Immortalization and characterization of human dental pulp cells with odontoblastic differentiation

Kitagawa, Masae; Ueda, Hirota; Iizuka, Shinji; Sakamoto, K.; Oka, Hiroko; Kudo, Yasusei; Ogawa, Ikuko; Miyauchi, Mutsumi; Tahara, Hidetoshi; Takata, Takashi

doi:10.1016/j.archoralbio.2007.02.006

Cited by 105 publications

(81 citation statements)

References 21 publications

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“…The DSPP transcript was predominantly expressed in odontoblasts and transiently in preameloblasts along with an involvement with odontoblast differentiation and mineralization, which is used as an indicator of odontoblastic differentiation (2,61). Dental pulp cells cultured in mineralizing medium exhibited odontoblastic features, including increasing DSPP; undifferentiated dental pulp cells also exhibit DSPP expression (62)(63)(64). In our study, expression of DSPP was weak before Day 7, but the amount increased by Day 10, and after Day 14 DSPP was strongly expressed (Fig.…”

Section: Identification Of Mirnas That Regulate Dspp By Qrt-pcrmentioning

confidence: 68%

miRNA expression profiling identifies DSPP regulators in cultured dental pulp cells

Huang

Xu²,

Gao³

et al. 2011

Int J Mol Med

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Abstract. Dentin sialophosphoprotein (DSPP), an important marker of odontoblast differentiation, is a prerequisite for tooth development and mineralization; however, the molecular mechanisms of both temporal and spatial regulation remain unknown. MicroRNAs (miRNAs) provide an additional level of control beyond that of transcription factors, which regulate post-transcriptional control of gene expression. The present study was designed to provide a first attempt at an in-depth analysis of dental pulp cells at various odontoblastic differentiation stages to obtain miRNA differential expression patterns, and to determine the contribution of miRNAs in the expression of DSPP during odontoblast differentiation. Dual luciferase reporter assays and qRT-PCR were used to validate miRNAs identified from bioinformatic analyses to determine whether they were able to regulate the DSPP gene in dental pulp cells cultured in a mineralizing medium. The results presented here suggest that DSPP is regulated post-transcriptionally by mir32, mir885-5p and mir586 during odontoblast differentiation.

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Section: Identification Of Mirnas That Regulate Dspp By Qrt-pcrmentioning

confidence: 68%

miRNA expression profiling identifies DSPP regulators in cultured dental pulp cells

Huang

Xu²,

Gao³

et al. 2011

Int J Mol Med

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“…Graphs depict mean and standard deviation from 3 different cell donors; *p < 0.05. (Lindroos et al, 2008) and DSPP expression (Kitagawa et al, 2007;Nomiyama et al, 2007;Pääkkönen et al, 2008). Furthermore, dental pulp cells adjacent to reparative dentin stain more intensely for DSP and DPP than undifferentiated cells within the dental pulp tissue, indicating an up-regulation of these proteins during reparative dentinogenesis .…”

Section: Discussionmentioning

confidence: 85%

TNF-α Promotes an Odontoblastic Phenotype in Dental Pulp Cells

et al. 2009

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“…A detailed characterization of this cell line concerning odontoblast differentiation was thoroughly described previously (Kitagawa et al, 2007). For odontoblast differentiation, HDPCs were cultured in osteogenic medium (OM, 50 µg/mL ascorbic acid and 10 mM β-glycerophosphate, and 0.1 µM dexamethasone) for 14 days as previously described (Wei et al, 2007).…”

Section: Cell Culturementioning

confidence: 99%

NOD2 Mediates Odontoblast Differentiation and RANKL Expression

Lee

Kim

et al. 2014

J Dent Res

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The precise regulation of odontoblast differentiation and osteoclastogenic cytokine expression in human dental pulp cells (HDPCs) is crucial for the pathology of bacteria-related pulpitis. Although the up-regulation of nucleotide-binding oligomerization domain-containing protein 2 (NOD2) has been reported in inflamed human dental pulps, the role of NOD2 in the differentiation of HDPCs remains unclear. Here, we show the involvement of NOD2 in odontoblast differentiation together with osteoclastogenic cytokine expression in HDPCs. Treatment with muramyl dipeptide (MDP), a known NOD2-agonist, significantly inhibited odontoblast differentiation of HDPCs, as revealed by reduced ALP activity, osteoblast/ odontoblast marker expression, and mineralized nodule formation. Importantly, the forced down-regulation of NOD2 by small interfering RNA (siRNA) recovered MDP-down-regulated odontoblast differentiation. MDP-elicited suppression of odontoblast differentiation resulted from the increased expression of MKP-1 protein and the subsequent decline of MAPKs phosphorylation, which is a prerequisite for odontoblast differentiation. Furthermore, we found that MDP treatment elevated the expression of osteoclastogenic cytokines in HDPCs, which was also reversed by NOD2 silencing. Analysis of these data, taken together, suggests that the regulation of NOD2 expression upon MDP challenge might serve as an intrinsic mechanism that underlies the hindered dentin formation and accelerated dentin resorption in bacterial infection-mediated pulpitis.Abbreviations: HDPCs, human dental pulp cells; NOD2, nucleotide-binding oligomerization domain-containing protein 2; MDP, muramyl dipeptide; siRNA, small interfering RNA; MAPKs, mitogen-activated protein kinases; MKP-1, MAPK phosphatase-1; BMMs, bone-marrow-derived macrophages; and CM, conditioned medium.

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Immortalization and characterization of human dental pulp cells with odontoblastic differentiation

Cited by 105 publications

References 21 publications

miRNA expression profiling identifies DSPP regulators in cultured dental pulp cells

miRNA expression profiling identifies DSPP regulators in cultured dental pulp cells

TNF-α Promotes an Odontoblastic Phenotype in Dental Pulp Cells

NOD2 Mediates Odontoblast Differentiation and RANKL Expression

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