Immobilized transferrin Fe3O4@SiO2 nanoparticle with high doxorubicin loading for dual-targeted tumor drug delivery

Ding, Wence; Guo, Lin

doi:10.2147/ijn.s51745

Cited by 21 publications

(10 citation statements)

References 34 publications

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“…Similarly, other magnetic NPs such as alloy, also known as bimetallic NPs of iron-cobalt (Fe-Co), iron-platinum (Fe-Pt) have high magnetic properties, super paramagnetism, high curie temperature [34,35]. The exceedingly reported and mostly scrutinized uses of magnetic and bimetallic NPs are for target specific drug delivery [36,37], in magnetic resonance imaging [38,39] and to treat hyperthermia magnetically [40,41].…”

Section: Colloidal Metal Nanoparticles As Important Nanomaterials Formentioning

confidence: 99%

Optimization of Biogenic Synthesis of Colloidal Metal Nanoparticles

Moholkar¹,

Havaldar²,

Potadar³

et al. 2021

Colloids - Types, Preparation and Applications

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Nanotechnology which deals with the synthesis and characterization of dispersed or solid particles in nano-metric range has emerged out to be a novel approach due to its ample applications in biomedical fields. The advancements in the field of nanotechnology and substantial evidences in biomedical applications have led the researchers to explore safe, ecofriendly, rapid and sustainable approaches for the synthesis of colloidal metal nanoparticles. This chapter illustrates superiority of biogenic route of synthesis of nanoparticles over the different approaches such as chemical and physical methods. In biogenic route, plants and microorganisms like algae, fungi, yeast, actinomycetes etc. act as “bio-factories” which reduce the metal precursors and play a crucial role in the synthesis of nanoparticles with distinct morphologies. Thus, the need of hazardous chemicals is eliminated and a safer and greener approach of nanoparticles synthesis can be adopted. This chapter also outlines the effect of optimization of different parameters mainly pH, temperature, time and concentration of metal ions on the nanoparticle synthesis. It is evident that the optimization of various parameters can yield nanoparticles with desired properties suitable for respective biomedical applications.

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Section: Colloidal Metal Nanoparticles As Important Nanomaterials Formentioning

confidence: 99%

Optimization of Biogenic Synthesis of Colloidal Metal Nanoparticles

Moholkar¹,

Havaldar²,

Potadar³

et al. 2021

Colloids - Types, Preparation and Applications

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“…Nonetheless, the performance of Fth in HCC diagnosis is not very well understood. In addition, TfR is overexpressed within cancer cells compared to normal cells for the purpose of obtaining iron for their rapid division and proliferation 13,14. On this basis, TfR of the transfected hepatoma cell is further upregulated driven by the above endogenous contrast agents.…”

Section: Introductionmentioning

confidence: 99%

<p>MR molecular imaging of HCC employing a regulated ferritin gene carried by a modified polycation vector</p>

Zhang¹,

Lu²,

Xu³

et al. 2019

IJN

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Purpose: Early diagnosis is essential for reducing liver cancer mortality, and molecular diagnosis by magnetic resonance imaging (MRI) is an emerging and promising technology. The chief aim of the present work is to use the ferritin gene, modified by the alpha-fetoprotein (AFP) promoter, carried by a highly safe vector, to produce signal contrast on T2-weighted MR imaging as an endogenous contrast agent, and to provide a highly specific target for subsequent therapy. Methods: Polyethyleneimine-β-cyclodextrin (PEI-β-CD, PC) was synthesized as a novel vector. The optimal nitrogen/phosphorus ratio (N/P) of the PC/plasmid DNA complex was determined by gel retardation, biophysical properties and transmission electron microscopy morphological analysis. The transfection efficiency was observed under a fluorescence microscope and analyzed by flow cytometry. Cellular iron accumulation caused by ferritin overexpression was verified by Prussian blue staining, and the resulting contrast imaging effect was examined by MRI. Results: The modified cationic polymer PC was much safer than high molecular weight PEI, and could condense plasmid DNA at an N/P ratio of 50 with suitable biophysical properties and a high transfection efficiency. Overexpression of ferritin enriched intracellular iron. The short-term iron imbalance initiated by AFP promoter regulation only occurred in hepatoma cells, resulting in signal contrast on MRI. The specific target TfR was also upregulated during this process. Conclusion: These results illustrate that the regulated ferritin gene carried by PC can be used as an endogenous contrast agent for MRI detection of hepatocellular carcinoma (HCC). This molecular imaging technique may promote safer early diagnosis of HCC, and provide a more highly specific target for future chemotherapy drugs.

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“…The researchers, thereby, provided evidence for the advantages of the combination of different materials in a core-shell manner, in this case combinatorial drug delivery [36]. Ding et al used a Fe 3 O 4 core and SiO 2 shell, to which they coupled doxirubicin and immobilized Tf, for targeting purposes [37]. This approach was reported to increase the efficiency of uptake, mediated by Tf, as well as an increased efficacy of the applied drug [37].…”

Section: Introductionmentioning

confidence: 99%

“…Ding et al used a Fe 3 O 4 core and SiO 2 shell, to which they coupled doxirubicin and immobilized Tf, for targeting purposes [37]. This approach was reported to increase the efficiency of uptake, mediated by Tf, as well as an increased efficacy of the applied drug [37]. The synthesis approach for PDA/protein core-shell NPs, published by Bergtold et al, is straightforward, stable, reliable, and inexpensive.…”

Section: Introductionmentioning

confidence: 99%

Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing

et al. 2018

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Polydopamine can form biocompatible particles that convert light into heat. Recently, a protocol has been optimized to synthesize polydopamine/protein hybrid nanoparticles that retain the biological function of proteins, and combine it with the stimuli-induced heat generation of polydopamine. We have utilized this novel system to form polydopamine particles, containing transferrin (PDA/Tf). Mouse melanoma cells, which strongly express the transferrin receptor, were exposed to PDA/Tf nanoparticles (NPs) and, subsequently, were irradiated with a UV laser. The cell death rate was monitored in real-time. When irradiated, the melanoma cells exposed to PDA/Tf NPs underwent apoptosis, faster than the control cells, pointing towards the ability of PDA/Tf to mediate UV-light-induced cell death. The system was also validated in an organotypic, 3D-printed tumor spheroid model, comprising mouse melanoma cells, and the exposure and subsequent irradiation with UV-light, yielded similar results to the 2D cell culture. The process of apoptosis was found to be targeted and mediated by the lysosomal membrane permeabilization. Therefore, the herein presented polydopamine/protein NPs constitute a versatile and stable system for cancer cell-targeting and photothermal apoptosis induction.

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Immobilized transferrin Fe3O4@SiO2 nanoparticle with high doxorubicin loading for dual-targeted tumor drug delivery

Cited by 21 publications

References 34 publications

Optimization of Biogenic Synthesis of Colloidal Metal Nanoparticles

Optimization of Biogenic Synthesis of Colloidal Metal Nanoparticles

<p>MR molecular imaging of HCC employing a regulated ferritin gene carried by a modified polycation vector</p>

Polydopamine/Transferrin Hybrid Nanoparticles for Targeted Cell-Killing

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