Immortalization of porcine placental trophoblast cells through reconstitution of telomerase activity

Zhang, Hongling; Huang, Yong; Wang, Lili; Yu, Tingting; Wang, Zengguo; Chang, Lingling; Zhao, Xiaomin; Luo, Xiaomao; Zhang, Liang; Tong, Dewen

doi:10.1016/j.theriogenology.2016.01.006

Cited by 18 publications

(20 citation statements)

References 36 publications

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“…Our finding is in agreement with the previous report demonstrating the role of lncRNAs in regulating the gene expression in the placentas of pigs [44]. On the other hand, in addition to vasculature development, it has been documented that the placental trophoblast cells undergo differentiation and change in cell shape during the development of the placental fold in pigs [3,[45][46][47][48]. Our DElncRNA-DEgene co-expression network analysis revealed that these DElncRNAs were mainly enriched in pathways of cell adhesion, cytoskeleton organization, epithelial cell differentiation and angiogenesis, indicating that the DElncRNAs are related to the major events that occur during the placental fold development [4,11,45,49,50].…”

Section: Discussionsupporting

confidence: 93%

Transcriptomic and ChIP-seq Integrative Analysis Reveals Important Roles of Epigenetically Regulated lncRNAs in Placental Development in Meishan Pigs

Deng

Tan

Han

et al. 2020

Genes

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The development of the placental fold, which increases the maternal–fetal interacting surface area, is of primary importance for the growth of the fetus throughout the whole pregnancy. However, the mechanisms involved remain to be fully elucidated. Increasing evidence has revealed that long non-coding RNAs (lncRNAs) are a new class of RNAs with regulatory functions and could be epigenetically regulated by histone modifications. In this study, 141 lncRNAs (including 73 up-regulated and 68 down-regulated lncRNAs) were identified to be differentially expressed in the placentas of pigs during the establishment and expanding stages of placental fold development. The differentially expressed lncRNAs and genes (DElncRNA-DEgene) co-expression network analysis revealed that these differentially expressed lncRNAs (DElncRNAs) were mainly enriched in pathways of cell adhesion, cytoskeleton organization, epithelial cell differentiation and angiogenesis, indicating that the DElncRNAs are related to the major events that occur during placental fold development. In addition, we integrated the RNA-seq (RNA sequencing) data with the ChIP-seq (chromatin immunoprecipitation sequencing) data of H3K4me3/H3K27ac produced from the placental samples of pigs from the two stages (gestational days 50 and 95). The analysis revealed that the changes in H3K4me3 and/or H3K27ac levels were significantly associated with the changes in the expression levels of 37 DElncRNAs. Furthermore, several H3K4me3/H3K27ac-lncRNAs were characterized to be significantly correlated with genes functionally related to placental development. Thus, this study provides new insights into understanding the mechanisms for the placental development of pigs.

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Section: Discussionsupporting

confidence: 93%

Transcriptomic and ChIP-seq Integrative Analysis Reveals Important Roles of Epigenetically Regulated lncRNAs in Placental Development in Meishan Pigs

Deng

Tan

Han

et al. 2020

Genes

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“…Pellets were then resuspended in DMEM/F12 and deposited on top of a discontinuous 35% and 45% (V/V) Percoll (Pharmacia, London, UK) gradient solution, and centrifuged at 2000× g for 20 min. Villous cytotrophoblasts were collected from the appropriate layers, and cultured in DMEM/F12 supplemented with 10% FBS (HyClone, Logan, UT, USA), 1% (V/V) Insulin-Transferrin-Selenium (ITS; Sigma, Saint Louis, MO, USA), 10 ng/mL of epidermal growth factor (EGF; Invitrogen, Carlsbad, CA, USA), 100 U/mL penicillin, and 100 µg/mL streptomycin at 37 • C under 5% CO 2 as previously reported [19,20]. The purity of preparations of cytotrophoblasts isolated from full-term placentas was evaluated by flow cytometry as previously described [20], using FITC fluorescein-labeled antibody against cytokeratin-7 (Santa Cruz Tech, Dallas, CA, USA) as a specific marker of trophoblast cells.…”

Section: Cell Culture and Drug Treatmentmentioning

confidence: 99%

Impaired Mitochondrial Function Results from Oxidative Stress in the Full-Term Placenta of Sows with Excessive Back-Fat

Liang

Huang

Wen

et al. 2020

Animals

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The aim of this study was to determine the effect of excessive back-fat (BF) of sows on placental oxidative stress, ATP generation, mitochondrial alterations in content and structure, and mitochondrial function in isolated trophoblasts. Placental tissue was collected by vaginal delivery from BFI (15–20 mm, n = 10) and BFII (21–27 mm, n = 10) sows formed according to BF at mating. Our results demonstrated that excessive back-fat contributed to augmented oxidative stress in term placenta, as evidenced by excessive production of ROS, elevated protein carbonylation, and reduced SOD, GSH-PX, and CAT activities (p < 0.05). Indicative of mitochondrial dysfunction, reduced mitochondrial respiration in cultured trophoblasts was linked to decreased ATP generation, lower mitochondrial Complex I activity and reduced expression of electron transport chain subunits in placenta of BFII sows (p < 0.05). Meanwhile, we observed negative alterations in mitochondrial biogenesis and structure in the placenta from BFII group (p < 0.05). Finally, our in vitro studies showed lipid-induced ROS production resulted in mitochondrial alterations in trophoblasts, and these effects were blocked by antioxidant treatment. Together, these data reveal that excessive back-fat aggravates mitochondrial injury induced by increased oxidative stress in pig term placenta, which may have detrimental consequences on placental function and therefore impaired fetal growth and development.

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“…T cell lines (as well as E cell lines), easy to obtain from early blastocysts, have characteristics of T stem cells, according to Hou et al (2015). Finally T cell lines may be immortalized (Zhang et al, 2016). The T and E cell lines constitute models of extraembryonic epithelial cells useful for in vitro experiments.…”

Section: Resultsmentioning

confidence: 99%

Planar polarity of the extraembryonic epithelia in the preimplantation porcine conceptus

Fléchon¹

2017

Int. J. Dev. Biol.

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The present review deals with the trophoblast structure during the free intrauterine life of the pig blastocyst. The term trophoblast is used here to describe the association of the first extraembryonic cell layers, the trophectoderm and the primitive endoderm that are polarized epithelia, a fact established by ultrastructural and immunocytochemical data. The aim of this synthesis is to gather the relative works dispersed in the litterature and to explain the implication of the planar polarity of these cell layers on their developmental fate and roles. These epithelia are intricately dependent on each other for the maintenance of their differentiated state and continuity. The modalities of their spectacular expansion can be explained in part by biomechanical concepts.

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Immortalization of porcine placental trophoblast cells through reconstitution of telomerase activity

Cited by 18 publications

References 36 publications

Transcriptomic and ChIP-seq Integrative Analysis Reveals Important Roles of Epigenetically Regulated lncRNAs in Placental Development in Meishan Pigs

Transcriptomic and ChIP-seq Integrative Analysis Reveals Important Roles of Epigenetically Regulated lncRNAs in Placental Development in Meishan Pigs

Impaired Mitochondrial Function Results from Oxidative Stress in the Full-Term Placenta of Sows with Excessive Back-Fat

Planar polarity of the extraembryonic epithelia in the preimplantation porcine conceptus

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