Immortalization of Human T Lymphocytes After Transfection of Epstein-Barr Virus DNA

Stevenson, Mario; Volsky, Barbara; Hedenskog, Mona; Volsky, David J.

doi:10.1126/science.3016899

Cited by 61 publications

(20 citation statements)

References 26 publications

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“…It is not impossible that EBV infects some T cells in vivo, especially those that may express EBV-R at a high density. Such infected cells may acquire a transformed phenotype, as was the case with the T cells transfected in vitro with EBV DNA (Stevenson et al, 1986). Furthermore, T cell infection in vivo by EBV might also occur when these cells are persistently exposed to this virus, a likely situation in patients with chronic active EBV infection.…”

Section: Discussionmentioning

confidence: 99%

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Epstein--Barr virus receptor expression on human CD8+ (cytotoxic/suppressor) T lymphocytes

Sauvageau

Stocco

Kasparian

et al. 1990

Journal of General Virology

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In 1977 we showed that cells of a human lymphocytic leukaemia-derived T line (Molt-4) have receptors for Epstein-Barr virus (EBV). More recently, EBVpositive human T cell lymphomas have been recognized and human T cell lines containing the EBV genome have been established in vitro. To understand better the interaction of EBV with T cells, we decided to determine first whether human peripheral blood T lymphocytes express receptors for EBV. Using flow cytometry we examined the binding of both lymphocyte-transforming (B95-8) and non-transforming (P3HR-1) strains of EBV to T lymphocyte subpopulations, using a double labelling technique with T cellspecific phycoerythrinated monoclonal antibodies (Leu 2a) and fluoresceinated viral preparation. Our results suggest that, in general, about 50% of the CD8 + (or suppressor/cytotoxic) T cell subpopulation from both EBV-seropositive and -seronegative individuals can bind EBV. EBV receptor expression on these T cells was about l0 and 51 times less than that on Molt-4 and Raji (an EBV receptor-positive B cell line) cells, respectively. The specificity of this binding was demonstrated by the inhibition of attachment of viral preparations preincubated with a monoclonal antibody directed against the viral ligand (gp240/350), and by preincubating these target T cells with unlabelled virus. We were unable to detect EBV-induced antigens in infected T cells, suggesting that, as in Molt-4 cells, virus internalization may not occur in fresh T cells and/or that the virus receptor may not be completely functional. We were also unable to detect C3d (or CR2) receptors on these T cells, or to inhibit virus attachment by treating the targets with an anti-CR2 monoclonal antibody (OKB7), suggesting that the EBV receptor on CD8 + peripheral blood lymphocytes is different from that on B cells.

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Section: Discussionmentioning

confidence: 99%

“…More recently, the EBV genome was found in T cell lymphomas from three patients with chronic EBV infection (Jones et al, 1988). Furthermore, immortalization of human T cells after EBV DNA transfection has also been reported (Stevenson et al, 1986).…”

Section: Introductionmentioning

confidence: 93%

Epstein--Barr virus receptor expression on human CD8+ (cytotoxic/suppressor) T lymphocytes

Sauvageau

Stocco

Kasparian

et al. 1990

Journal of General Virology

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“…EBV was found to bind to 8-18% of normal human thymphocytes, and was capable of infecting the cells in vitro [18]. Immortalized T lymphoblast cell lines were established in vitro by transfecting normal cord blood T cells with EBV DNA [19]. Tumour tissue from patients with CD4…”

Section: Discussionmentioning

confidence: 99%

“…However, target cells expanded as new and more sensitive detection techniques for EBV became available. Thus, it seems that EBV can infect NK cells [13][14][15][16][17] and T cells [18][19][20][21] other than B cells.…”

Section: Introductionmentioning

confidence: 99%

Natural killer (NK) lymphocytosis induced by Epstein–Barr virus (EBV) reactivation in monoclonal gammopathy of undertermined significance (MGUS)

Ishiyama¹,

Koike²,

Watanabe

et al. 1996

Clinical and Experimental Immunology

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SUMMARYWe investigated the relationship between NK lymphocytosis and EBV infection in MGUS. We found that two out of 10 patients showed an increase of NK cells compared with normal controls. In addition, these two patients had far higher levels of CD5 LOW NK cells (activated NK cells) than controls. EBV DNA was detected by polymerase chain reaction in peripheral blood mononuclear cells from the two patients, although the other eight patients with MGUS and all 20 normal controls had no detectable EBV DNA. Furtheremore, EBV DNA was detected in sorted CD5 LOW NK cells. These results suggest that reactivation of EBV might be related to the increase of NK cells, particularly CD5 LOW NK cells in some patients with MGUS.

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“…EBV was also found to infect primary explant cultures of human ectocervix epithelial cells in vitro (Sixbey et al, 1983). Immortalization of human T lymphocytes after transfection of EBV DNA has been recently reported (Stevenson et al, 1986). T cell lymphomas containing EBV DNA have been described in patients with chronic EBV infections (Jones et al, 1988).…”

Section: Introductfonmentioning

confidence: 96%

Epstein-Barr Virus DNA Sequences in Precursor Monocyte-Macrophage Cell Lines Established from the Bone Marrow of Children with Maturation Defects of Haematopoiesis

Revoltella¹,

Vigneti

Fruscalzo

et al. 1989

Journal of General Virology

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SUMMARYEpstein-Barr virus (EBV) DNA sequences were detected in four established monoblast or early monocytic cell lines (CM-S, ROV-S, CV-S and AD-S) obtained from bone marrow of children suffering from maturation defects of haematopoiesis. EBV is present in these cells in a latent state. The viral DNA in these cell lines was analysed by Southern blot hybridization, using a set of cloned EBV DNA fragments from the EBV strain B95-8 as probes. A common spectrum of highly related but distinguishable EBV DNA restriction enzyme sequences was found, suggesting some genomic diversity. Propagation of the cells in long-term culture revealed a gradual decrease of EBV copies per cell in all lines with some minor changes in the restriction pattern of the EBV DNA. These findings demonstrate that human precursor monocyte cells may be susceptible to infection by EBV.

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Immortalization of Human T Lymphocytes After Transfection of Epstein-Barr Virus DNA

Cited by 61 publications

References 26 publications

Epstein--Barr virus receptor expression on human CD8+ (cytotoxic/suppressor) T lymphocytes

Epstein--Barr virus receptor expression on human CD8+ (cytotoxic/suppressor) T lymphocytes

Natural killer (NK) lymphocytosis induced by Epstein–Barr virus (EBV) reactivation in monoclonal gammopathy of undertermined significance (MGUS)

Epstein-Barr Virus DNA Sequences in Precursor Monocyte-Macrophage Cell Lines Established from the Bone Marrow of Children with Maturation Defects of Haematopoiesis

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