Immobilized phospholipid capillary electrophoresis for study of drug–membrane interactions and prediction of drug activity

Mei, Jie; Xu, Jie; Xiao, Yuxiu; Zhang, Qianrui; Feng, Yu‐Qi

doi:10.1016/j.talanta.2007.10.037

Cited by 23 publications

(16 citation statements)

References 26 publications

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“…[20,21]. Chloroform solution of 60 mg soybean phosphatidylcholine and 20 mg cholesterol was dried in a round-bottomed flask by vacuum evaporation at 50°C to yield a thin lipid film on the inner surface of the flask.…”

Section: Liposome Preparationmentioning

confidence: 99%

Monoamine Oxidase Inhibitor Screening by Proteoliposome Capillary Electrophoresis

Qing

et al. 2011

Chromatographia

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In this study, a new strategy for screening of enzyme inhibitors based on the interaction between enzyme and substrate was established. The bioactive proteoliposome reconstituted by monoamine oxidase (MAO) and liposome was added in the running buffers of capillary electrophoresis (CE) as pseudostationary phase to simulate the interaction between MAO and its substrate kynuramine (Kyn). The results showed that the relative migration time ratio (RMTR) of Kyn decreased obviously with increase of the proteoliposome, indicating strong interaction between proteoliposome and Kyn. After adding MAO inhibitors into the running buffers containing proteoliposome, the decline of the RMTR value of Kyn became slower. This occurred because MAO was inhibited to some degree by the inhibitors, weakening the interaction between MAO and Kyn. However, adding compounds which do not inhibit MAO activity into the running buffers did not affect the RMTR of Kyn obviously. The results obtained demonstrate that the method developed could characterize the inhibition of MAO inhibitors. Compared with traditional inhibitor screening methods, it does not need incubation outside the column to complete the enzyme catalytic reaction, so analysis is faster and less sample is consumed. With further development, the method might be chosen as a useful tool for screening of membrane protein inhibitors and identification of proteins associated with various diseases.

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Section: Liposome Preparationmentioning

confidence: 99%

Monoamine Oxidase Inhibitor Screening by Proteoliposome Capillary Electrophoresis

Qing

et al. 2011

Chromatographia

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“…Analytes were detected at 210 nm. It was assumed that the data obtained by IPCA can be used for the study of the drug-membrane interactions and for the prediction of the biological activity of the drugs under investigation (Mei et al, 2008a;Mei et al, 2008b).…”

Section: Immobilized Phospholipid Capillary Electrophoresismentioning

confidence: 99%

“…Measurements indicated that the intra-and inter-capillary variations in electro-osmotic flow were 4.02% RSD (n = 30) and 6.72% RSD (n = 4), respectively. It was established that the migration time determined in this CE system can be applied for the in vitro prediction of the fraction of dose of drugs (Mei et al, 2008a;Mei et al, 2008b).…”

Section: Capillary Electrokinetic Chromatographymentioning

confidence: 99%

Liposomes in chromatography

Cserháti

Szőgyi

2010

Biomedical Chromatography

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The newest achievements in the application of liposomes in different chromatographic technologies such as high-performance liquid chromatography and electrically driven separation methods was compiled and critically evaluated. The employment of chromatographic methodologies for the determination of molecular parameters of biological importance is also discussed in detail. The future trends of the use of liposomes is also shortly discussed.

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“…Many further studies have proven the usefulness of IAMs to predict octanol/water partition coefficients, and drug permeability across biological membranes [9][10][11][12][13][14][15]. So far, phospholipidcoated columns have been applied to study of "in vitro" behavior of (−)-epicatechin conjugates [16], various model drugs [17,18], flavonoids [19], statins [20], NSAIDs [21].…”

Section: Introductionmentioning

confidence: 99%

Thermodynamic study of new antiepileptic compounds by combining chromatography on the phosphatidylcholine biomimetic stationary phase and differential scanning calorimetry

Flieger

Trębacz

Pizoń

et al. 2019

J of Separation Science

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Liquid chromatography coupled to spectrophotometric detection of new antiepileptic compounds, 1,2,4‐triazole‐3‐thione derivatives, on immobilized artificial membrane phosphatidylcholine is reported. The curves representing the relationship between ln k versus 1/T generated under isocratic conditions by the use of methanol and acetonitrile‐containing eluent systems have been constructed in order to determine the thermodynamic parameters: the enthalpies, entropies and the relative free energies. The hydrocarbon chains of analytes significantly influenced the membrane behavior of the whole molecules. Excellent correlations of the theoretical lipophilicity with the experimental thermodynamic descriptors, have confirmed contribution of the hydrophobic interactions in the retention process. However, presence of sulfur or oxygen as heteroatoms at R1 substituents in the 1,2,4‐triazole ring appears to be responsible for more pronounced selectivity of these compounds on the phosphatidylcholine stationary phase. Molecular dynamics simulations revealed the selective preferences of the phosphatidylcholine with respect to the compounds with either ether of sulfide moieties. Experimental and theoretical set‐ups resulted in corresponding outcomes.

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Immobilized phospholipid capillary electrophoresis for study of drug–membrane interactions and prediction of drug activity

Cited by 23 publications

References 26 publications

Monoamine Oxidase Inhibitor Screening by Proteoliposome Capillary Electrophoresis

Monoamine Oxidase Inhibitor Screening by Proteoliposome Capillary Electrophoresis

Liposomes in chromatography

Thermodynamic study of new antiepileptic compounds by combining chromatography on the phosphatidylcholine biomimetic stationary phase and differential scanning calorimetry

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