Immobilizing Alcalase® Enzyme onto Magnetic Nanoparticles

Abstract

In recent years, magnetic nanoparticles (MNPs) have been applied to numerous biological systems. The nanoparticles are particularly useful in separating biological molecules due to its low price, scalable ability and very little interference. Here, MNPs, which can efficiently separate biocatalysts from reaction media by external magnet, was used to immobilize an alkaline protease (Alcalase®). Covalent attachment of the enzyme to MNPs began with the functionalization of the MNPs' surface with amines (APTES). Then, glutaraldehyde was introduced to link the MNP surface amines with enzyme surface amine residues, typically lysine. Successful covalent bonds were checked by FT-IR. Our results showed the attached enzyme did not affect superparamagnetic property of MNPs, therefore the MNPs-attached enzyme was easily recovered after the reaction. The immobilized enzyme maintained its activities after 10 times of recycle uses.

Synthesis of Antioxidant Peptides from Melinjo (Gnetum gnemon) Seed Protein Isolated Using Sol-Gel Immobilized Alcalase

“…This loss of proteolytic activity may be caused due to many reasons such as protein denaturation, and or lost enzyme during washing. Similar results have also been reported by Huu et al [33].…”