Immortalized and tumorigenic adult human prostatic epithelial cell lines: Characteristics and applications part I. Cell markers and immortalized nontumorigenic cell lines

Webber, Mukta M.; Bello, Diana; Quader, Salmaan T.A.

doi:10.1002/(sici)1097-0045(199612)29:6<386::aid-pros7>3.0.co;2-6

Cited by 78 publications

(40 citation statements)

References 36 publications

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“…In the XTT assay analysis in this study, the LNCaP cell line was most sensitive compared to PC3 and TSU-Pr1 cells. This could possibly be explained by the fact that LNCaP cells are known to be a cell line expressing prostatic markers and being hormone sensitive, whereas PC3 and TSU-Pr1 cells are less differentiated (Kaighn et al, 1979;Horoszewicz et al, 1983;Webber et al, 1996). PC3 and TSU-Pr1 cells carry molecular alterations such as a mutated p53 and the origin of TSU-Pr1 cells is discussed controversially (Iizumi et al, 1987;van-Bokhoven et al, 2001).…”

Section: Discussionmentioning

confidence: 99%

Sodium butyrate and tributyrin induce in vivo growth inhibition and apoptosis in human prostate cancer

et al. 2004

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Histone deacetylase inhibitors (HDACs) are known to exhibit antiproliferative effects on various carcinoma cells. In this study, the in vivo efficiency of two HDACs, sodium butyrate and tributyrin, on prostate cancer growth inhibition were investigated. To gain an insight into the possible underlying pathways, cell culture experiments were performed focusing on the expression of p21, Rb and cmyc. For in vivo testing, prostate cancer cell lines (PC3 and TSU-Pr1) were seeded on the chorioallantois membrane (CAM) and implanted in a xenograft model using nude mice. Standard Western blot analysis was performed for protein expression of p21, Rb and c-myc in HDAC-treated vs untreated prostate cancer cells. Both sodium butyrate and tributyrin had a considerable treatment effect on microtumours on the chicken egg at already very low concentrations of 0.1 mM. Tributyrin-treated tumours showed the strongest effect with 38% apoptotic nuclei in the prostate cancer cell line PC3. In the mouse model, there was almost no difference between sodium butyrate and tributyrin. In untreated animals the tumours were almost double the size 4 weeks after implantation. Tumours of the treatment groups had a significantly lower percentage of Ki-67-positive-stained nuclei. As demonstrated by Western blot analysis, these effects seem to be independent of p53 status and a pathway via p21 -Rb -c-myc is possibly involved. In this study we have demonstrated a substantial in vivo treatment effect, which can be induced by the application of sodium butyrate or the orally applicable tributyrin in human prostate cancer. The given results may provide the rationale to apply these drugs in well-controlled clinical trials in patients being at high risk of recurrence after specific therapy or in patients with locally or distant advanced prostate cancer.

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Section: Discussionmentioning

confidence: 99%

Sodium butyrate and tributyrin induce in vivo growth inhibition and apoptosis in human prostate cancer

et al. 2004

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“…Although generation of primary cultures of mouse prostatic cells is becoming more frequent, this article also will not include any information about rodent cell culture models. Finally, although cell lines created by introduction of immortalizing genes into primary cultures are often used as representative of the normal or cancer cells of origin (for reviews see Webber et al 1996Webber et al , 1997, studies with these cells also will not be included here because the relevance of these lines to normal or cancer biology is controversial.…”

Section: Introductionmentioning

confidence: 99%

Primary cell cultures as models of prostate cancer development

Peehl¹

2005

Endocr Relat Cancer

152

142

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This review focuses on primary cultures of human prostatic epithelial cells and their applications as models of normal and malignant biological behavior. Current abilities to culture cells from normal tissues, from premalignant dysplastic lesions (prostatic intraepithelial neoplasia), from primary adenocarcinomas, and from metastases are described. Evidence for representation of the interrelated cells of the normal prostatic epithelium -stem cells, basal epithelial cells, secretory epithelial cells, transit amplifying cells and neuroendocrine cells -in primary cultures is presented. Comparisons between normal and cancer-derived primary cultures are made regarding biological activities relevant to carcinogenesis, such as proliferation, apoptosis, differentiation, senescence, adhesion, migration, invasion, steroid hormone metabolism, other metabolic pathways and angiogenesis. Analyses of tumor suppressor activity, differential gene expression and cytogenetics in primary cultures have revealed changes relevant to prostate cancer progression. Preclinical studies with primary cultures have provided information useful for designing new strategies for chemoprevention, chemotherapy, cytotoxin therapy, radiation therapy, gene therapy and imaging. While the behavior of normal primary cultures is often used as a basis for comparison with established, immortal prostate cancer cell lines, the most informative studies are performed with donor-matched pairs of normal and malignant primary cultures, grown under identical conditions. Challenges that remain to be addressed if the full potential of primary cultures as a model system is to be realized include isolation, culture and characterization of stem cells, improved methodology to induce or maintain a fully differentiated, androgen-responsive phenotype, and identification of cell surface antigens or other markers with which to purify pure populations of live cancer or premalignant cells apart from non-malignant epithelial cells prior to culture.

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“…Evaluation of androgen responsiveness is complex, can change with a change in growth conditions and is beyond the scope of the present study. 27 Having con®rmed the validity of the model system, studies then sought to evaluate potential differential affects upon normal or early cell lines, as compared to those representative of advanced disease. Adriamycin and vinblastine both possess signi®cant single agent activity in a wide array of neoplasms, including prostate and thus represent non-selective globally active agents.…”

Section: Discussionmentioning

confidence: 99%

“…27 RNA was isolated from each cell line and subjected to cDNA synthesis and PCR ampli®cation for either PSA or AR. In each instance, beta-actin was also ampli®ed to verify RNA integrity and cDNA synthesis.…”

Section: Detection Of Prostate Speci®c Antigen (Psa) and Androgen Recmentioning

confidence: 99%

Prostate cancer chemoprevention agents exhibit selective activity against early stage prostate cancer cells

Kyle

Patel

et al. 2001

Prostate Cancer Prostatic Dis

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Preclinical models for the identi®cation of prostate cancer chemoprevention agents are lacking. Based upon the notion that clinically useful chemoprevention agents should exhibit selective activity against early stage disease, studies were undertaken to assess whether chemoprevention agents selectively inhibited the growth of early stage prostate cancer, as compared to late stage cancer. First, a series of cell and molecular studies were performed, which, when taken together, validated the use of a panel of prostate cell lines as a model of the different stages of carcinogenesis. Next, therapeutic responsiveness to ten different cytotoxic or chemoprevention agents was evaluated. Chemoprevention agents exhibited selective activity against normal and early transformed prostate tissue, whereas cytotoxic agents were non-speci®c. Selective activity against early versus advanced prostate cancer cells is identi®ed as a potential screening method for chemoprevention agents. Prostate Cancer and Prostatic Diseases (2001) 4, 81±91.

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Immortalized and tumorigenic adult human prostatic epithelial cell lines: Characteristics and applications part I. Cell markers and immortalized nontumorigenic cell lines

Cited by 78 publications

References 36 publications

Sodium butyrate and tributyrin induce in vivo growth inhibition and apoptosis in human prostate cancer

Sodium butyrate and tributyrin induce in vivo growth inhibition and apoptosis in human prostate cancer

Primary cell cultures as models of prostate cancer development

Prostate cancer chemoprevention agents exhibit selective activity against early stage prostate cancer cells

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