Immune factors and health of Antarctic explorers

Kozeretska, І.; Deineko, I.; Anoshko, Yaroslava I.; Shapovalenko, N.; Stamboli, L.V.; Dons’koi, Boris V.

doi:10.33275/1727-7485.2.2021.680

Cited by 6 publications

(3 citation statements)

References 30 publications

(27 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Misbalance results can lead to unfavorable conditions for pregnancy outcomes. Previously, we have shown that a similar misbalance in NK populations was also associated with an unfavorable health status during an Antarctic expedition [34]. Therefore, it is necessary to conduct a deeper and more detailed study of the physiological and phenotypic properties of different NKp46 populations.…”

Section: Discussionmentioning

confidence: 99%

A Blinded Investigation: Accentuated NK Lymphocyte CD335 (NKp46) Expression Predicts Pregnancy Failures

et al. 2023

View full text Add to dashboard Cite

Aim: NKp46 is an NK cell receptor uniquely expressed by NK cells and a small subset of innate lymphoid cells. In our previous studies, we suggested a tight connection between the activity of NK cells and the expression of NKp46 and supported the clinical significance of NKp46 expression in NK cells in women with reproductive failures. In this study, we investigated the expression of NKp46 in NK cells in the peripheral blood of women in early pregnancy and analyzed its association with pregnancy loss. Methods: In a blinded study, we examined blood samples and analyzed the subsequent pregnancy outcomes from 98 early pregnant women (5th–7th week of gestation—w.g.) and 66 women in the 11th–13th week of pregnancy who served as controls. We studied the expression of NKp46 and the levels of anti-cardiolipin antibodies (aCL). The results of aCL were shared with the clinic, while the expression of NKp46 was blinded and not analyzed until the end of the study. Results: A misbalance in the NKp46+NK cells subpopulations was associated with an unfavorable ongoing pregnancy. A decreased level of NKp46high cells (<14%) was strongly associated with miscarriage. A decreased level of the double-bright subpopulation (NKp46hightCD56++) also was a negative prognostic factor for the pregnancy course, but its increased level (>4%) was strongly associated with a successful pregnancy course. Conclusions: Our results showed that accentuated levels of NKp46+NK cells lead to a negative prognosis for early pregnancy courses in women.

show abstract

Section: Discussionmentioning

confidence: 99%

A Blinded Investigation: Accentuated NK Lymphocyte CD335 (NKp46) Expression Predicts Pregnancy Failures

et al. 2023

View full text Add to dashboard Cite

show abstract

“…Flow cytometry was used for imunophenotype study (as shown in Zabara et al 2021). Peripheral blood was incubated with monoclonal antibodies labeled with FITC, PE, and PeCy5 according to the manufacturer's instructions (BD Biosciences, USA).…”

Section: Immunophenotyping Of Lymphocytes By Flow Cytometrymentioning

confidence: 99%

Immune accentuations as a prognostic factor for adaptation in Antarctic conditions

Dubrovskyi,

Zabara,

Anoshko

et al. 2024

Preprint

View full text Add to dashboard Cite

Human presence and work in Antarctica occur under challenging climatic, psychophysiological, and social conditions. The health of expedition participants is a critical factor, and a pre-expedition medical assessment is extremely important. The immune system plays a crucial role in maintaining satisfactory health and in the successful adaptation to adverse living conditions. In the current study, we evaluated the prognostic significance of previously identified accentuations on the health status of participants and their adaptation to Antarctic conditions. Immunophenotyping was performed on 43 male Ukrainian antarctic expedition (UAE) members before the expedition and after their return, assessing the dynamics of laboratory indicators and conducting a detailed analysis of their health status throughout the entire expedition. According to the results, UAE participants with favorable and unfavorable adaptation showed differences in immunophenotypic indicators. The majority of UAE participants with favorable adaptation had T-helper (CD3 + CD4+) levels (> 35%), balanced (< 24%) expression of HLA-DR on cytotoxic T-lymphocytes (CD4 + CD8+), and proportions and absolute levels of NK-lymphocytes (5–18% and 100–400). In contrast, UAE participants with unfavorable adaptation exhibited balanced levels significantly less frequently. NK cell levels within the reference (favorable) range (5–18%) were virtually absent in UAE participants with unfavorable adaptation (1/12), whereas they were typical in participants with favorable adaptation. The combination of two diagnostically significant immune parameters yields higher accuracy. Increased expression of HLA-DR on cytotoxic T-lymphocytes and elevated NK-lymphocyte content significantly enhance the prognostic value of the diagnostic algorithm. Thus, in the group of participants with favorable adaptation, only 9 (30%) had one of these accentuations, while in the group with unfavorable adaptation, all 12 participants had one of these accentuations (P < 0.0001, OR = 59.211). In all Antarctic expedition participants, cortisol levels significantly increased in the first quarter compared to baseline values, followed by dynamic fluctuations throughout the expedition. Cortisol levels returned to baseline after the expedition. The results demonstrate that staying in Antarctica requires adaptive processes that are directly dependent on the balanced function of the immune system. Chronic stress, in conjunction with immune accentuations, complicates successful adaptation. Further investigation into immune processes and adaptation characteristics will allow for the prediction of the risk of adverse adaptation processes, improve participant selection, and facilitate the implementation of proactive measures to prevent the deterioration of the health status of polar expedition members.

show abstract

“…High levels of immune cell activation HLA-DR expression on T and NK cells is a factor of an unfavorable prognosis in reproduction [13,14] and similar levels are a factor of an unfavorable state of health during adaptation among participants of the Antarctic expedition [15]. But reduced activation of NK-lymphocytes may contribute to increased susceptibility to viral infections and bacterial complications [16].…”

Section: Introductionmentioning

confidence: 99%

Theory of immune accentuations: formation of accentuated immunophenotype

Dons’koi

2023

Lik. sprava

View full text Add to dashboard Cite

In numerous previous studies, we have formulated the idea of immune accentuation, demonstrated associations between immune accentuations and the unfavorable course of physiological processes, and shown that the association of several accentuations has a reliable negative effect on the further course of the reproductive process. In the article, we demonstrated the correlative and associative nature of certain immune accentuations, which explains the causes of the formation of an unfavorable immuno-accentuated phenotype. Thus, we found highly reliable correlative and associative relationships between the parameters of natural resistance (NK quantity, NK cytotoxicity, expression of CD158a and CD8 on NK cells), all these parameters were mutually correlated with each other and their accentuations were associated with accentuations of partner parameters. Another immuno-accented cluster had pro-inflammatory features, so the expression of HLA-DR on NK and T-cytotoxic cells, as well as NKT-like populations (CD3+CD56+ and CD3+CD158a+) also had highly reliable correlations and associations. This pro-inflammatory phenotype was negatively correlated and inversely associated with T-helper levels. Presumably, this is a consequence of uncontrolled chronic activation with the appearance of alternative ways of differentiation of T cells that bypass regulatory control by T-helpers. We showed a fundamental difference in the association and correlative relations between immune parameters depending on their level. Thus, HLA-DR levels on NK cells within (NK < 24 %) had a negative correlation with % NK cells, while, within (NK > 24 %) they begin to correlate positively and reliably with the number of NK. So, accentuation is the place in the distribution of the parameter, where the parameter begins to put pressure on other parameters and determine their level of direction. These are the same zones where the parameter becomes unfavorable, because it loses the possibility of regulation. Accentuation of the immune parameter is associated with specific accentuations of other parameters. Whether it causes changes in other links or whether it and other changes are the result of extra-immune or environmental factors are unknown. But it is clear that this leads to the formation of an immuno-accented phenotype. Accentuation creates prerequisites for the formation of other immune accentuations due to the imbalance of systemic immune regulation.

show abstract

Immune factors and health of Antarctic explorers

Cited by 6 publications

References 30 publications

A Blinded Investigation: Accentuated NK Lymphocyte CD335 (NKp46) Expression Predicts Pregnancy Failures

A Blinded Investigation: Accentuated NK Lymphocyte CD335 (NKp46) Expression Predicts Pregnancy Failures

Immune accentuations as a prognostic factor for adaptation in Antarctic conditions

Theory of immune accentuations: formation of accentuated immunophenotype

Contact Info

Product

Resources

About