Immune complex mediated inflammation in the mouse peritoneal cavity

Blackham, A.; Woods, F.

doi:10.1016/0160-5402(86)90007-0

Cited by 9 publications

(4 citation statements)

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“…Xylene-induced auricle swelling and acetic acid-induced capillary permeability increase were two classical mouse models widely used in search for drugs with anti-inflammatory activity (Blackham and Woods, 1986; Weirich et al, 1977). Consistent to previous reports (Chen et al, 2002, 2003; Tanaka et al, 1986; Wang et al, 1995), shikonin efficiently inhibited auricle swelling (Fig.…”

Section: Resultsmentioning

confidence: 99%

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Shikonin extracted from medicinal Chinese herbs exerts anti-inflammatory effect via proteasome inhibition

Lu¹,

Qin²,

Huang³

et al. 2011

European Journal of Pharmacology

138

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Shikonin, extracted from medicinal Chinese herb (Lithospermum erythrorhizo), was reported to exert anti-inflammatory and anti-cancer effects both in vitro and in vivo. We have found that proteasome was a molecular target of shikonin in tumor cells, but whether shikonin targets macrophage proteasome needs to be investigated. In the current study, we report that shikonin inhibited inflammation in mouse models as efficiently as dexamethasone. Shikonin at 4 μM reduced the Lipopolysaccharides (LPS)-mediated TNFα release in rat primary macrophage cultures, and blocked the translocation of p65-NF-κB from the cytoplasm to the nucleus, associated with decreased proteasomal activity. Consistently, shikonin accumulated IκB-α, an inhibitor of NF-κB, and ubiquitinated proteins in rat primary macrophage cultures, demonstrating that the proteasome is a target of shikonin under inflammatory conditions. Shikonin also induced macrophage cell apoptosis and cell death. These results demonstrate for the first time that proteasome inhibition by shikonin contributes to its anti-inflammatory effect. The novel finding about macrophage proteasome as a target of shikonin suggests that this medicinal compound has great potential to be developed into an anti-inflammatory agent.

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Section: Resultsmentioning

confidence: 99%

“…The model of increased capillary permeability in mice was induced by acetic acid (Blackham and Woods, 1986). Total 60 NIH mice were randomly divided into 6 groups: DMSO; LPS (35 mg/kg); LPS+shi (0.5 mg/kg); LPS+shi (1 mg/kg); LPS+shi (4 mg/kg) and LPS dexamethasone (2.5 mg/kg).…”

Section: Methodsmentioning

confidence: 99%

Shikonin extracted from medicinal Chinese herbs exerts anti-inflammatory effect via proteasome inhibition

Lu¹,

Qin²,

Huang³

et al. 2011

European Journal of Pharmacology

138

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“…Reversibility was demonstrated by incubation of compound, 1-10 gM, with enzyme for 4 min at room temperature followed by removal of the compound by centrifugation through a 1 ml G50 sephadex column after the method of Penefsky [11]. Samples were taken for assay pre-and postcolumn and activity compared with controls incubated without compound.…”

Section: Reversibility Of Compound Activitymentioning

confidence: 99%

“…Peritoneal inflammation was induced in female LACA mice (weight range 20-30 g) according to the method of Blackham & Woods, [11]. Briefly each mouse received 0.25 ml of 0.5% pontamine sky blue in saline intravenously immediately before an intraperitoneal injection of 0.75 ml of washed immune complex (200 lag ovalbumin & equivalence of rabbit antiserum).…”

Section: Immune Complex-induced Peritoneal Inflammationmentioning

confidence: 99%

FPL 62064, a topically active 5-lipoxygenase/cyclooxygenase inhibitor

Blackham¹,

Griffiths²,

Hallam³

et al. 1990

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FLP 62064 [N-(4-methoxyphenyl)-1-phenyl-1H-pyrazole-3-amine] is a dual inhibitor of prostaglandin synthetase and 5-lipoxygenase. The compound had anti-inflammatory activity in vivo in a number of models. It inhibited peritoneal inflammation induced by immune-complex when given locally. When applied to the skin, FPL 62064 inhibited UV irradiation-induced erythema and PGE2 formation in the guinea pig and also oedema formation and eicosanoid production in the mouse ear produced by arachidonic acid. Co-injected with arachidonic acid in rabbit skin, FPL 62064 inhibited oedema and eicosanoid formation.

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