Immune efficacy of five novel recombinant Bordetella bronchiseptica proteins

Abstract

BackgroundThe Gram-negative pathogen Bordetella bronchiseptica causes acute and chronic respiratory infection in a variety of animals. Currently, there is no vaccine to prevent these infections. To identify useful candidate antigens for such a vaccine, five B. bronchiseptica genes including amino acid ATP-binding cassette transporter substrate-binding protein (ABC), lipoprotein (PL), outer membrane porin protein (PPP), leu/ile/val-binding protein (BPP), and conserved hypothetical protein (CHP) were cloned and the recombinant proteins were expressed. The immune responses of mice to vaccination with individual recombinant proteins were measured.ResultsEach of the tested recombinant proteins induced a high antibody titer. PPP and PL showed protective indices against challenges with B. bronchiseptica. The protection ratios were 62.5 and 50 %, respectively, compared with 12.5 % for control vaccinations. The protection ratios of ABC, BPP, and CHP were not significantly different from the controls. IgG-subtype and cytokine analysis demonstrated that PPP and PL can induce two immune responses: a humoral immune response and a cell-mediated immune response. The humoral immunity-mediated, Th2-type response dominated.ConclusionThe identification of PPP and PL, which offer immune-protective potential, identifies them as candidates for the development of a diagnostic test or a vaccine for B. bronchiseptica.

Immunogenicity of recombinant outer membrane porin protein and protective efficacy against lethal challenge with Bordetella bronchiseptica in rabbits

“…Previously, challenge protection studies in mice revealed five types of immune proteins from B. bronchiseptica that served as candidate antigens for the rabbit subunit vaccine, and the highest protection rate was observed for the outer membrane porin (Liu et al . ). In the present study, the OMPP of B. bronchiseptica was successfully expressed in E. coli .…”

“…The protective effects of the outer membrane porin protein (OMPP) and putative lipoprotein were better than the other proteins, and the protection rates were 62·5 and 50% respectively (Liu et al . ). Therefore, the purpose of this study was to determine whether OMPP represents a potentially useful vaccine immunogen for rabbits against B. bronchiseptica .…”

“…Then, five of these newly discovered immunogenic proteins were selected for the expression of the recombinant proteins in prokaryotic systems, and the immunogenicity of each recombinant protein and their abilities to protect mice from B. bronchiseptica infection were analysed. The protective effects of the outer membrane porin protein (OMPP) and putative lipoprotein were better than the other proteins, and the protection rates were 62Á5 and 50% respectively (Liu et al 2015). Therefore, the purpose of this study was to determine whether OMPP represents a potentially useful vaccine immunogen for rabbits against B. bronchiseptica.…”

Immunogenicity Prediction by VaxiJen: A Ten Year Overview

“…This confirmed the antigenicity of seven of the nine proteins. Bordetella bronchiseptica (B. bronchiseptica) causes acute and chronic respiratory infection in a variety of animals [167]. Currently, there is no vaccine to prevent these infections.…”

“…Currently, there is no vaccine to prevent these infections. Liu et al [167] analyzed five B. bronchiseptica antigens, as defined by VaxiJen, including amino acid ATP-binding cassette transporter substrate-binding protein (ABC), lipoprotein (PL), outer membrane porin protein (PPP), leu/ile/ val-binding protein (BPP), and conserved hypothetical protein (CHP). The murine immune responses to individual recombinant proteins were measured, with each tested protein inducing high antibody titers.…”