Immobilized Stenotrophomonas maltophilia KB2 in Naproxen Degradation

Abstract

Immobilization is a commonly used method in response to the need to increase the resistance of microorganisms to the toxic effects of xenobiotics. In this study, a plant sponge from Luffa cylindrica was used as a carrier for the immobilization of the Stenotrophomonas maltophilia KB2 strain since such a carrier meets the criteria for high-quality carriers, i.e., low price and biodegradability. The optimal immobilization conditions were established as a temperature of 30 °C, pH 7.2, incubation time of 72 h, and an optical density of the culture of 1.4. The strain immobilized in such conditions was used for the biodegradation of naproxen, and an average rate of degradation of 3.8 µg/hour was obtained under cometabolic conditions with glucose. The obtained results indicate that a microbiological preparation based on immobilized cells on a luffa sponge can be used in bioremediation processes where it is necessary to remove the introduced carrier.

Application of Immobilized Biocatalysts in the Biotransformation of Non-Steroidal Anti-Inflammatory Drugs

“…Planococcus sp. S5, Bacillus thuringiensis B1(2015b), Pseudomonas moorei KB4, and Stenotrophomonas maltophilia KB2 were immobilized separately through the adsorption on the surface of the loofah sponge, according to Dzionek et al [20], Dzionek et al [21], Surma et al [22], Wojcieszy ńska et al [23], respectively. The initial optical density of the cultures used during the immobilization of Planococcus sp.…”

“…In previous studies, the authors successfully immobilized strains capable of degrading selected NSAIDs on the plant sponge Luffa cylindrica and characterized their properties [20][21][22][23]. The choice of plant sponge from Loofah cylindrica was dictated by the fact that it is a cheap, biodegradable, and biocompatible material with a hydrophilic surface on which there are many functional groups.…”