Immune electron microscopy of cross-reactions between Mycoplasma pneumoniae and human erythrocytes

Deas, Jane E.; Janney, F A; Lee, L T; Howe, Calderón

doi:10.1128/iai.24.1.211-217.1979

Cited by 14 publications

(4 citation statements)

References 12 publications

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“…Consistently, in vitro experiments have showed that MP can adhere to human red blood cells. 10,11 Recently, Deas et al 12 discovered interactions between MP and human blood red cells via scanning and transmission electron microscopy experiments. Hence, a potential mechanism was proposed: MP adheres to red blood cells and then disseminates from the lungs to other sites.…”

Section: Discussionmentioning

confidence: 99%

Prevalence and clinical characteristics of septicemia in children with Mycoplasma pneumoniae pneumonia

et al. 2021

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Background Mycoplasma pneumoniae (MP) pneumonia in children can be challenging to treat, and the impact of MP blood infection is unclear. The present study aims to determine the prevalence and clinical characteristics of MP septicemia among pediatric patients. Methods Children hospitalized at our center for MP pneumonia between October 2017 and June 2018 were included. Healthy controls visiting our outpatient clinic for regular physical examinations were also enrolled. MP was detected by real-time polymerase chain reaction (qPCR) analysis of plasma and peripheral blood mononuclear cell (PBMC) samples. Results Sixty-one children with MP pneumonia and 30 healthy children were included. Among children with MP infection, 31 (50.8%) were positive for MP by qPCR (19 in plasma samples, 8 in PBMC samples, and 4 in both). All healthy controls were negative for MP by qPCR. Conclusions The prevalence of MP septicemia in children with MP pneumonia is moderate. However, detection of MP in blood samples may have limited clinical value for guiding treatment.

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Section: Discussionmentioning

confidence: 99%

Prevalence and clinical characteristics of septicemia in children with Mycoplasma pneumoniae pneumonia

et al. 2021

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“…Cold agglutinins are a common phenomenon in human patients with mycoplasma pneumonias, occurring in 50–75% of cases . These antibodies are directed against mycoplasma membrane proteins and cross‐react with the human RBC surface glycoprotein antigen I . This epitope, comprised of N ‐acetyl‐lactosamine subunits, is included in human blood typing, and is also expressed on canine erythrocytes …”

Section: Discussionmentioning

confidence: 99%

“…Mycoplasma‐associated cold agglutinins are typically, but not always, polyclonal IgM antibodies . Agglutination occurs between 4 and 20°C, but not at normal core body temperatures.…”

Section: Discussionmentioning

confidence: 99%

Transient cold agglutinins associated withMycoplasma cynospneumonia in a dog

Pinkos

Friedrichs

Monaghan

et al. 2015

Veterinary Clinical Pathol

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This report details a case of reversible cold agglutinins in a dog with Mycoplasma cynos pneumonia. An 11-month-old female spayed Rhodesian Ridgeback was presented for lethargy and cough. Thoracic radiographs revealed an alveolar pattern present bilaterally in the cranioventral lung lobes. Septic neutrophilic inflammation with suspected Mycoplasma sp. organisms was noted on cytologic examination of a trans-tracheal wash, and the dog was treated empirically with IV ampicillin/sulbactam and enrofloxacin pending culture results. Red blood cell agglutination was noted unexpectedly on several blood film reviews during hospitalization; however, the dog never developed clinical or laboratory evidence of hemolysis. Cold agglutinins were demonstrated based on the results of a saline dilution and cold agglutinin test that showed agglutination at 4°C but not at room temperature (21°C) or 37°C. Based on a positive culture for M cynos, the dog was treated for 8 weeks with oral enrofloxacin. After clinical and radiographic resolution of the pneumonia, repeated saline dilution and cold agglutinin tests of peripheral blood were negative at all temperatures. Reversible, asymptomatic cold agglutinins are common in human patients with mycoplasma pneumonia, but this is the first reported case in a dog.

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“…Furthermore, it appeared that only filamentous organisms in which the tip was in close association with the ciliary epithelium caused host cell injury (8). However, whereas it is evident from these studies that M. pneumoniae attach by means of their tip structures to ciliated epithelial cells, it has also been shown that this is not necessarily the case when they attach to other mammalian cells (6,10). Kahane et al (13) have reviewed the evidence for the attachment site of mycoplasmas to erythrocytes.…”

Section: L 4pmentioning

confidence: 99%

Freeze-fracture confirmation of the presence of a core in the specialized tip structure of Mycoplasma pneumoniae

Wall¹,

Pfister²,

Somerson³

1983

J Bacteriol

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The presence of a specialized terminal region in Mycoplasma pneumoniae was seen in thin sections viewed in an electron microscope. Actively growing cells were examined by the freeze-fracture technique in the absence of fixation to further establish the core as a significant structural entity. Cross fractures revealed a cytoplasmic matrix surrounding a central core structure of about 54 nm. This structure disappeared rapidly in aging cells. The convex protoplastic faces of the membrane around the core had characteristic 5-to 10-nm intramembrane particles evenly distributed across the cell surface, with no apparent difference in the region of the specialized tip. A periodicity previously noted in negatively stained preparations was clearly defined here in thin sections. Attachment of actively growing cells to sheep erythrocytes was seen primarily as a side attachment rather than attachment at the tip alone. This association between the mycoplasma and the sheep erythrocytes seriously deformed the sheep erythrocytes, but no membrane fusion could be detected.

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Immune electron microscopy of cross-reactions between Mycoplasma pneumoniae and human erythrocytes

Cited by 14 publications

References 12 publications

Prevalence and clinical characteristics of septicemia in children with Mycoplasma pneumoniae pneumonia

Prevalence and clinical characteristics of septicemia in children with Mycoplasma pneumoniae pneumonia

Transient cold agglutinins associated withMycoplasma cynospneumonia in a dog

Freeze-fracture confirmation of the presence of a core in the specialized tip structure of Mycoplasma pneumoniae

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