Immobilized magnetic beads based multi-target affinity selection coupled with high performance liquid chromatography–mass spectrometry for screening anti-diabetic compounds from a Chinese medicine “Tang-Zhi-Qing”

Tao, Yi; Chen, Zhui; Zhang, Yufeng; Wang, Yi; Cheng, Yiyu

doi:10.1016/j.jpba.2013.02.024

Cited by 48 publications

(21 citation statements)

References 37 publications

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“…The precision of the established approach at each QC concentration was determined as the relative standard deviation (RSD). The suitability of the precision and accuracy was assessed by the following criteria: the RSD should not exceed 15 % and the accuracy should be within 15 % of the actual values for the QC sample [17].…”

Section: Accuracy Precision and Matrix Effectmentioning

confidence: 99%

“…The matrix effect was evaluated by comparing the peak area of buffer solution spiked with standard solution of rutin to that of different organ extracts (stem, leaf, and flower) of D. officinale spiked with the standard solutions of rutin at three QC levels [17].…”

Section: Accuracy Precision and Matrix Effectmentioning

confidence: 99%

“…The enzyme-binding capacity is not equivalent to inhibitory effects due to the fact that the enzymatic binding sites are not necessary for substrate transformation [17]. Docking studies were performed to investigate the interaction between the active constituents and the active site of the lipase.…”

Section: Docking Studiesmentioning

confidence: 99%

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Ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry for screening lipase binders from different extracts of Dendrobium officinale

Tao

Cai

et al. 2015

Anal Bioanal Chem

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Pancreatic lipase plays essential roles in the digestion, transport, and processing of dietary lipids in humans. Inhibition of pancreatic lipase leading to the decrease of lipid absorption may be used for treating obesity. In the present study, a new approach of ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry was established for rapidly detecting lipase binders from different extracts of medicinal plants. Rutin, a model inhibitor of lipase, was selected to optimize the screening conditions, including ion strength, temperature, pH, and incubation time. Meanwhile, the specificity of the approach was investigated by using denatured lipase and inactive compound emodin. The optimal screening conditions were as follows: ion strength 75 mM, temperature 37 °C, pH 7.4, and incubation time 10 min. Furthermore, linearity, accuracy, precision, and matrix effect of the approach were well validated. Finally, lipase binders were screened from different extracts of Dendrobium officinale by applying the established approach and were subsequently subjected to traditional lipase inhibitory assay. Eleven lipase inhibitors were identified, eight of which, namely naringenine, vicenin II, schaftoside, isoschaftoside, isoquercetrin, kaempferol 3-O-β-D-glucopyranoside, vitexin 2″-O-glucoside, and vitexin 2″-O-rhamnoside, were reported for the first time. In addition, docking experiments were performed to determine the preferred binding sites of these new lipase inhibitors.

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Section: Accuracy Precision and Matrix Effectmentioning

confidence: 99%

Section: Accuracy Precision and Matrix Effectmentioning

confidence: 99%

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Ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry for screening lipase binders from different extracts of Dendrobium officinale

Tao

Cai

et al. 2015

Anal Bioanal Chem

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“…Several types have been described in the literature; some with porous structure to specifically retain target analytes, for example restricted access materials (RAM) or molecular imprinted polymers (MIP); others with very specific functional groups (e.g. immunosorbents), or unspecific materials such as non‐polar coatings (C8, C18,…), ion exchange groups, or moieties that provide aromatic interactions. The extraction procedure is similar to solid‐phase extraction (SPE); i.e., loading, washing, and elution, with the added advantage that the suspended magnetic beads can be handled as a liquid.…”

Section: Introductionmentioning

confidence: 99%

“…Because of the organic solvent in the resulting extract, samples usually have to be further diluted prior to LC‐MS analysis. Strong hydrophilic compounds can also be captured by implementing extraction materials of high specificity; for example MIP or immunospecific sorbents . While these materials give assays with high sensitivity, the materials are difficult to synthesize and can give template bleeding (MIP).…”

Section: Introductionmentioning

confidence: 99%

Magnetic beads as an extraction medium for simultaneous quantification of acetaminophen and structurally related compounds in human serum

Bylda

Velichkova

Bolle

et al. 2014

Drug Testing and Analysis

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This paper describes a sample preparation method that complements a previously published liquid chromatography-tandem mass spectrometry (LC-MS/MS) assay for acetaminophen and eight structurally-related compounds in human serum (C. Bylda, R. Thiele, U. Kobold, D.A. Volmer. Drug Test. Anal. 2014, 6, 451). The analytes (acetaminophen [APAP] + metabolites acetaminophen-glucuronide [APG], -cysteine [APC], -mercapturate [APM] and -cysteine [APC], structurally similar analogues phenacetin and p-phenetidine, as well as tricyclic antidepressants imipramine and amitryptiline) were extracted from serum using magnetized hyper-crosslinked polystyrene particles. The sample preparation protocol was developed by means of a design of experiments (DoE) statistical approach. Using three representative compounds from the analyte panel with different polarities (high, medium, and low), two screening designs were used to identify factors that exhibited significant impact on recovery of the analytes. These parameters were then optimized to permit extraction of the complete target panel exhibiting a broad range of chemical polarities. Liquid chromatographic separations were achieved by gradient elution using a pentafluorphenyl column with subsequent detection by electrospray ionization-triple quadrupole mass spectrometry in multiple reaction monitoring (MRM) mode. The method was linear over the range 0.1-100 µg/mL for APAP, APG, p-phenetidine and phenacetin, 0.03-50 µg/mL for APS, and 0.01-10 µg/mL for APM, APC, imipramine and amitriptyline, with R(2) > 0.99. The assay exhibited good precision with CVs ranging from 2 to 9% for all analytes; the accuracy was assessed by comparing two LC-MS/MS methods using a set of 68 patient samples.

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A rapid and sensitive method based on magnetic beads for the detection of hepatitis B virus surface antigen in human serum

et al. 2013

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Current clinically assays, such as enzyme-linked immunosorbent assay and chemiluminescence immunoassay, for hepatitis B surface antigen (HBsAg) are inferior in terms of either sensitivity and accuracy or rapid and high-throughput analysis. A novel assay based on magnetic beads and time-resolved fluoroimmunoassay was developed for the quantitative determination of HBsAg in human serum. HBsAg was captured using two types of anti-HBsAg monoclonal antibodies (B028, S015) immobilized on to magnetic beads and detected using europium-labeled anti-HBsAg polyclonal detection antibody. Finally, the assay yielded a high sensitivity (0.02 IU/mL) and a wide dynamic range (0.02-700 IU/mL) for HBsAg when performed under optimal conditions. Satisfactory accuracy, recovery and specificity were also demonstrated. The intra- and interassay coefficients of variation were 4.7-8.7% and 3.8-7.5%, respectively. The performance of this assay was further assessed against a well-established commercial chemiluminescence immunoassay kit with 399 clinical serum samples. It was revealed that the test results for the two methods were in good correlation (Y = 1.182X - 0.017, R = 0.989). In the current study, we demonstrated that this novel time-resolved fluoroimmunoassay could be used: as a highly sensitive, automated and high-throughput immunoassay for the diagnosis of acute or chronic hepatitis B virus infection; for the screening of blood or organ donors; and for the surveillance of persons at risk of acquiring or transmitting hepatitis B virus.

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Immobilized magnetic beads based multi-target affinity selection coupled with high performance liquid chromatography–mass spectrometry for screening anti-diabetic compounds from a Chinese medicine “Tang-Zhi-Qing”

Cited by 48 publications

References 37 publications

Ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry for screening lipase binders from different extracts of Dendrobium officinale

Ultrafiltration coupled with high-performance liquid chromatography and quadrupole-time-of-flight mass spectrometry for screening lipase binders from different extracts of Dendrobium officinale

Magnetic beads as an extraction medium for simultaneous quantification of acetaminophen and structurally related compounds in human serum

A rapid and sensitive method based on magnetic beads for the detection of hepatitis B virus surface antigen in human serum

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