Immortalization of early embryonic cell derivatives after the transfer of the early region of simian virus 40 into F9 teratocarcinoma cells

Sequential onset of three 5‐HT receptors during the 5‐hydroxytryptaminergic differentiation of the murine 1C11 cell line

“…Immortalized precursor cells can be obtained from a multipotential embryonal carcinoma (EC) cell line transfected by a recombinant plasmid PK4 containing the early genes of SV40 under the control of the adenovirus ElA promoter (Kellermann & Kelly, 1986). The resulting immortalized cell lines have properties of committed stem cells, able to differentiate further along a restricted lineage (Kellermann et al, 1987;.…”

Cross-talk between 5-Hydroxytryptamine Receptors in a Serotonergic Cell Line

“…The 1C11 clone was derived from F9 multipotential embryonal carcinoma cells through immortalization and differentiation (2,3). The 1C11 clone has the properties of a neuroectodermal progenitor able to differentiate into 5-hydroxytryptaminergic cells (1C11*) by induction by dibutyryl cyclic AMP (Bt2cAMP) and cyclohexane carboxylic acid (CCA).…”

Serotonin uptake, storage, and synthesis in an immortalized committed cell line derived from mouse teratocarcinoma.

“…The method is based upon the transformation of embryonal carcinoma cells by a recombinant plasmid, pK4, that carries the early region of SV40 under the control of the adenovirus 5 ElA promoter. This promoter is active in early embryonic cells (25). The cell line F9K4b2 was obtained after transfection by pK4 of the embryonal carcinoma F9 (26).…”

“…After in vitro differentiation of F9K4b2 was induced with retinoic acid and dibutyryl cyclic AMP (Bt2cAMP), several clones corresponding to immortalized precursors of the neuroectodermal, mesodermal, and endodermal lineages were selected (27) Cell Culture. Cells were grown as described (25 Tumor Formation. Injection of cells into syngeneic mice and processing of the tumors were as described (27).…”