Immortalisation of human bone marrow endothelial cells: characterisation of new cell lines

Rood, P. M. L.; Calafat, Jero; Borne, von dem; Gerritsen, Winald R.; Schoot, van der C.E.

doi:10.1046/j.1365-2362.2000.00672.x

Cited by 63 publications

(72 citation statements)

References 28 publications

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“…For proliferation, adhesion, motility, and invasion assays, the insulin concentration in the serum-free culture medium was decreased to 5 mg/l. Human bone marrow endothelial cells (HBMEC-60) were cultured in endothelial cell culture medium in culture flasks coated with fibronectin as previously described (Rood et al 2000).…”

Section: Cell Culturementioning

confidence: 99%

“…Immortalized HBMEC-60 (Rood et al 2000) were cultured on fibronectin-coated 96-well plates until confluent. Then they were starved in serum-free medium for 3 h. In the meantime, prostate tumor cells were labeled with 4 mg/ml calcein AM for 1 h, washed in PBS, resuspended in medium 199 supplemented with 0.1% BSA, and added to the endothelial cells.…”

Section: Epithelial/endothelial Interaction Assaymentioning

confidence: 99%

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Novel mechanism of IGF-binding protein-3 action on prostate cancer cells: inhibition of proliferation, adhesion, and motility

Massoner¹,

Colleselli²,

Matscheski³

et al. 2009

Endocrine-Related Cancer

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IGF-binding protein-3 (IGFBP-3) is a modulator of the IGF-signaling pathway and was described as an anti-cancer agent in prostate cancer. The molecular mechanisms underlying these effects remained, however, largely undefined. We analyzed the influence of recombinant IGFBP-3 on cell proliferation of PC3, Du145, and LNCaP prostate cancer cells. As expected, IGFBP-3 inhibited IGF-stimulated cell proliferation by blocking IGF-mediated proliferation signals, but we observed an IGF-independent inhibitory effect of IGFBP-3 on prostate cancer cell proliferation in long-term cultures. We further investigated the influence of IGFBP-3 on adhesion, motility, and invasion of prostate cancer cells using adhesion assays, live-cell imaging techniques, and matrigel invasion measurements. There was a clear inhibitory effect of IGFBP-3 on tumor cell adhesion to extracellular matrix components in the presence and absence of IGF, whereas cell-cell adhesion was not affected. The same inhibitory effect of IGFBP-3 was determined on cell motility when realtime cell movements were followed. In addition, IGFBP-3 was able to inhibit tumor cell invasion through matrigel. In summary, we show that IGFBP-3 inhibits proliferation, adhesion, migration, and invasion processes of prostate tumor cells. These newly described mechanisms of IGFBP-3 can be of importance for tumor progression and support a role of IGFBP-3 in therapeutic settings.

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Section: Cell Culturementioning

confidence: 99%

Section: Epithelial/endothelial Interaction Assaymentioning

confidence: 99%

Novel mechanism of IGF-binding protein-3 action on prostate cancer cells: inhibition of proliferation, adhesion, and motility

Massoner¹,

Colleselli²,

Matscheski³

et al. 2009

Endocrine-Related Cancer

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show abstract

“…Human BMEC cell lines 4LHBMEC 28 (used in most experiments), TrHBMEC 10 and HBMEC60 30 (kindly provided by Dr CE van der Schoot, CLB, Amsterdam, The Netherlands) were cultured in endothelial cell culture medium consisting of medium 199, 10% new born calf serum, 1% glutamine (Gibco, Grand Island, NY, USA), 10% human pooled serum (CLB, or BioWhittaker, Walkersville, MD, USA), 0.15 mg/ml crude endothelial cell growth factor and 5 IU/ml heparin at 37°C and 5% CO 2 in six-well plates coated with fibronectin (kindly provided by Dr JA van Mourik (CLB)) and passaged twice a week by detachment by trypsin-EDTA (BioWhittaker). HUVEC were isolated as described by Jaffe et al 31 from umbilical cords obtained from the department of Gynecology and Obstetrics (Vrije Universiteit Medical Center, Amsterdam, The Netherlands) after informed consent.…”

Section: Cell Culturesmentioning

confidence: 99%

Proteoglycans on bone marrow endothelial cells bind and present SDF-1 towards hematopoietic progenitor cells

et al. 2003

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“…Human colon carcinoma cell lines HT-29 and Caco-2 were cultured in RPMI 1640 medium and MEM, respectively; both media were supplemented with 10% FCS. Human bone marrow endothelial cells (HBMEC-60; Rood et al, 2000), kindly provided by Dr. C. E. van der Schoot (University of Amsterdam, Amsterdam, the Netherlands), were maintained in endothelial cell culture medium supplemented with 20% FCS, 0.4% endothelial cell growth supplement/heparin, 0.1 ng/ml epidermal growth factor, 1 g/ml hydrocortisone, 1 ng/ml fibroblast factor, 50 ng/ml amphotericin B, and 50 g/ml gentamicin (PromoCell, Heidelberg, Germany).…”

Section: Cell Lines and Cell Culturementioning

confidence: 99%

Differential Expression of β-Galactoside α2,6 Sialyltransferase and Sialoglycans in Normal and Cirrhotic Liver and Hepatocellular Carcinoma

Cao

Merling

Crocker

et al. 2002

Laboratory Investigation

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SUMMARY:Sialyltransferases sialylate plasma glycoproteins in hepatocytes and may (as hepatic key enzymes) constitute markers for liver diseases. We examined expression of the prevalent ␣2,6 sialyltransferase (ST6Gal I) and sialoglycans in normal liver, cirrhotic liver, and hepatocellular carcinoma (HCC) using a new ST6Gal I-specific mAb and recombinant fusion proteins of CD22 and sialoadhesin recognizing ␣2,6-or ␣2,3-sialylated glycans in immunohistology and flow cytometry. In normal and cirrhotic liver, ST6Gal I and sialoglycans were localized in the Golgi region of hepatocytes surrounding the bile canaliculi and along the bile canaliculi, respectively. Sialoglycans were additionally recognized in Kupffer cells, bile ducts, endothelial cells, and oval cells. Well-differentiated and moderately differentiated HCC showed Golgi and diffuse cytoplasmic staining of ST6Gal I and sialoglycans, whereas the cytoplasmic staining for ST6Gal I and sialoglycans was decreased or even absent in poorly differentiated HCC. Detection of sialoglycans by the recombinant fusion proteins in Western blots of cell lysates derived from cell lines revealed two major double bands of sialoglycoproteins at 65 and 120 kDa for hepatocytes, three major bands at 54, 49, and 44 kDa for colonic epithelial cells, and one band at 60 kDa for endothelial cells. Our results describe the expression patterns of ST6Gal I and sialoglycans in various liver tissues and demonstrate an altered expression of these structures between benign and malignant hepatocellular lesions. (Lab Invest 2002, 82:1515-1524.

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Immortalisation of human bone marrow endothelial cells: characterisation of new cell lines

Cited by 63 publications

References 28 publications

Novel mechanism of IGF-binding protein-3 action on prostate cancer cells: inhibition of proliferation, adhesion, and motility

Novel mechanism of IGF-binding protein-3 action on prostate cancer cells: inhibition of proliferation, adhesion, and motility

Proteoglycans on bone marrow endothelial cells bind and present SDF-1 towards hematopoietic progenitor cells

Differential Expression of β-Galactoside α2,6 Sialyltransferase and Sialoglycans in Normal and Cirrhotic Liver and Hepatocellular Carcinoma

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