofluorescence in the acrosome (Beck, Edwards & Young, 1962). The different results of the two experiments might be due to changes in the spermatozoa induced by fixation in alcohol, which evidently modifies the acrosomal structure (Abraham & Bhargava, 1963 …”

Section: Mixed Agglutination Reactionmentioning

confidence: 99%

Blood Group Antigens on Human Spermatozoa

Edwards¹,

Ferguson²,

Coombs³

1964

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Section: Mixed Agglutination Reactionmentioning

confidence: 99%

Blood Group Antigens on Human Spermatozoa

Edwards¹,

Ferguson²,

Coombs³

1964

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“…He found that washed ejaculated spermatozoa, which had been killed by alcohol, were all coated, but that about 30% of the spermatozoa in the uterus and oviducts were not coated 7 to 12 hr after coitus ; about the same proportion were motile. Swanson Beck, Edwards & Young (1962) had already raised questions relating to isoantigenicity of spermatozoa and neither they nor Bratanov (1969) or Bedford (1970) found a satisfactory explanation. 302 J.…”

Section: Introductionmentioning

confidence: 99%

Antibodies and Sperm Survival in the Female Tract of the Mouse and Rabbit

Cohen¹,

Werrett²

1975

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Rabbit and mouse spermatozoa from male and female tracts have been examined for their species-antigenic surface character, and for adherent antibodies, by double immunofluorescence techniques. Mouse spermatozoa from the ductus deferens showed an area over the acrosome which was positive to anti-mouse serum that had been absorbed with some male mouse somatic tissues including blood, but those from the uterus and oviduct were not stained. Spermatozoa from the uterus were shown to have an antibody coat on the acrosome, with anti-mouse IgG, but those from the ductus deferens and oviduct did not. Rabbit spermatozoa were more variable but their activity was similar: ejaculated spermatozoa sometimes already had antibody of male origin; the majority of the spermatozoa arriving early in the uterus were coated, but in general those that attained the oviducts were not coated. The results are interpreted as evidence for selection by the female tract of a small antigenically different population; the majority of spermatozoa are rejected and/or destroyed.

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Reaction of Spermatozoa With Uterine and Serum Globulin Determined by Immunofluorescence

Symons¹

1967

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A naturally-occurring, heat-stable serum factor reacting with homologous and heterologous spermatozoa has been demonstrated by immunofluorescence (Beck, Edwards & Young, 1962). The widespread incidence of the serum factor in both sexes of several species suggests that it may have some functional significance in sperm physiology. Spermatozoa do not normally contact blood serum but are suspended in fluids of the genital tract, which may contain protein of serum origin, for it has recently been shown that modified immune globulins pass into the rabbit genital tract (Kirton, Desjardins & Hafs, 1966). The oestrous uterus exerts a modifying effect upon spermatozoa; the change, termed capacitation, being a necessary preparation of spermatozoa for fertilization (Austin, 1951;Chang, 1951). In the rabbit capacitation may involve a surface change of the sperm head (Bedford, 1965), although the character of the process is not yet clear. Immunofluorescence detects surface phenomena and has been used in the present study to examine the combination of rabbit globulin with uterine and Fallopian tube spermatozoa. Ejaculated rabbit spermatozoa and epididymal mouse and guinea-pig spermatozoa were also studied in vitro.Oestrous rabbits of mixed breed were mated two or more times with two bucks of proven fertility. Seven to 12 hours p.c., the uterine and tubal contents were washed out separately with Ringer\p=m-\Locke solution at 37\s=deg\C and then recovered by centrifugation before resuspension in a small volume of Ringer\p=m-\ Locke. Suspensions were incubated with anti-rabbit globulin conjugated with fluorescein isothiocyanate (Difco) for 30 min at room temperature. After washing with two changes of Ringer's solution, spermatozoa were mounted in buffered glycerol, pH 7-0, for fluorescence microscopy (Reichert Fluorex; UG1/1-5 primary Alter, Wratten 2B secondary filter, dark ground illumina¬ tion) .Living ejaculated rabbit spermatozoa and epididymal mouse spermatozoa were incubated with heat-inactivated normal rabbit serum diluted with an equal volume of phosphate-buffered saline, pH 7-0, for 30 min. Guinea-pig epididymal spermatozoa were similarly incubated with fresh and heat-in¬ activated homologous serum. After washing, spermatozoa were treated with fluorescein conjugate and examined as above. Control preparations of ejaculated 163

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Immune Fluorescence Technique and the Isoantigenicity of Mammalian Spermatozoa

Cited by 41 publications

References 18 publications

Blood Group Antigens on Human Spermatozoa

Blood Group Antigens on Human Spermatozoa

Antibodies and Sperm Survival in the Female Tract of the Mouse and Rabbit

Reaction of Spermatozoa With Uterine and Serum Globulin Determined by Immunofluorescence

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