Immobilized plant cells

Rosevear, A.; Lambe, C. A.

doi:10.1007/bfb0002535

Cited by 27 publications

(8 citation statements)

References 36 publications

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“…The amount of time required to achieve maximum biomass accumulation by the substratum will decrease with greater initial inoculum densities. The immobilization of cultured plant cells on an inert support has been recognized as a means by which the environment of the cells can be easily manipulated (Brodelius and Mosbach 1982;Rosevear and Lambe 1985). Two general responses have been implicated as resulting from the immobilization process: (1) a slower growth rate of the immobilized ceils relative to suspended cells under otherwise identical conditions, and (2) increased yields of specific secondary metabolites relative to those in suspension-cultured cells.…”

Section: R E S U L T S and Discussionmentioning

confidence: 99%

“…The typically observed increase in secondary product accumulation by immobilized plant cells has been attributed to increased intercellular contact (Brodelius and Mosbach 1982;Rhodes 1985;Rosevear and Lambe 1985). It is suggested that this increased cellular organization promotes biochemical differentiation of the cultured plant cells resulting in reestablishment of the full biochemical complexity of the intact plant as represented by a more complete phytochemical profile .…”

Section: Introductionmentioning

confidence: 99%

“…The benefits of immobilizing cultured plant cells, in terms of the development of a commercialscale bioreactor, can be classified as those resulting from (a) retention of the catalyst within the reactor, (b) high concentration of catalytic activity, (c) microenvironmental control, (d) quantitative and rapid removal of biocatalyst from the product, and (e) uncoupling of growth and production phases. These advantages have been examined in detail in numerous reviews (Brodelius and Mosbach 1982;Rhodes 1985;Rosevear and Lambe 1985).…”

Section: Introductionmentioning

confidence: 99%

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Immobilization of cultured Catharanthus roseus cells using a fibreglass substratum

Facchini

DiCosmo

1990

Appl Microbiol Biotechnol

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Cultured C a t h a r a n t h u s roseus cells were immobilized using geometrically identical needled fibreglass mats prepared with a range of surface coatings. The phenyl (PS), polyglycol (PG), aldehyde (CHO), alkyl (CTMS), and silanol (AW) coatings, along with the untreated glass (HC) surface, produced surfaces with a range of surface tensions. The immobilization efficiency of the substratum, measured as the percentage of cells immobilized, increased with increasing substratum surface tension in the order PS < PG < CHO < CTMS < AW < HC. The dependence of immobilization efficiency on substratum surface tension can be described using a thermodynamic model of adhesion that considers the extent of plant cell adhesion to be a function of the surface tensions of the substratum, the suspending liquid, and the plant cells. In addition, this dependence also demonstrates the fundamental role of adhesion in the immobilization process involving a glass fibre matrix. However, cell entrapment processes are also implicated. The untreated glass fibre substratum (HC), which demonstrated the greatest immobilization efficiency, was used for further characterization of the immobilization strategy. Maximum inoculum biomass was determined to be approximately 1.9 g cells (fresh weight)/g substratum (dry weight) to achieve greater than 90% immobilization efficiency. The growth rate of immobilized cultures was slower than suspension cultures, probably due to mass transfer limitations. Production of the indole alkaloids, tryptamine, catharanthine, and ajmalicine, was also suppressed relative to suspension-cultured cells. These results are considered in relation to other immobilization strategies and their apparent effects on cellular processes.

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Section: R E S U L T S and Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Immobilization of cultured Catharanthus roseus cells using a fibreglass substratum

Facchini

DiCosmo

1990

Appl Microbiol Biotechnol

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“…In this method, plant cells are immobilized in or on a supporting material such agar, agarose, calcium alginate, glass, or polyurethane foam (Lindsey and Yeoman 1985). Plant cell immobilization has a number of other processing advantages, such as re-use of cells (Brodelius et al 1979), cultivation at higher cell densities, and separation of product and cells (Rosevear and Lambe 1983). Immobilization has been shown to improve the production levels of various secondary metabolites (Aoyagi et al 1998;Villegas et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Sukito¹,

Tachibana

2016

Bioresour. Bioprocess.

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Background: Bilobalide and ginkgolides are reported to be present only in Ginkgo biloba. However, only trace amounts of bilobalide and ginkgolides are contained in the ginkgo leaves. Nowadays, there has been considerable interest in plant cell cultures as a potential alternative to traditional agriculture for the industrial production of secondary metabolites. Much effort has been put into the use of in vitro cultures as one attractive biotechnological strategy for producing bilobalide and ginkgolides of commercial interest. The aim of this study was to enhance the production of bilobalide and ginkgolides A, B, and C in cell cultures of G. biloba using immobilized cell cultures and the process of elicitation.Results: Based on the effect of the immobilization components on the free cell suspension cultures, it was considered that the jute fiber acted as an elicitor and forced the cells to release the product into the culture media. The resulting biomass was approximately 1.4 times higher than in the cell suspension cultures, and the production of bilobalide and ginkgolides A, B, and C increased 5.0, 3.3, 6.1, and 4.1 times, respectively. Eliciting with methyl jasmonate (MJ) and salicylic acid (SA) in the immobilized cells enhanced bilobalide and ginkgolides A, B, and C, compared with the unelicited controls. The highest accumulation was observed using a combination of 0.1 mM MJ + 0.1 mM SA in the immobilized cells, which produced 1.78, 1.95, 2.05, and 2.95 times more bilobalide and ginkgolides A, B, and C, respectively, than the controls. Conclusions:The positive effects of immobilized cell cultures using jute fiber and the synergism effect of SA and MJ on immobilized cells of G. biloba appear to be the optimal conditions for continuous in vitro production for commercial purposes. This is the first report on analyzing the effects of jute fiber as immobilized cell material on G. biloba cell cultures and the synergism of MJ and SA on bilobalide and ginkgolide production.

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“…Upon immobilization, an enzyme may have different catalytic activity caused by a conformational change during immobilization, the change in microenvironment of the matrix compared to the bulk phase, interaction between the matrix and enzyme, and diffusion limitation imposed by the matrix (Rosevear et al, 1987). It is important to know how immobilization affects enzyme behavior, in order to establish appropriate reaction conditions.…”

Section: Introductionmentioning

confidence: 99%

Characterization of sol‐gel entrapped chlorophyllase

Kermasha

Neufeld

2006

Biotech & Bioengineering

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Immobilization of membrane proteins remains a challenge compared to soluble proteins. The membrane protein-chlorophyllase was successful entrapped in tetramethoxysilane (TMOS)-based sol-gel in the presence of lipid. Activity was examined against mixing rate, incubation temperature, time, substrate, acetone, and canola oil concentration. The external mass transfer of chlorophyll is not the rate-limiting step at higher mixing rates. Stability against temperature and acetone as denaturant was enhanced. In spite of the fact that an initial reaction lag phase was observed, 20% more chlorophyll was hydrolyzed, compared to reaction with free enzyme by the end of a 12 h assay. The initial lower activity demonstrated by entrapped chlorophyllase is likely due to the diffusion resistance of chlorophyll into and within the entrapment matrix. This hypothesis was substantiated by a low diffusion coefficient on the order of 10(-14) m(2)/s obtained for chlorophyll in nanoporous sol-gel particles. Pore size distribution of nanoporous wet TMOS-based sol-gel with or without protein was determined by thermoporometry. The change in pore morphology upon doping with chlorophyllase suggests that protein acts as a template during the sol-gel process.

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Immobilized plant cells

Cited by 27 publications

References 36 publications

Immobilization of cultured Catharanthus roseus cells using a fibreglass substratum

Immobilization of cultured Catharanthus roseus cells using a fibreglass substratum

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Characterization of sol‐gel entrapped chlorophyllase

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